The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313

Abstract

Conditions were optimized for the batch growth of Pseudomonas putida 5-313 under sulfur-limited conditions. P. putida grew exponentially with sulfate as the sole source of sulfur, and growth was concomitant with the utilization of sulfate until it was exhausted. A further 20% of protein was synthesized after the apparent disappearance of sulfate. A mass balance for the utilized sulfate in cell material was calculated, given the observed molar growth yield of about 3.6 kg protein (mol S)-' and a sulfur content of 0.41 O/ O S in dry matter. Similar data were obtained for growth with cysteine and thiocyanate. The organism also grew exponentially with 4-toluenesulfonate (TS) as sulfur source, essentially as observed with sulfate, except that negligible protein formation after exhaustion of TS was observed. Similar data were also obtained with 4-nitrocatecholsulfate (NCS) and ethanesulfonate. Any substrate pair selected from sulfate, cysteine and thiocyanate was utilized simultaneously, and although one of the pair of substrates was always preferred, growth continued at the same rate when only one substrate remained. Growth after substrate exhaustion was observed. Any substrate pair selected from TS, NCS and ethanesulfonate gave similar data, but with less growth after exhaustion of the sulfur sources. If a mixed substrate pair was chosen from the two groups, the sulfur source from the first-named group was initially used exclusively, and the second source of sulfur was utilized subsequently, after a lag phase. The data are considered to reflect the control of scavenging for sulfur and of distribution of sulfur in the cell exerted by the sulfate-starvation-induced stimulon [Kertesz, Leisinger & Cook, J Bacferiol (1993) 175,1187

Assimilation of sulfur from alkyl- and arylsulfonates by Clostridium spp.

“…1E; µ = 0.21 h -1 ) displayed a different pattern, with substrate exhaustion occurring well before the end of growth [cf. Beil et al (1996)]. The overall growth yield with sulfide was approximately 3 kg protein/mol S; with X-sulfate it was approximately 4 kg protein/mol S. Growth in all cases was monophasic, similar to the curves in Fig.…”

“…Kertesz et al (1993), Beil et al (1995), Beil et al (1996)]. We thus examined whether our Clostridium spp.…”

Desulfonation of aliphatic sulfonates by Pseudomonas aeruginosa PAO

“…cysteine or thiocyanate, and derepressed during growth with other sulfur sources [ 16,18,20]. Regulation of aliphatic sulfonate desulfonation was studied in vivo in P. aeruginosa by measuring desulfonation of 4-phenylbutanesulfonate (100 PM) during growth of the cells in the presence of an excess (500 FM) of a second sulfur source (Fig.…”

Bacillus subtilis genes for the utilization of sulfur from aliphatic sulfonates

“…aromatic sulfonates and aromatic sulfate esters (Beil et al, 1995(Beil et al, , 1996. It has been shown that in E. coli, Pseudomonas putida and Pseudomonas aeruginosa, the phenomenon is due to repression by sulfate of the enzymes involved in the liberation of sulfur from organosulfur compounds (Beil et al, 1995 ;Kertesz, 1996;van der Ploeg et al, 1996).…”