Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

Abstract

Objectives: Fluorine, an organic trace element, has been shown to unfavourably effect osteoclasts function at a low dose. Use of hydroxyapatite (HA) has been effective in exploring its roles in promoting bone repair. In this study, we used HA modified with fluorine to investigate whether it could influence osteoclastic activity in vitro and ovariectomy-induced osteoclasts hyperfunction in vivo. Materials and methods: Fluorohydroxyapatite (FHA) was obtained and characterized by scanning electron microscope (SEM). Osteoclasts proliferation and apoptosis treated with FHA were assessed by MTT and TUNEL assay. SEM, F-actin, TRAP activity and bone resorption experiment were performed to determine the influence of FHA on osteoclasts differentiation and function. Moreover, HA and FHA were implanted into ovariectomized osteoporotic and sham surgery rats. Histology and Micro-CT were examined for further verification. Results: Fluorine released from FHA slowly and sustainably. FHA hampered osteoclasts proliferation, promoted osteoclasts apoptosis, suppressed osteoclasts differentiation and function. Experiments in vivo validated that FHA participation brought about an inhibitory effect on osteoclasts hyperfunction and less bone absorption. Conclusion:The results indicated that FHA served as an efficient regulator to attenuate osteoclasts formation and function and was proposed as a candidature for bone tissue engineering applications.

The Effects of Calcium, Magnesium, Phosphorus, Fluoride, and Lead on Bone Tissue

“…Fluorides (F - ), the ionic form of fluorine, in trace amounts are essential for the proper development of the bones and the teeth [ 111 ]. They stimulate the proliferation of osteoblasts and inhibit the activity of osteoclasts, thus leading to an increase in bone mass [ 112 ]. Therefore, fluorine compounds are used in the treatment of osteoporosis.…”

Aucubin slows the development of osteoporosis by inhibiting osteoclast differentiation via the nuclear factor erythroid 2-related factor 2-mediated antioxidation pathway

“…TRAP is specifically distributed in the cytoplasm of osteoclasts (Liu et al. 2019 ), and during bone resorption, TRAP participates in the degradation of solid calcium phosphate substrates in the bone matrix. Type 5 TRAP has two subtypes, TRAP5a and TRAP5b (Ma et al.…”

Osteoinduction Evaluation of Fluorinated Hydroxyapatite and Tantalum Composite Coatings on Magnesium Alloys

“…Most importantly, the FHA coating possesses a nanoneedle structure which can mimic collagen fibris ( Cai et al, 2007 ; Uddin et al, 2015 ; Zhao et al, 2016 ; Shen et al, 2018 ). FHA originates from hydroxyapatite (HA), where OH- in the HA lattice is substituted with F- to form FHA ( Liu et al, 2019 ). In addition, FHA possesses excellent osteoconductivity and bioactivity.…”