Dysregulated miR-671-5p / CDR1-AS / CDR1 / VSNL1 axis is involved in glioblastoma multiforme

Abstract

MiR-671-5p is encoded by a gene localized at 7q36.1, a region amplified in human glioblastoma multiforme (GBM), the most malignant brain cancer. To investigate whether expression of miR-671-5p were altered in GBM, we analyzed biopsies from a cohort of forty-five GBM patients and from five GBM cell lines. Our data show significant overexpression of miR-671-5p in both biopsies and cell lines. By exploiting specific miRNA mimics and inhibitors, we demonstrated that miR-671-5p overexpression significantly increases migration and to a less extent proliferation rates of GBM cells. Through a combined in silico and in vitro approach, we identified CDR1-AS, CDR1, VSNL1 as downstream miR-671-5p targets in GBM. Expression of these genes significantly decreased both in GBM biopsies and cell lines and negatively correlated with that of miR-671-5p. Based on our data, we propose that the axis miR-671-5p / CDR1-AS / CDR1 / VSNL1 is functionally altered in GBM cells and is involved in the modification of their biopathological profile.

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

“…MiR-671-5p has been found to be a tumour suppressor in several types of cancers [16,17]. Downregulation of miR-671-5p expression has been found in breast cancer tissues compared with their adjacent tissues [18].…”

Circular RNA hsa_circ_0008344 regulates glioblastoma cell proliferation, migration, invasion, and apoptosis

“…Current studies showed another circRNA hsa_circ_0046701 significantly overexpressed in glioma and functioning as a sponge for miR‐142‐3p to promote carcinogenesis . Interestingly, circRNAs were also been found to be targeted by microRNAs that CDR1‐AS, a miR‐7 sponge, could be degraded by miR‐671‐5p in glioma . Moreover, endogenous circRNAs could encode functional proteins, which was confirmed in glioblastoma that circ‐FBXW7 encoded a novel 21‐kDa protein FBXW7‐185aa and inhibited tumor cell proliferation and cell cycle acceleration .…”

RETRACTED ARTICLE: Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p

“…Another study indicated that circ_0046701 remarkably overexpressed in GMB and exerted as a sponge of miR-142-3p to develop tumorgenesis [11]. Furthermore, circRNA could also be targeted by miRNA, and miR-7 sponge CDR1-AS can be deteriorated by miR-671-5p in GMB [12]. Interestingly, several studies illustrated the function of circPVT1 in many cancers.…”