Dual delivery of VEGF and ANG-1 in ischemic hearts using an injectable hydrogel

Rufaihah, Abdul Jalil; Johari, Nurul Azizah Binte; Vaibavi, Srirangam Ramanujam; Plotkin, Marian; Thien, Do Thi Di; Kofidis, Theo; Seliktar, Dror

doi:10.1016/j.actbio.2016.10.013

Cited by 91 publications

(74 citation statements)

References 36 publications

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“…Lee et al immobilized NGF into fibrin scaffolds in order to substantially improve nerve regeneration in a rat sciatic injury model (Lee et al, ). The PEG‐Fib materials have also been endowed and tested ex vivo with NGF (Berkovitch & Seliktar, ; Sarig‐Nadir & Seliktar, , ) and in vivo with other growth factors for other indications (Berdichevski, Simaan Yameen, Dafni, Neeman, & Seliktar, ; Rufaihah et al, ; Rufaihah et al, ); however, a comprehensive in vivo study of PEG‐Fib with NGF for nerve injuries is warranted as part of our future validation of this strategy.…”

Section: Resultsmentioning

confidence: 99%

Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration

Berkovitch

Cohen

Peled

et al. 2018

J Tissue Eng Regen Med

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Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.

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Section: Resultsmentioning

confidence: 99%

Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration

Berkovitch

Cohen

Peled

et al. 2018

J Tissue Eng Regen Med

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“…The myocardial thickness and the density blood vessels of the rat myocardial infarction model were larger than that of the group without treating, following the injection of a novel temperature-susceptible aliphatic polyester hydrogel (HG) crosslinked with VEGF (Wu et al, 2011). Retention of highly vascularized cardiomyocytes is a limiting factor in growth factor therapy although it presents superior performance in cardiovascular repair (Rufaihah et al, 2017). The Dex-PCL-HEMA/PNIPAAm hydrogelcon containing VEGF developed by Zhu et al (2016) and the injectable hydrogel amalgamated polyethylene glycol with fibrinogen (PEG-fibrinogen) loaded with VEGF-A designed by Rufaihah et al (2013) both are able to release and store VEGF in a controlled manner and achieve better cardiac repair than VEGF alone.…”

Section: Cell Active Factormentioning

confidence: 99%

“…The Dex-PCL-HEMA/PNIPAAm hydrogelcon containing VEGF developed by Zhu et al (2016) and the injectable hydrogel amalgamated polyethylene glycol with fibrinogen (PEG-fibrinogen) loaded with VEGF-A designed by Rufaihah et al (2013) both are able to release and store VEGF in a controlled manner and achieve better cardiac repair than VEGF alone. Moreover, in order to present a superior repair effect, an polyethylene glycol-fibrinogen (PF) hydrogels was manufactured for sustained dual transportation of VEGF and angiopoietin-1 (ANG-1) to promote myocardial therapy (Rufaihah et al, 2017). Recently, researchers' research hotspots have shifted from the development of nano-growth factor injectable hydrogels to exploring which nano-growth factor injectable hydrogel complexes present superior cardiac repair functions.…”

Section: Cell Active Factormentioning

confidence: 99%

“…Additionally, the limited function of one single GF in the delivery system was one of the restrictions. Therefore, polyethylene glycol-fibrinogen (PF) hydrogels (Rufaihah et al, 2017) was employed and incorporating with vascular endothelial growth factor (VEGF) and angiopoietin-1 (ANG-1) to achieve the effect of dual delivery of GFs in a sustained release way. Besides, other materials also play the crucial roles on cardiovascular diseases.…”

Section: The Promotion Effect Of Recovery In Cvds Via Angiogenesismentioning

confidence: 99%

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Injectable Hydrogel-Based Nanocomposites for Cardiovascular Diseases

Liao

Yang

Deng

et al. 2020

Front. Bioeng. Biotechnol.

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Cardiovascular diseases (CVDs), including a series of pathological disorders, severely affect millions of people all over the world. To address this issue, several potential therapies have been developed for treating CVDs, including injectable hydrogels as a minimally invasive method. However, the utilization of injectable hydrogel is a bit restricted recently owing to some limitations, such as transporting the therapeutic agent more accurately to the target site and prolonging their retention locally. This review focuses on the advances in injectable hydrogels for CVD, detailing the types of injectable hydrogels (natural or synthetic), especially that complexed with stem cells, cytokines, nano-chemical particles, exosomes, genetic material including DNA or RNA, etc. Moreover, we summarized the mainly prominent mechanism, based on which injectable hydrogel present excellent treating effect of cardiovascular repair. All in all, it is hopefully that injectable hydrogel-based nanocomposites would be a potential candidate through cardiac repair in CVDs treatment.

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“…[12][13][14][15] These drug releasing systems share the similar principle that drugs loaded in the inner layer are released more slowly than those in the outer layer. In addition, the drugs themselves can affect releasing dynamics due to the differences of drug structure, hydrophilia and molecular weight, [16][17][18] which consequently lead to phased drug release. As a result, the drug releasing models can be regulated to meet multiple clinical demands.…”

Section: Introductionmentioning

confidence: 99%