Dual-Purpose Inoculants and Their Effects on Corn Silage

Abstract

This study was conducted to screen dual-purpose lactic acid bacteria (LAB) from uncontrolled farm-scale silage, and then we confirmed their effects on corn silage. The LAB were isolated from eight farm-scale corn silages, and then we screened the antifungal activity against Fusarium graminearum and the carboxylesterase activity using spectrophotometer with p-nitrophenyl octanoate as substrate and McIlvane solution as buffer. From a total of 25 isolates, 5M2 and 6M1 isolates were selected as silage inoculants because presented both activities of antifungal and carboxylesterase. According 16S rRNA gene sequencing method, 5M2 isolate had 100.0% similarity with Lactobacillus brevis, and 6M1 isolate had 99.7% similarity with L. buchneri. Corn forage was ensiled in bale silo (500 kg) for 72 d without inoculant (CON) or with mixture of selected isolates at 1:1 ratio (INO). The INO silage had higher nutrient digestibility in the rumen than CON silage. Acetate was higher and yeasts were lower in INO silage than in CON silage on the day of silo opening. In all days of aerobic exposure, yeasts were lower in INO silage than CON silage. The present study concluded that Lactobacillus brevis 5M2 and L. buchneri 6M1 confirmed antifungal and carboxylesterase activities on farm-scale corn silage.

Application of lactic acid bacteria producing antifungal substance and carboxylesterase on whole crop rice silage with different dry matter

“…Similar to the present study, several previous studies also reported the improvement of rumen digestibility by application of inoculant producing esterase [4,5]. TIn addition, the reason for the lower the fraction degradation rate in INO silages was unclear.…”

“…The WCR before and after ensiling were sub-sampled at 500 g, respectively, to analyses chemical composition and in vitro digestibility. Also, 20 g of ensiled WCR was sub-sampled and blended with 200 mL of sterile ultrapure water for 30 sec, and then filtered through two layers of cheesecloth to make silage extraction [3][4][5]. The fresh silage extraction was used to analyze pH and microbial counts.…”

“…Silage extract (first dilution) was continued in several dilutions (10 -3 [3][4][5]. Visible colonies were counted from the plates and the number of cfu was expressed per gram of silage.…”

“…The rumen microbes are capable to produce cellulase or hemicellulase, but they released limited enzymes to breakdown the lignocellulose [2]. The previous investigations revealed that several strains of lactic acid bacteria (LAB) could produce antifungal substances to inhibit the growth undesirable microbes [3][4][5] and carboxylesterase to break down the lignincellulose [1,4,5]. However, the ability of LAB to produce antifungal or carboxylesterase activities during ensiling were depended on strains and substrate sources [1,6].…”

Effects of Wormwood (Artemisia montana) Essential Oils on Digestibility, Fermentation Indices, and Microbial Diversity in the Rumen

“…The substrates and dried residues were analyzed for NDF [ 14 ] using an Ankom 200 Fiber Analyzer. Residue weights and NDF concentration were used to calculate in vitro DM digestibility (IVDMD) and in vitro NDF digestibility (IVNDFD), according to a previous study [ 15 ]. The supernatant filtered from the in vitro fermentation was used for pH, ammonia-N, and SCFA analyses.…”