Dynamically Monitoring Cell Viability in a Dual‐Color Mode: Construction of an Aggregation/Monomer‐Based Probe Capable of Reversible Mitochondria‐Nucleus Migration

Abstract

Mitochondria and nucleus play crucial roles during cell apoptosis process.Inthis work, aunique fluorescent probe capable of reversible migration between mitochondria and nucleus,a sw ell as detection of cell viability in ad ual-color mode is presented. The dual-color probe targets mitochondria in healthy cells,t of orm aggregates with deep-red emission. It migrates into nucleus and binds to DNAt of orm monomers with green fluorescence during apoptosis.I nterestingly,t he migration is reversible dependent on cell viability,w hich enables the dynamic visualization of apoptosis process.W ith the probe,mitochondria and nucleus can be visualized in dual colors during apoptosis,a nd the cell viability could be monitored by the emission color and localization of the probe.

Spatiotemporally Monitoring Cell Viability through Programmable Mitochondrial Membrane Potential Transformation by Using Fluorescent Carbon Dots

“…Even little changes of mitochondrial membrane potential could result a huge influence on the cell viability and are closely connected with a series of diseases. Therefore, development of sensitive methods to monitor mitochondrial membrane potential is particularly important 5–10…”

An Activatable and Switchable Nanoaggregate Probe for Detecting H2S and Its Application in Mice Brains

“…In HSDPP , iodobenzoic acid methyl ester was selected as the reaction site due to its high selectivity and sensitivity towards H 2 S, [40] as shown in Scheme 1a. For mtDNA, the cationic quinolinium group was served as the part of binding unit, [44] which could be also anchored in the mitochondrial (Scheme 1b). In addition, the quinolinium unit could contribute to the aggregation‐induced‐emission (AIE) feature of the nanoaggregates to some extent [8] .…”

In vivo real-time tracking of tumor-specific biocatalysis in cascade nanotheranostics enables synergistic cancer treatment

“…This could be attributed to the active targeting biotin unit of BP 5 -Cy-B and its positive charge. 46,47 All these results conrmed that BP 5 -Cy-B with dual-channel uorescence response could be employed to detect different H 2 O 2 levels in living cells and increase the targeting ability of BNG.…”