Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

Dobrzyńska, Małgorzata M.; Gajewski, Antoni

doi:10.1002/(sici)1520-6866(2000)20:3<133::aid-tcm4>3.0.co;2-b

Cited by 16 publications

(5 citation statements)

References 42 publications

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“…4), both Wt and Tg-RGN animals showed a higher incidence of abnormal sperm after radiotherapy, which is in agreement with previous reports describing the effects of X-rays on spermatogenesis [49][50][51].…”

Section: Accepted Manuscriptsupporting

confidence: 91%

The protective effect of regucalcin against radiation-induced damage in testicular cells

et al. 2016

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Section: Accepted Manuscriptsupporting

confidence: 91%

The protective effect of regucalcin against radiation-induced damage in testicular cells

et al. 2016

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“…An understanding of the short-term, as well as the long-term, effects is essential if the causes of infertility and testicular atrophy are to be revealed cytogenetically. Many investigations evaluated effects of different chemicals using shortterm exposure (14,(53)(54)(55)(56). This was why, in the present study, 24 and 48 h applications were chosen for the short-term assay.…”

Section: Discussionmentioning

confidence: 99%

Abnormal sperm morphology in mouse germ cells after short-term exposures to acetamiprid, propineb, and their mixture

Rasgele

2014

Archives of Industrial Hygiene and Toxicology

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Pesticides are one of the most potent environmental contaminants, which accumulate in biotic and abiotic components of ecosystems. Acetamiprid (Acm), a neonicotinoid insecticide, and Propineb (Pro), a dithiocarbamate fungicide, are widely used to control sucking insects and fungal infections on crops, respectively. The present study was undertaken to investigate the genotoxic effects of these compounds, individually and in mixtures, in mouse germ cells by using the sperm morphology assay. Mice were injected intraperitoneally with 0.625, 1.25, and 2.50 µg mL -1 of Acm, 12.5, 25, and 50 µg mL -1 of Pro, and their mixture at the same concentrations over 24 and 48 h. Acm did not significantly increase the percentage of abnormal sperm at any concentration. The frequency of abnormal sperm significantly increased after 24 and 48 h of exposure to 50 µg mL -1 of Pro. The mixtures of 2.50 µg mL -1 of Acm and 50 µg mL -1 of Pro induced sperm abnormalities antagonistically both after 24 and 48 h of exposure. Results suggest that Acm was non-genotoxic for mouse germ cells, while Pro may have been a germ cell mutagen due to the observed increase in the frequency of sperm abnormalities. However, to gain better insight into the mutagenicity and DNA damaging potential of both of these pesticides, further studies at molecular level should be done.

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“…(e) In rats, acrylamide in drinking-water-induced neurotoxicity (<0.5 mg/ kg/day) and reproductive toxicity (2.0 mg/kg/day) were affected by different doses of acrylamide, suggesting that neurotoxicity is the cause or a major contributor to developmental toxicity (10,11). (f ) Other investigators confirmed adverse effects of acrylamide on spermatogenesis (12)(13)(14). (g) NAC and GSH protected against acrylamide-induced morphological transformations of Syrian hamster embryo cells (15,16).…”

Section: Introductionmentioning

confidence: 90%

l-Cysteine, N-Acetyl-l-cysteine, and Glutathione Protect Xenopus laevis Embryos against Acrylamide-Induced Malformations and Mortality in the Frog Embryo Teratogenesis Assay

Rayburn

Friedman

2010

J. Agric. Food Chem.

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Dietary acrylamide is largely derived from heat-induced reactions between the amino group of the free amino acid asparagine and carbonyl groups of glucose and fructose during heat processing (baking, frying) of plant-derived foods such as potato fries and cereals. After consumption, acrylamide is absorbed into the circulation and is then distributed to various organs, where it can react with DNA, neurons, hemoglobin, and essential enzymes. In the present study, we explored the potential of L-cysteine (CySH), N-acetyl-L-cysteine (NAC), reduced glutathione (GSH), and the amino acid glycine (Gly) to protect frog embryos against acrylamide-induced developmental toxicity in the frog embryo teratogenesis assay - Xenopus (FETAX). To test the antiteratogenic potential, based on concentration-response study ranging from 0.07 to 4.22 mM acrylamide in FETAX solution (pH 8.1), we selected concentrations of acrylamide that induced 100% malformations and mortality. At the end of 96 h, we counted survivors and malformed embryos and measured embryo length. The data show that CySH, NAC, and GSH protected the embryos against acrylamide induced malformations and mortality to different degrees. CySH and GSH protected the embryos against both malformations and mortality, whereas NAC protected only against mortality. Gly had no protective effect. Possible mechanisms of the protective effects and the dietary significance of the results of this and related studies for food safety and human health are discussed.

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Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

Cited by 16 publications

References 42 publications

The protective effect of regucalcin against radiation-induced damage in testicular cells

The protective effect of regucalcin against radiation-induced damage in testicular cells

Abnormal sperm morphology in mouse germ cells after short-term exposures to acetamiprid, propineb, and their mixture

l-Cysteine, N-Acetyl-l-cysteine, and Glutathione Protect Xenopus laevis Embryos against Acrylamide-Induced Malformations and Mortality in the Frog Embryo Teratogenesis Assay

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