Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer

Abstract

Thiol groups are important anti-oxidants and essential molecules protecting organism against the harmful effects of reactive oxygen species (ROS). The aim of our study is to evaluate thiol-disulphide homeostasis with a novel recent automated method in patients with localized prostate cancer (PC) before and six months after radical prostatectomy (RP). 18 patients with PC and 17 healthy control subjects were enrolled into the study. Blood samples were collected from the controls subjects and patients before and six months after RP. Thiol-disulphide homeostasis was determined using a recently developed novel method. Prostate-specific antigen (PSA), albumin, total protein, total thiol, native thiol, disulphide and total antioxidant status (TAS) were measured and compared between the groups. Native thiol, total thiol and TAS levels were significantly higher in the control group than the patients before RP (p < .001). There was a non-significant increase in the native thiol, total thiol and TAS levels in the patients six months after RP in comparison to the levels before RP (p values .3, .3 and .09, respectively). We found a significant negative correlation between PSA and thiol levels. Our study demonstrated that the decreased thiol and TAS levels weakened anti-oxidant defence mechanism in the patients with PC as indicated. Increased oxidative stress in prostate cancer patients may cause metabolic disturbance and have a role in the aetiopathogenesis of prostate cancer. ARTICLE HISTORY

Thiol – Disulphide Homeostasis in Polycythemia Vera

“…Additionally, they noted that the elevated GSH containing compounds is independent of the age of red blood cell 23 . Additionally, in another study demonstrated previous studies which utilized Erel and Neşelioğlu method, found that plasma thiol disulphide homeostasis was increased in degenerative diseases, such as diabetes, obesity, pneumonia, and in the case of asthma, whereas it was reduced in proliferative diseases such as multiple myeloma, renal cell carcinoma, lung cancer, and renal cancer [24][25][26][27][28][29][30] . In this study we could not find any differences between the PV and control groups in terms of the amount of plasma disulphide bonds.…”

Expression Pattern of Mitogen-inducible Gene 6 in Relation to the Extracellular Signal-regulated Kinase Pathway and Redox Status in Patients with Endometriosis

“…Thiols are organic compounds containing a sulfhydryl group (-SH) which act as physiological free radical scavengers [25]. The total plasma thiols include albumin thiols, other proteins containing free thiol groups and low-molecularweight thiols, [26] such as cysteine and glutathione [27].…”

The effect of newly initiated exercise training on dynamic thiol / disulphide homeostasis in sedentary obese adults

“…thiols, which contain a functional group of sulfhydryls, are potent antioxidant molecules that preserve the organism from the destructive influences of OS damage (Hanikoglu et al 2016). When OS develops, thiol groups in albumin, cysteine, homocysteine, glutathione, as well as other proteins are converted to disulphides.…”