In this study, samples belonging to Hydrophilidae and Helophoridae families collected from Diyarbakır were evaluated. Samples were collected between May-September 2016 and April-October 2017 among plants and mosses in shallow parts of various streams, springs, streams, deposits and ponds. In the study area, 12 taxa (species) from 7 genera belonging to Hydrophilidae family and 10 taxa (species) from 1 genus belonging to Helophoridae families were determined. Among these species, Paracymus scutellaris (Rosenhauer, 1856), Anacaena lutescens (Stephens, 1829), Helophorus discrepans (Rey, 1885), Helophorus frater (d'Ochymont, 1926) were recorded for the first time in both the Southeastern Anatolia Region and the research area. In addition, E. (L.) fuscipennis (Thomson, 1884) and Hydrobius fuscipes (Linnaeus) were recorded for the first time only from the study area.