Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens

Al-Ghzawi, Abd Al-Majeed; Al‐Zghoul, Mohammad Borhan; Zaitoun, Shahera; Al-Omary, Ilham Mustafa; Alahmad, Nour Alhoda

doi:10.1016/j.jtherbio.2022.103194

Cited by 10 publications

(4 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in CT max following heat shock treatment is not surprising as several studies reported significantly higher expression of heat shock proteins (HSP90 and HSP70) in heat shocked relative to untreated individuals, suggesting their role in heat tolerance (reviewed in Feder & Hofmann, 1999; King & McRae, 2015). Upregulation of heat shock proteins, which act as molecular chaperones against protein denaturation following heat stress, may explain our current results (Lopez‐Martinez & Denlinger, 2008; Al‐Ghzawi et al, 2022; Xing & Zhao, 2022). As heat shock protein levels were not measured in the current study, this warrants further investigation to fully elucidate mechanisms eliciting these plastic responses.…”

Section: Discussionsupporting

confidence: 60%

Contrasting effects of acute heat shock on physiological and ecological performance of the fall armyworm

Mbande

Mutamiswa

Nyamukondiwa

et al. 2023

Entomologia Exp Applicata

View full text Add to dashboard Cite

Temperature is a critical factor that influences the behavior, physiology, and development of ectothermic organisms. This has become even more important as acute temperature stress associated with global climate change becomes the new norm. Using the invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), we assessed its physiological and ecological responses following acute heat stress, synonymous to heat waves associated with recent climate change. Specifically, we measured the effects of short‐term exposure (for 2 h) to heat shock (at 32, 35, and 38 °C) on physiological responses, such as critical thermal minima (CTmin) and maxima (CTmax), and life‐history traits, such as reproductive success (fecundity and hatching success) and longevity, using virgin adults. Our results showed that prior acute heat shock compromised cold tolerance (CTmin) while enhancing heat tolerance (CTmax). In addition, heat shock reduced fecundity and hatching success and had dramatic effects on adult longevity. We conclude that acute heat stress associated with shifting environmental conditions may generally offset key physiological traits, affect reproduction and thus population persistence, and simultaneously have complex effects on adult lifespan.

show abstract

Section: Discussionsupporting

confidence: 60%

Contrasting effects of acute heat shock on physiological and ecological performance of the fall armyworm

Mbande

Mutamiswa

Nyamukondiwa

et al. 2023

Entomologia Exp Applicata

View full text Add to dashboard Cite

show abstract

“…However, despite that net effects were generally negative and neutral for most response variables analyzed, in some cases temperature increase can positively affect food reserves (e.g., increase honey production, advance of honey harvest days, increase hive weight, and increase in nectar volume; Gajardo‐Rojas et al., 2022; Langowska et al., 2017; Pătruică et al., 2019; Bordier et al., 2017; Gil‐Lebrero et al., 2020), reduce diseases (e.g., reduce chalkbrood, decrease deformed wing virus, increase Varroa mite fall; Nürnberger et al., 2019; Rowland et al., 2021; Bordier et al., 2017; Hillayová et al., 2022), affect positively the gene expression (e.g., improve thermo‐tolerance of workers; Al‐Ghzawi et al., 2022), increase the mitochondrial diversity (Cánovas et al., 2014), favor the geographic distribution (Castellanos‐Potenciano et al., 2017), exert positive effect on internal temperature of brood area (Gil‐Lebrero et al., 2020) and brood viability (Cebotari et al., 2019), increase glycogen levels (Bordier et al., 2017), increase the resistance of workers (i.e., daughter of heat‐stressed queens; Al‐Ghzawi et al., 2022), reduce reproduction of some pests (e.g., A. tumida ; Noor‐ul‐Ane & Jung, 2021), affect the plant‐pollinator networks (e.g., increase density, visitation rates, interactive role; Thomson, 2016; Hung et al., 2018; Cruz et al., 2022; Jaboor et al., 2022; Alzate‐Marin et al., 2021), and can increase the critical maximum temperature (Aldea‐Sánchez et al., 2021). Instead, some studies that evaluated the decrease in temperatures suggest an increase in the presence of beneficial bacteria such as Snodgrassella alvi (Castelli et al., 2022), as well as increased levels of protein, glycogen, glycerol, vitellogenin, gene expression, thus increasing the cold tolerance of honey bees (Qin et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Advances and knowledge gaps on climate change impacts on honey bees and beekeeping: A systematic review

Zapata‐Hernández,

Gajardo‐Rojas,

Calderón‐Seguel

et al. 2024

Global Change Biology

View full text Add to dashboard Cite

The Western honey bee Apis mellifera is a managed species that provides diverse hive products and contributing to wild plant pollination, as well as being a critical component of crop pollination systems worldwide. High mortality rates have been reported in different continents attributed to different factors, including pesticides, pests, diseases, and lack of floral resources. Furthermore, climate change has been identified as a potential driver negatively impacting pollinators, but it is still unclear how it could affect honey bee populations. In this context, we carried out a systematic review to synthesize the effects of climate change on honey bees and beekeeping activities. A total of 90 articles were identified, providing insight into potential impacts (negative, neutral, and positive) on honey bees and beekeeping. Interest in climate change's impact on honey bees has increased in the last decade, with studies mainly focusing on honey bee individuals, using empirical and experimental approaches, and performed at short‐spatial (<10 km) and temporal (<5 years) scales. Moreover, environmental analyses were mainly based on short‐term data (weather) and concentrated on only a few countries. Environmental variables such as temperature, precipitation, and wind were widely studied and had generalized negative effects on different biological and ecological aspects of honey bees. Food reserves, plant‐pollinator networks, mortality, gene expression, and metabolism were negatively impacted. Knowledge gaps included a lack of studies at the apiary and beekeeper level, a limited number of predictive and perception studies, poor representation of large‐spatial and mid‐term scales, a lack of climate analysis, and a poor understanding of the potential impacts of pests and diseases. Finally, climate change's impacts on global beekeeping are still an emergent issue. This is mainly due to their diverse effects on honey bees and the potential necessity of implementing adaptation measures to sustain this activity under complex environmental scenarios.

show abstract

“…Two pivotal genes, the hsp and abct , were selected for further investigation. In animals and plants such as Bacillus subtilis [ 31 ], Caenorhabditis elegans [ 32 ], A. mellifera [ 33 ], and Arabidopsis [ 34 ], a number of studies have shown that the hsp gene is a critical regulator in maintaining cell homeostasis and protecting cells from various environmental stresses [ 35 , 36 , 37 ]. Dokladny et al [ 38 ] found that the heat shock response and autophagy coordinate and undergo sequential activation and downregulation, which together maintain protein balance in cells.…”

Section: Discussionmentioning

confidence: 99%

ame-miR-34 Modulates the Larval Body Weight and Immune Response of Apis mellifera Workers to Ascosphara apis Invasion

Guo

Fan

et al. 2023

IJMS

View full text Add to dashboard Cite

MiRNAs are critical regulators of numerous physiological and pathological processes. Ascosphaera apis exclusively infects bee larvae and causes chalkbrood disease. However, the function and mechanism of miRNAs in the bee larval response to A. apis infection is poorly understood. Here, ame-miR-34, a previously predicted miRNA involved in the response of Apis mellifera larvae to A. apis invasion, was subjected to molecular validation, and overexpression and knockdown were then conducted to explore the regulatory functions of ame-miR-34 in larval body weight and immune response. Stem-loop RT-PCR and Sanger sequencing confirmed the authenticity of ame-miR-34 in the larval gut of A. mellifera. RT-qPCR results demonstrated that compared with that in the uninfected larval guts, the expression level of ame-miR-34 was significantly downregulated (p < 0.001) in the guts of A. apis-infected 4-, 5-, and 6-day-old larvae, indicative of the remarkable suppression of host ame-miR-34 due to A. apis infection. In comparison with the corresponding negative control (NC) groups, the expression level of ame-miR-34 in the larval guts in the mimic-miR-34 group was significantly upregulated (p < 0.001), while that in the inhibitor-miR-34 group was significantly downregulated (p < 0.01). Similarly, effective overexpression and knockdown of ame-miR-34 were achieved. In addition, the body weights of 5- and 6-day-old larvae were significantly increased compared with those in the mimic-NC group; the weights of 5-day-old larvae in the inhibitor-miR-34 group were significantly decreased in comparison with those in the inhibitor-NC group, while the weights of 4- and 6-day-old larvae in the inhibitor-miR-34 group were significantly increased, indicating the involvement of ame-miR-34 in modulating larval body weight. Furthermore, the expression levels of both hsp and abct in the guts of A. apis-infected 4-, 5-, and 6-day-old larvae were significantly upregulated after ame-miR-34 overexpression. In contrast, after ame-miR-34 knockdown, the expression levels of the aforementioned two key genes in the A. apis-infected 4-, 5-, and 6-day-old larval guts were significantly downregulated. Together, the results demonstrated that effective overexpression and knockdown of ame-miR-34 in both noninfected and A. apis-infected A. mellifera larval guts could be achieved by the feeding method, and ame-miR-34 exerted a regulatory function in the host immune response to A. apis invasion through positive regulation of the expression of hsp and abct. Our findings not only provide a valuable reference for the functional investigation of bee larval miRNAs but also reveal the regulatory role of ame-miR-34 in A. mellifera larval weight and immune response. Additionally, the results of this study may provide a promising molecular target for the treatment of chalkbrood disease.

show abstract

Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens

Cited by 10 publications

References 104 publications

Contrasting effects of acute heat shock on physiological and ecological performance of the fall armyworm

Contrasting effects of acute heat shock on physiological and ecological performance of the fall armyworm

Advances and knowledge gaps on climate change impacts on honey bees and beekeeping: A systematic review

ame-miR-34 Modulates the Larval Body Weight and Immune Response of Apis mellifera Workers to Ascosphara apis Invasion

Contact Info

Product

Resources

About