Dunging gutters filled with fresh water in finishing barns had no effect on the prevalence of Salmonella enterica on Brazilian swine farms

Oliveira, Celso José Bruno de; Carvalho, Luis Felipe das Chagas e Silva de; Domingues, Francisco J.; Menezes, Cláudio C. P.; Fernandes, Sueli Aparecida; Tavechio, Ana Terezinha

doi:10.1016/s0167-5877(02)00099-5

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…Agona (accession no. IAL 0845/01) and S. Typhimurium (IAL 1007/01) were originally isolated from pigs [10] and kindly provided by Instituto Adolfo Lutz (Sa˜o Paulo, SP, Brazil). Both strains contained a nalidixic acid resistance marker (S. Agona nal+ and S. Typhimurium nal+ ).…”

Section: Bacteria and Inoculummentioning

confidence: 99%

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Experimental airborne transmission ofSalmonellaAgona andSalmonellaTyphimurium in weaned pigs

2005

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SUMMARYThis study tested the hypothesis of airborne transmission of Salmonella Agona (Trial I) and Salmonella Typhimurium (Trial II) in weaned pigs. The trials were performed using stainlesssteel/glass isolation cabinets connected by air ducts to permit an unidirectional airflow from cabinet 1 (two control pigs) to cabinet 3 (two sentinel pigs), passing through cabinet 2 (two inoculated pigs). Air samples, pooled faecal samples from the floor and rectal swabs were collected daily and assessed by culture and PCR. A fumigation chamber and rubber gloves coupled to the cabinets allowed sampling without opening the system. Trials I and II lasted 15 and 19 days respectively. The recovery of S. Agona and S. Typhimurium and detection of seroconversion in sentinel pigs indicate that airborne Salmonella transmission in weaned pigs over short distances is possible. Further studies on the role of aerosols in the epidemiology of Salmonella in intensive pig production should be performed.

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Section: Bacteria and Inoculummentioning

confidence: 99%

“…Both strains contained a nalidixic acid resistance marker (S. Agona nal+ and S. Typhimurium nal+ ). These serotypes were selected because they have been isolated from healthy pigs in Brazil [10] and are among the leading serotypes isolated from human sources in this country [11].…”

Section: Bacteria and Inoculummentioning

confidence: 99%

Experimental airborne transmission ofSalmonellaAgona andSalmonellaTyphimurium in weaned pigs

2005

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“…Feces samples were collected from 10 sows of a farm where no Salmonella had been isolated before (12), stored in sterile plastic bags and taken to the laboratory under refrigeration to be processed on the same day. Each sample was subdivided in two 10-gram sub-samples.…”

Section: Inoculation Of Feces Samplesmentioning

confidence: 99%

Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)

Freschi¹,

Carvalho²,

Oliveira³

2005

Braz. J. Microbiol.

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The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/ g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boilingcentrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P<0.05) when combined to Rappaport-Vassiliadis. Considering cost and efficiency parameters, we encourage the use of Müller-Kauffmann tetrathionate broth in combination with boiling-centrifugation DNA-extraction procedure.

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“…[9][10][11][12][13][14][15] The primary reservoirs for S. javiana are thought to be reptiles and amphibians that contaminate lowlying produce. 14,16 S. javiana has been isolated from domestic and farm animals, 14,17,18 from coconut, 19 and from products from India. 20 In previous outbreaks of S. javiana infection, tomatoes, 2,21 cheese, 3,4 paprika, 11 watermelon, 16 and chicken sandwiches 22 have been implicated as the source.…”

mentioning

confidence: 99%

Outbreak ofSalmonella javianaInfection at a Children's Hospital

Elward

Grim

Schroeder

et al. 2006

Infect. Control Hosp. Epidemiol.

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Objective.To determine the source of an outbreak of Salmonella javiana infection.Design.Case-control study.Participants.A total of 101 culture-confirmed cases and 540 epidemiologically linked cases were detected between May 26, 2003, and June 16, 2003, in hospital employees, patients, and visitors. Asymptomatic employees who had eaten in the hospital cafeteria between May 30 and June 4, 2003, and had had no gastroenteritis symptoms after May 1, 2003, were chosen as control subjects.Setting.A 235-bed academic tertiary care children's hospital.Results.Isolates from 100 of 101 culture-confirmed cases had identical pulsed-field gel electrophoresis patterns. A foodhandler with symptoms of gastroenteritis was the presumed index subject. In multivariate analysis, case subjects were more likely than control subjects to have consumed items from the salad bar (adjusted odds ratio [aOR], 5.3; 95% confidence interval [CI], 2.3-12.1) and to have eaten in the cafeteria on May 28 (aOR, 9.4; 95% CI, 1.8-49.5), May 30 (aOR, 3.6; 95% CI, 1.0-12.7), and/or June 3 (aOR, 4.0; 95% CI, 1.4-11.3).Conclusions.Foodhandlers who worked while they had symptoms of gastroenteritis likely contributed to the propagation of the outbreak. This large outbreak was rapidly controlled through the use of an incident command center.

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Dunging gutters filled with fresh water in finishing barns had no effect on the prevalence of Salmonella enterica on Brazilian swine farms

Cited by 8 publications

References 14 publications

Experimental airborne transmission ofSalmonellaAgona andSalmonellaTyphimurium in weaned pigs

Experimental airborne transmission ofSalmonellaAgona andSalmonellaTyphimurium in weaned pigs

Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)

Outbreak ofSalmonella javianaInfection at a Children's Hospital

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