Dual release of growth factor from nanocomposite fibrous scaffold promotes vascularisation and bone regeneration in rat critical sized calvarial defect

Kuttappan, Shruthy; Mathew, Dennis; Jo, Jun-ichiro; Tanaka, Ryusuke; Menon, Deepthy; Ishimoto, Takuya; Nakano, Takayoshi; Nair, Shantikumar V.; Nair, Manitha B.; Tabata, Yasuhiko

doi:10.1016/j.actbio.2018.07.050

Cited by 92 publications

(78 citation statements)

References 45 publications

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“…25,26 Moreover, generalized osteoporosis was reported to negatively influence implant stability and implant failures are mostly due to poor bone quality. [29][30][31] Nevertheless, native proteins which are applied are prone to degrade at a relatively rapid speed in humoral environment. [29][30][31] Nevertheless, native proteins which are applied are prone to degrade at a relatively rapid speed in humoral environment.…”

Section: Discussionmentioning

confidence: 99%

“…27,28 In recent years, scaffolds combined with specific actives substance (eg vascular endothelial growth factor and bone morphogenetic protein 2) were utilized to regulate cell behaviour in acellular therapy to promote bone formation. [29][30][31] Nevertheless, native proteins which are applied are prone to degrade at a relatively rapid speed in humoral environment. 32,33 Furthermore, HA has been F I G U R E 2 Characterization of FHA.…”

Section: Bone Disorders Such As Osteoporosis Have Become One Of Healthmentioning

confidence: 99%

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Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

et al. 2019

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Objectives: Fluorine, an organic trace element, has been shown to unfavourably effect osteoclasts function at a low dose. Use of hydroxyapatite (HA) has been effective in exploring its roles in promoting bone repair. In this study, we used HA modified with fluorine to investigate whether it could influence osteoclastic activity in vitro and ovariectomy-induced osteoclasts hyperfunction in vivo. Materials and methods: Fluorohydroxyapatite (FHA) was obtained and characterized by scanning electron microscope (SEM). Osteoclasts proliferation and apoptosis treated with FHA were assessed by MTT and TUNEL assay. SEM, F-actin, TRAP activity and bone resorption experiment were performed to determine the influence of FHA on osteoclasts differentiation and function. Moreover, HA and FHA were implanted into ovariectomized osteoporotic and sham surgery rats. Histology and Micro-CT were examined for further verification. Results: Fluorine released from FHA slowly and sustainably. FHA hampered osteoclasts proliferation, promoted osteoclasts apoptosis, suppressed osteoclasts differentiation and function. Experiments in vivo validated that FHA participation brought about an inhibitory effect on osteoclasts hyperfunction and less bone absorption. Conclusion:The results indicated that FHA served as an efficient regulator to attenuate osteoclasts formation and function and was proposed as a candidature for bone tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Section: Bone Disorders Such As Osteoporosis Have Become One Of Healthmentioning

confidence: 99%

Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

et al. 2019

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“…Among them, BMP is the most widely used osteogenic factor. BMP could induce MSC proliferation and differentiation into chondrocytes and osteoblasts [178]. In terms of heart repair, literatures reported that coinjection of MSCs and VEGF into the heart with myocardial infarction increased cell implantation and resulted in better cardiac function than either VEGF or MSC alone [136,179].…”

Section: Cell-adhesive Ligandmentioning

confidence: 99%

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Xing

Zhou

et al. 2019

Stem Cells International

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It is well known that stem cells reside within tissue engineering functional microenvironments that physically localize them and direct their stem cell fate. Recent efforts in the development of more complex and engineered scaffold technologies, together with new understanding of stem cell behavior in vitro, have provided a new impetus to study regulation and directing stem cell fate. A variety of tissue engineering technologies have been developed to regulate the fate of stem cells. Traditional methods to change the fate of stem cells are adding growth factors or some signaling pathways. In recent years, many studies have revealed that the geometrical microenvironment played an essential role in regulating the fate of stem cells, and the physical factors of scaffolds including mechanical properties, pore sizes, porosity, surface stiffness, three-dimensional structures, and mechanical stimulation may affect the fate of stem cells. Chemical factors such as cell-adhesive ligands and exogenous growth factors would also regulate the fate of stem cells. Understanding how these physical and chemical cues affect the fate of stem cells is essential for building more complex and controlled scaffolds for directing stem cell fate.

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“…The release of molecules from the scaffold can be controlled by modifying the properties and composition of the material, TABLE 1 | List of common bioactive molecules used in tissue engineering vascularization and mentioned in this review. Lee et al, 2001;Gerhardt et al, 2003;Chiu and Radisic, 2010;Chow et al, 2010;Yuen et al, 2010;Shah et al, 2011;Zheng et al, 2012;Brudno et al, 2013;Nguyen et al, 2013;Alsop et al, 2014;Lai et al, 2014;Rich et al, 2014;Stamati et al, 2014;Wu et al, 2016;LaValley et al, 2017;Turner et al, 2017;Kuttappan et al, 2018;Stejskalová et al, 2019;Wang et al, 2019FGF Chow et al, 2010Tengood et al, 2011;Lai et al, 2014;Stamati et al, 2014;Kuttappan et al, 2018;Dong et al, 2019IGF Holland et al, 2005EGF Lai et al, 2014PDGF Tengood et al, 2011Brudno et al, 2013;Lai et al, 2014;Stejskalová et al, 2019;Wang et al, 2019Glycosaminoglycans Chow et al, 2010Chow et al, 2014;Wu et al, 2016 Frontiers in Bioengineering and Biotechnology | www.frontiersin.org as well as changing the method of retaining the drug (King and Krebsbach, 2012). Boontheekul et a...…”

Section: Degradation Dependent Releasementioning

confidence: 99%

“…Similarly, Shah et al (2011) used polyelectrolyte layers to encapsulate recombinant human bone morphogenetic protein-2 (rhBMP-2) and recombinant human vascular endothelial growth factor (rhVEGF), and showed controlled release of the encapsulated factors over time both in vitro and in vivo. Furthermore, Kuttappan et al (2018) recently demonstrated the benefit of differential release by functionalizing a nanocomposite fibrous scaffold with combinations of VEGF, fibroblast growth factor (FGF) and BMP2. The release of these factors at different times led to increased tissue vascularization and bone regeneration (Kuttappan et al, 2018).…”

Section: Degradation Dependent Releasementioning

confidence: 99%

Angiogenesis in Tissue Engineering: As Nature Intended?

Mastrullo

Cathery

Velliou

et al. 2020

Front. Bioeng. Biotechnol.

129

109

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Despite the steady increase in the number of studies focusing on the development of tissue engineered constructs, solutions delivered to the clinic are still limited. Specifically, the lack of mature and functional vasculature greatly limits the size and complexity of vascular scaffold models. If tissue engineering aims to replace large portions of tissue with the intention of repairing significant defects, a more thorough understanding of the mechanisms and players regulating the angiogenic process is required in the field. This review will present the current material and technological advancements addressing the imperfect formation of mature blood vessels within tissue engineered structures.

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Dual release of growth factor from nanocomposite fibrous scaffold promotes vascularisation and bone regeneration in rat critical sized calvarial defect

Cited by 92 publications

References 45 publications

Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Angiogenesis in Tissue Engineering: As Nature Intended?

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