(E)4-[4-N,N-dimethylaminophenyl]but-3-en-2-one (DMAP) treatment inhibits the radiation-induced micronucleus formation in bone marrow of BALB/c mice

Jagetia, Ganesh Chandra; Jacob, Preenu Sunil; Rao, M. N. A.

doi:10.1016/0027-5107(94)90168-6

Cited by 12 publications

(9 citation statements)

References 38 publications

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“…Also, b-carotene prevents X-ray-induced MN induction in HPBLs [Umegaki et al, 1994], and flavonoids of Ocimum sanctum, like orientin and vicenin, protect against radiation-induced MN formation in human lymphocytes [Vrinda and Uma Devi, 2001]. Similarly, (E)-4-[4-N,N-dimethylaminophenyl]but-3-en-2-one (DMAP), a synthetic compound, abana, and naringin reduce radiation-induced MN formation in mouse bone marrow [Jagetia et al, 1994;Jagetia and Aruna, 1997;Jagetia and Reddy, 2002]. MGN did not have any cytotoxic effects on HPBLs as the PI remained unaltered after MGN treatment in our study.…”

Section: Discussionmentioning

confidence: 96%

Effect of mangiferin on radiation-induced micronucleus formation in cultured human peripheral blood lymphocytes

Jagetia

Venkatesha

2005

Environ. Mol. Mutagen.

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Irradiation causes a variety of lesions in important biomolecules of the cell through generation of free radicals leading to genomic instability. DNA strand breaks, acentric fragments, or defective kinetochores are manifested as micronuclei after the first cell division. Chemicals that can trap free radicals may reduce the deleterious effects of ionizing radiation. Mangiferin (MGN), a glucosylxanthone derived from Mangifera indica (mango), was investigated for its ability to reduce the frequency of radiation-induced micronucleated binucleate cells (MNBNCs) in cultured human peripheral blood lymphocytes (HPBLs). HPBL cultures were pretreated with 0, 5, 10, 20, 50, and 100 microg/ml of MGN for 30 min before exposure to 3 Gy of (60)Co gamma-radiation. The maximum decline in radiation-induced micronuclei was observed at a concentration of 50 microg/ml MGN; thereafter, a nonsignificant elevation in MNBNC frequency was observed at 100 microg/ml MGN. Since the lowest MNBNC frequency was observed for 50 microg/ml MGN, dose-response studies were undertaken using this concentration. Irradiation of HPBLs with 0, 1, 2, 3, or 4 Gy of gamma-radiation caused a dose-dependent elevation in the MNBNC frequency, while treatment of HPBLs with 50 microg/ml MGN 30 min before radiation resulted in significant declines in these frequencies. MGN alone did not alter the proliferation index. Irradiation caused a dose-dependent decline in the proliferation index, while treatment of HPBLs with 50 micro/ml MGN significantly elevated the proliferation index in irradiated cells. MGN treatment reduced hydrogen peroxide-induced lipid peroxidation in HPBLs in a concentration-dependent fashion. In cell-free studies, MGN inhibited the induction of (.)OH (hydroxyl), O(2) (.-) (superoxide), DPPH (1,1-diphenyl-2-picrylhydrazyl), and ABTS(.+) (2,2-azino-bis-3-ethyl benzothiazoline-6-sulphonic acid) radicals in a dose-dependent manner. The results of this study indicate that MGN possesses radioprotective properties by suppressing the effects of free radicals.

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Section: Discussionmentioning

confidence: 96%

Effect of mangiferin on radiation-induced micronucleus formation in cultured human peripheral blood lymphocytes

Jagetia

Venkatesha

2005

Environ. Mol. Mutagen.

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“…16.6 ± 5.0* Significantly different compared to corresponding control by Student's t-test, *P < 0.05, **P < 0.01, ***P < 0.001. E(4-[4 N,N-Dimethylaminophenyl]but-3en-one inhibits the radiation-induced micronucleus formation in the bone marrow of mice [27]. Singh et al [28] observed a radioprotective effect of garlic extract on the frequency of gamma-radiation-induced micronuclei.…”

Section: Discussionmentioning

confidence: 97%

Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

Dobrzyńska¹,

Gajewski²

2000

Teratog. Carcinog. Mutagen.

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“…An identical effect has been observed earlier with Agele marmelos extract and mangiferin in HPBLs. 5,43 Likewise, a reduction in polychromatic erythorcytes bearing 2 or more MN has been observed in mice bone marrow treated with (E)4-(4-N, N-dimethyl aminophenyl) but-3-en-2-one, 44 naringin, a citrus bioflavonoid, 47 the extracts of abana, 45 , S. cumini, 24 and A. marmelos, 5 and also mangiferin. 43 The whole berry extract of Hippophae rhamnoides, labeled RH-3, has been found to render antioxidant-mediated radioprotection, by preventing cellular and mitochondrial free radical generation.…”

Section: Discussionmentioning

confidence: 99%

“…A similar effect has been observed earlier. 5,[44][45][46][47] However, a linear quadratic response has also been reported. 23,[48][49][50] Irradiation not only increased the frequency of BNC with 1 MN but also with 2 or multiple MN, indicating that induction of complex multiple sites of DNA damage and production of these lesions was radiation dose dependent.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Radiation-Induced DNA Damage by Jamun, Syzygium cumini, in the Cultured Splenocytes of Mice Exposed to Different Doses of γ-Radiation

2011

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The radioprotective property of 50 mg/kg body weight jamun (Syzygium cumini) extract was studied in the cultured splenocytes of mice exposed to 0, 0.5, 1, 2, 3, or 4 Gy of γ-radiation. The spleens of irradiated mice were removed aseptically and the splenocytes were extracted from the individual spleens and cultured. The micronuclei were prepared 72 hours after irradiation in binucleate splenocytes by blocking cytokinesis with cytochalasin-B. Irradiation of mice resulted in a dose-dependent elevation in the micronucleated splenocytes. The exposure of mice not only elevated splenocytes bearing one micronucleus but also cells bearing 2 and multiple (>2) micronuclei indicating induction of complex DNA damage after irradiation. Oral treatment of mice with 50 mg/kg body weight of jamun leaf extract protected against the radiation-induced micronuclei formation. Jamun extract also protected against the formation of 2 and multiple micronuclei indicating repair or inhibition of complex DNA damage. The assessment of lipid peroxidation in mice brain homogenate has indicated a concentration dependent inhibition of lipid peroxidation by jamun extract. Studies in a cell free system revealed that jamun extract inhibited the formation of OH, O 2 −, DPPH, and ABTS + free radicals in a concentration dependent manner. Our study demonstrates that jamun extract protected mice against the radiation-induced DNA damage and inhibition of radiation-induced free radical formation may be one of the mechanisms of radioprotection.

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(E)4-[4-N,N-dimethylaminophenyl]but-3-en-2-one (DMAP) treatment inhibits the radiation-induced micronucleus formation in bone marrow of BALB/c mice

Cited by 12 publications

References 38 publications

Effect of mangiferin on radiation-induced micronucleus formation in cultured human peripheral blood lymphocytes

Effect of mangiferin on radiation-induced micronucleus formation in cultured human peripheral blood lymphocytes

Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

Inhibition of Radiation-Induced DNA Damage by Jamun, Syzygium cumini, in the Cultured Splenocytes of Mice Exposed to Different Doses of γ-Radiation

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