Circular <scp>RNA</scp> hsa_circ_0008344 regulates glioblastoma cell proliferation, migration, invasion, and apoptosis

Zhou, Jianyin; Wang, Hongxiang; Chu, Junsheng; Huang, Qilin; Li, Guangxu; Yan, Yongmin; Xu, Tao; Chen, Juxiang; Wang, Yuhai

doi:10.1002/jcla.22454

Cited by 28 publications

(25 citation statements)

References 30 publications

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“…CircRNAs are synthesized by backsplicing of downstream donor site with the upstream acceptor site using the canonical spliceosomal signals and machinery [11, 12]. Recently ever growing reports on circRNAs reveal their pivotal roles in fundamental biological pathways by their multiple functional aspects, such as microRNAs (miRNAs) sponges [10, 13–17], cap-independent translation [18–21], modulation of cellular proliferation [19, 22–24], scaffolding the protein activity [12, 25, 26], and competition with linear mRNAs [19].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification

Guria

Kumar

Srikakulam

et al. 2019

BioMed Research International

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Circular RNAs (circRNAs) are newly discovered incipient non-coding RNAs with potential roles in disease progression in living organisms. Significant reports, since their inception, highlight the abundance and putative functional roles of circRNAs in every organism checked for, like O. sativa, Arabidopsis, human, and mouse. CircRNA expression is generally less than their linear mRNA counterparts which fairly explains the competitive edge of canonical splicing over non-canonical splicing. However, existing methods may not be sensitive enough for the discovery of low-level expressed circRNAs. By combining template-dependent multiple displacement amplification (tdMDA), Illumina sequencing, and bioinformatics tools, we have developed an experimental protocol that is able to detect 1,875 novel and known circRNAs from O. sativa. The same method also revealed 9,242 putative circRNAs in less than 40 million reads for the first time from the Nicotiana benthamiana whose genome has not been fully annotated. Supported by the PCR-based validation and Sanger sequencing of selective circRNAs, our method represents a valuable tool in profiling circRNAs from the organisms with or without genome annotation.

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Section: Introductionmentioning

confidence: 99%

Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification

Guria

Kumar

Srikakulam

et al. 2019

BioMed Research International

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“…Zhou JX et al concluded that hsa_circ_0008344(circ-UBAP2) is upregulated in glioblastoma and may contribute to the progression of this malignancy;69 Zhang H et al suggested that circ-UBAP2 sponging miR-143 to promote osteosarcoma progression and implicate its potential in prognosis prediction and cancer therapy;70 Wang ST et al revealed that circ-UBAP2 plays a vital regulatory role in TNBC via the miR-661/MTA1 axis and may serve as a promising therapeutic target for TNBC patients;71 Yin YJ et al concluded that circ-UBAP2 exerts an important role in the proliferation and invasion of human lung cancer. Silencing of circUBAP2 might be a novel target for molecular targeted therapy of patients with lung cancer 72…”

Section: The Associations Between Star Circrnas and A Wide Range Of Dmentioning

confidence: 99%

<p>Star Circular RNAs In Human Cancer: Progress And Perspectives</p>

Cheng

Sun

Wang

et al. 2019

OTT

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Circular RNAs (circRNAs) are a recently discovered subclass of non-coding RNAs (ncRNAs) characterized by a covalently closed loop structure created by reverse splicing. Because they do not have a 5ʹ cap structure and a 3ʹ poly A tail, circRNAs have higher stability, abundance and evolutionary conservation than linear RNA between species. These features produce various potential biological functions of circRNAs, such as miRNA sponges, RNA-binding proteins that form RNA protein complexes. In recent years, more and more studies have shown that circRNAs play a vital role in the occurrence and development of human diseases. At the same time, their enormous potential as a biomarker and therapeutic target is also evolving. The purpose of this review is to summarize existing cancer-associated circRNAs and to try to find circRNAs that are abnormally expressed in many cancers. Therefore, we reviewed previous circRNAs studies related to cancer and selected them by statistics. The eight circRNAs that have the highest frequency in different cancers or involve key pathways are called star circRNAs. Here, we review the classification, features, and functions of emerging star circRNAs, with particular attention to the role of circRNAs in various cancers.

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“…While originally believed to be erroneously generated splicing variants, recent high-throughput sequencing studies have found many circRNAs to be highly expressed in mammalian cells, wherein they are stable and seem to have cell type-specific expression patterns [1,3]. Indeed, many studies have found evidence that these circRNAs in fact play essential regulatory roles in both disease-associated and normal physiological processes [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10

Yang

et al. 2020

BMC Cancer

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Background: Circular RNAs (CircRNAs) are biologically active RNAs. CDR1as is one such circRNA previously reported to be a microRNA-7 (miR-7) sponge, thereby regulating associated gene expression. The specific underlying molecular mechanisms of CDR1as biology, however, remain largely unknown. Methods: We performed CDR1as knockdown in order to explore its function in cell proliferation, migration, the cell cycle, and tumorigenesis. We further employed quantitative proteomic analyses and associated bioinformatics strategies to globally assess CDR1as-regulated proteins (CRPs). Western blotting and immunofluorescence staining were used to validate the proteomic results. We additionally investigated a specific link between TMED2, TMED10, and miR-7 via a dual-luciferase reporter system, and generated CDR1as knockout cell lines via CRISPR/Cas9 editing. Results: We identified 353 proteins dysregulated upon CDR1as knockdown in 293 T cells. These CRPs were found to interact with one another and to play key roles in certain cellular pathways. Two such proteins, TMED2 and TMED10, were found to specifically contribute to the influence of CDR1as on cell proliferation. CDR1as may regulate these two TMED proteins through miR-7 sponging. We were able to further confirm these results using both CRISPRi cell lines and nude mouse models. Conclusion: This study suggested that CDR1as may regulate cell proliferation via serving as a miR-7 sponge, thereby regulating TMED2 and TMED10 expression. These results are an invaluable template for future streamlined studies of circRNAs.

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Circular RNA hsa_circ_0008344 regulates glioblastoma cell proliferation, migration, invasion, and apoptosis

Abstract: Hsa_circ_0008344 is upregulated in glioblastoma and may contribute to the progression of this malignancy.

Cited by 28 publications

References 30 publications

Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification

Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification

<p>Star Circular RNAs In Human Cancer: Progress And Perspectives</p>

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10

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