Dynamics of microbial growth and cell composition in batch culture

Wanner, Ursula; Egli, Thomas

doi:10.1111/j.1574-6968.1990.tb04084.x

Cited by 127 publications

(73 citation statements)

References 131 publications

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“…This effect is always observed after growth with sulfate (Fig. lb) and the other group 1 compounds, and was detected in the same order of magnitude by Wanner & Egli (1990) in their careful study. Whereas some of this redistribution can come from small molecules, as assayed radiochemically in E. coli (Roberts e t al., 1955) and confirmed in our microanalysis of cell material, the alteration in the level of sulfur in P. putida S-313 before and after starvation (0.45 to 0.41 %) is insufficient to account for the 20 of cell protein which is synthesized under these conditions.…”

Section: Discussionmentioning

confidence: 77%

“…It is of note that batch growth in the presence of a yield-limiting sulfur source is exponential (Figs 1-4 and Kertesz e t al., 1994b), and that growth is proportional to substrate utilization (Figs 1 and 4). It would appear that only Wanner & Egli (1990) have previously demonstrated this, for Klebsiella pneumoniae utilizing sulfate, though there is clear evidence that Cotynebacterizrrn sp. grows exponentially in a slight excess of sulfur (Omori e t al., 1992).…”

Section: Discussionmentioning

confidence: 92%

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The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313

et al. 1996

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Conditions were optimized for the batch growth of Pseudomonas putida 5-313 under sulfur-limited conditions. P. putida grew exponentially with sulfate as the sole source of sulfur, and growth was concomitant with the utilization of sulfate until it was exhausted. A further 20% of protein was synthesized after the apparent disappearance of sulfate. A mass balance for the utilized sulfate in cell material was calculated, given the observed molar growth yield of about 3.6 kg protein (mol S)-' and a sulfur content of 0.41 O/ O S in dry matter. Similar data were obtained for growth with cysteine and thiocyanate. The organism also grew exponentially with 4-toluenesulfonate (TS) as sulfur source, essentially as observed with sulfate, except that negligible protein formation after exhaustion of TS was observed. Similar data were also obtained with 4-nitrocatecholsulfate (NCS) and ethanesulfonate. Any substrate pair selected from sulfate, cysteine and thiocyanate was utilized simultaneously, and although one of the pair of substrates was always preferred, growth continued at the same rate when only one substrate remained. Growth after substrate exhaustion was observed. Any substrate pair selected from TS, NCS and ethanesulfonate gave similar data, but with less growth after exhaustion of the sulfur sources. If a mixed substrate pair was chosen from the two groups, the sulfur source from the first-named group was initially used exclusively, and the second source of sulfur was utilized subsequently, after a lag phase. The data are considered to reflect the control of scavenging for sulfur and of distribution of sulfur in the cell exerted by the sulfate-starvation-induced stimulon [Kertesz, Leisinger & Cook, J Bacferiol (1993) 175,1187

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 92%

The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313

et al. 1996

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“…We therefore developed an ecological model (Figure 4) based on previously described interactions for mutualistic systems with Monod uptake kinetics (Meyer et al, 1975;Lee et al, 1976). We modified the model to describe batch culturing and to include growth-independent fermentation by E. coli (Supplementary Table 2; Supplementary Figure 1; Wanner and Egli, 1990). Our model simulates NH 4 + excretion in a growthdependent manner because the essential carbon source for R. palustris growth (organic acids) is also the electron source for N 2 fixation.…”

Section: Resultsmentioning

confidence: 99%

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

et al. 2016

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Microbial interactions, including mutualistic nutrient exchange (cross-feeding), underpin the flow of energy and materials in all ecosystems. Metabolic exchanges are difficult to assess within natural systems. As such, the impact of exchange levels on ecosystem dynamics and function remains unclear. To assess how cross-feeding levels govern mutualism behavior, we developed a bacterial coculture amenable to both modeling and experimental manipulation. In this coculture, which resembles an anaerobic food web, fermentative Escherichia coli and photoheterotrophic Rhodopseudomonas palustris obligately cross-feed carbon (organic acids) and nitrogen (ammonium). This reciprocal exchange enforced immediate stable coexistence and coupled species growth. Genetic engineering of R. palustris to increase ammonium cross-feeding elicited increased reciprocal organic acid production from E. coli, resulting in culture acidification. Consequently, organic acid function shifted from that of a nutrient to an inhibitor, ultimately biasing species ratios and decreasing carbon transformation efficiency by the community; nonetheless, stable coexistence persisted at a new equilibrium. Thus, disrupting the symmetry of nutrient exchange can amplify alternative roles of an exchanged resource and thereby alter community function. These results have implications for our understanding of mutualistic interactions and the use of microbial consortia as biotechnology.

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“…Increase in biomass after phosphate exhaustion was recorded in all of the media (up to 40 % in the medium with glutamate), except in the one with valine. Owing to reserves of phosphate, growth can continue but at a slower rate after exhaustion of phosphate from the medium (Wanner & Egli, 1990). Phosphate storage materials can include polyphosphates, RNA or teichoic acids in Grampositive organisms (Mundry & Kuhn, 1991).…”

Section: Metabolic Transitions Linked To Nutrient Exhaustionsmentioning

confidence: 99%

Nitrogen source governs the patterns of growth and pristinamycin production in ‘Streptomyces pristinaespiralis’

Voelker¹,

Altaba²

2001

Microbiology

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Phosphate-limited synthetic culture media were designed to investigate the growth and the pristinamycin production of ' Streptomyces pristinaespiralis ' using different nitrogen sources. During balanced growth, either mineral or organic nitrogen sources were readily utilized. However, glutamate and alanine were used as both nitrogen and carbon source, sparing the utilization of the primary carbon source, glucose. Valine was utilized only for its nitrogen and consequently 2-ketoisovalerate was excreted in the medium. Ammonium prevented the utilization of nitrate. Upon phosphate limitation, glycerol, originating from the breakdown of teichoic acids, was released, allowing the recovery of phosphate from the cell wall and the continuation of growth. Under such conditions, ammonium was excreted following the consumption of glutamate and alanine and was later reassimilated after exhaustion of the primary nitrogen source. The mode of utilization of valine prevented the production of pristinamycins due to excretion of 2-ketoisovalerate, one of their direct precursors. For other nitrogen sources, pristinamycin production was controlled by nitrogen catabolic regulation linked to the residual level of ammonium. In the case of nitrate, the negative regulation was alleviated by the absence of ammonium and production then occurred precociously. In the case of amino acids and ammonium, production was delayed until after exhaustion of amino acids and depletion of ammonium.

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Dynamics of microbial growth and cell composition in batch culture

Cited by 127 publications

References 131 publications

The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313

The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

Nitrogen source governs the patterns of growth and pristinamycin production in ‘Streptomyces pristinaespiralis’

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