Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2

Polarity-Specific and Pyrimidine-over-Purine Adaptive Triplex DNA Recognition by a Near-Infrared Fluorogenic Molecular Rotor

“…In addition, with their structural uniqueness, triplex DNAs have been widely explored in molecular switches, − nanodevices, − DNA crystals, catalysis, and directing chemical reactions . Practically, triplex DNAs have also been employed as sensor elements for the recognition and analysis of nucleic acids, − proteins, ,, toxic metal ions, , pH, and bioactive small molecules. ,− Furthermore, versatile chemical modifications with DNAs diversify the triplex structures with promise even in cell manipulation.…”

“…Besides characterization techniques including colorimetry, calorimetry, nuclear magnetic resonance, surface-enhanced Raman spectroscopy, electrophoresis, , mass spectrometry, circular dichroism spectroscopy, and electrochemistry, , fluorescence methods are advantageous to conveniently advance the triplex structure investigation and concomitantly imagine variant application scenarios without reconfiguration. Although covalently labeled fluorescent probes are robust in configuring the triplex switches, ,,,,,,,,− fluorophore labels themselves sometimes affect the triplex switching . Expectedly, the triplex-binding probes with label-free and fluorogenic merits are cost-effective and compatible among variant application scenarios .…”