Dual-Channel Photoelectrochemical Ratiometric Aptasensor with up-Converting Nanocrystals Using Spatial-Resolved Technique on Homemade 3D Printed Device

Qiu, Zhenli; Shu, Jiwu; Liu, Jingfeng; Tang, Dianping

doi:10.1021/acs.analchem.8b05455

Cited by 257 publications

(131 citation statements)

References 41 publications

Supporting

Mentioning

131

Contrasting

Order By: Relevance

“…The sensor achieved a linear range of 1-12 IU/mL with a detection limit of 0.1 IU/mL, and was also tested in serum samples. Tang et al designed multiple dual (ratiometric) [70,71] and single [72] aptasensors for the detection of carcinoembryonic antigen (CEA), a broad-spectrum biomarker of pancreatic carcinoma, breast cancer and gastric carcinoma, using photo-electrochemistry. The electrode was fabricated from organic or inorganic semiconductors.…”

Section: D Printed Chip Integrated With Traditional Electrodesmentioning

confidence: 99%

See 1 more Smart Citation

3D-Printed Immunosensor Arrays for Cancer Diagnostics

Sharafeldin

Kadimisetty

Bhalerao

et al. 2020

Sensors

View full text Add to dashboard Cite

Detecting cancer at an early stage of disease progression promises better treatment outcomes and longer lifespans for cancer survivors. Research has been directed towards the development of accessible and highly sensitive cancer diagnostic tools, many of which rely on protein biomarkers and biomarker panels which are overexpressed in body fluids and associated with different types of cancer. Protein biomarker detection for point-of-care (POC) use requires the development of sensitive, noninvasive liquid biopsy cancer diagnostics that overcome the limitations and low sensitivities associated with current dependence upon imaging and invasive biopsies. Among many endeavors to produce user-friendly, semi-automated, and sensitive protein biomarker sensors, 3D printing is rapidly becoming an important contemporary tool for achieving these goals. Supported by the widely available selection of affordable desktop 3D printers and diverse printing options, 3D printing is becoming a standard tool for developing low-cost immunosensors that can also be used to make final commercial products. In the last few years, 3D printing platforms have been used to produce complex sensor devices with high resolution, tailored towards researchers’ and clinicians’ needs and limited only by their imagination. Unlike traditional subtractive manufacturing, 3D printing, also known as additive manufacturing, has drastically reduced the time of sensor and sensor array development while offering excellent sensitivity at a fraction of the cost of conventional technologies such as photolithography. In this review, we offer a comprehensive description of 3D printing techniques commonly used to develop immunosensors, arrays, and microfluidic arrays. In addition, recent applications utilizing 3D printing in immunosensors integrated with different signal transduction strategies are described. These applications include electrochemical, chemiluminescent (CL), and electrochemiluminescent (ECL) 3D-printed immunosensors. Finally, we discuss current challenges and limitations associated with available 3D printing technology and future directions of this field.

show abstract

Section: D Printed Chip Integrated With Traditional Electrodesmentioning

confidence: 99%

“…(B) Dual-Channel ratiometric photoelectrochemical detection of carcinoembryonic antigen (CEA) housed in a 3D printed device. Reproduced with permission from[70]. Copyright (2018) American Chemical Society.…”

mentioning

confidence: 99%

3D-Printed Immunosensor Arrays for Cancer Diagnostics

Sharafeldin

Kadimisetty

Bhalerao

et al. 2020

Sensors

View full text Add to dashboard Cite

show abstract

“…Point‐of‐need analytical devices have important applications in health, food, security, biological warfare and the outbreak of contagious disease. Such sensors save time, overheads and lives, and to meet this demand, a variety of technology platforms have emerged (Kulkarni et al, ; Qiu, Shu, Liu, & Tang, ).…”

Section: Introductionmentioning

confidence: 99%

Incorporating peptide aptamers into resistive pulse sensing

Maugi

Salkenova²,

Platt

2020

Med Devices & Sens

View full text Add to dashboard Cite

The use of nanocarriers within resistive pulse sensing, RPS, aids the detection and quantification of analytes. In the absence of convection, the signal strength and frequency can dependent upon the electrophoretic mobility of the nanocarrier/analyte. Here, we have developed a simple strategy to incorporate peptide aptamers onto RPS assays with enhanced electrophoretic signals. Using a hybrid DNA–Peptide nanocarrier, an existing peptide was incorporated into a rapid assay without having to engineer or modify the peptide sequence. The surface of a nanocarrier is coated with a mixture of peptide aptamers and a non‐binding DNA. The binding of the target to the peptide creates an “analyte corona” which shields the phosphate groups of the underlying DNA. This results in a change in electrophoretic mobility of the nanocarrier. The signal is concentration‐dependent and is illustrated using a peptide to a key biomarker of infection, C‐reactive protein, CRP. As a comparison, we also show the binding of the CRP to a DNA aptamer. This universal approach can be easily adapted to other peptides without the peptide itself to undergo any chemical modifications opening new opportunities and applications in RPS strategies.

show abstract

“…Thus, a series of LF assays based on immunochromatography with new labels, such as magnetic nanobeads, quantum dots and up‐converting phosphor, have attracted more and more attention nowadays [24–26]. POCT for rapid, sensitive and selective detection of molecular biomarkers have developed rapid, especially up‐converting nanoparticles and other luminescent materials as the reporter have attracted more and more researchers' interest [27–29]. Compared with the other type of LF assays, the up‐converting phosphor technology‐based lateral flow assay (UPT‐LFA) is a new emerging type of LF assays with the unique optical features of the anti‐Stokes shift and the stable fluorescence of Up‐converting phosphors (UCP) [30], which allows for the quantitative detection of pathogens by non‐technical personnel [31,32].…”

Section: Introductionmentioning

confidence: 99%

Rapid and quantitative detection of Shiga toxin1 and Shiga toxin2 based on multiple targets UPT‐LF assay

Wei

Shi

et al. 2020

Engineering in Life Sciences

View full text Add to dashboard Cite

Shiga toxin‐producing Escherichia coli (STEC) infection causes a series of diseases that are highly pathogenic and deadly in humans and animals, seriously endangering public health. Of the pathogenic factors within STEC, the two groups of Shiga toxin (Stx) consisting Stx1 and Stx2 plays a prominent role in the pathogenesis of STEC infection. In this study, we developed single‐target up‐converting phosphor technology‐based lateral flow assay (Stx‐UPT‐LFA) for the rapid detection of Stx1 and Stx2, respectively, and also developed a dual‐target Stx1/2‐UPT‐LFA based on single‐target strips to detect of Stx1 and Stx2 at the meantime within 20 min. We choose the purified Stx1 and Stx2 standard samples, and the optimum monoclonal antibody (namely 8E7‐E6, 2F6‐F8 for Stx1 and S1D8, S2C4 for Stx2) were selected for use in Stx‐UPT‐LFA in double‐antibody‐sandwich mode. The sensitivities of single‐target Stx‐UPT‐LFA for both Stx1 and Stx2 were 1 ng mL−1 with accurate quantitation ranges of 1–1000 ng mL−1 and 1–800 ng mL−1 respectively. No false‐negative result was found in the Stx2‐UPT‐LFA even with a high‐test concentration up to 1000 ng mL−1. Meanwhile, both targets detection sensitivities for dual‐target Stx1/2‐UPT‐LFA were 5 ng mL−1, and accurate quantitation ranges were 5–1000 ng mL−1 and 5–800 ng mL−1 for standard Stx1 and Stx2 solutions without cross‐interference between two targets. Both techniques showed good linearities, with a linear fitting coefficient of determination(r) of 0.9058–0.9918. Therefore, the UPT‐LFA could realize simultaneous detection for multiple targets on a single strip and thus to quickly determine the type of infectious Stxs. In addition, the single‐target Stx1‐UPT‐LFA and Stx2‐UPT‐LFA showed excellent specificity to six toxins, even at high concentrations of 1000 ng mL−1. In conclusion, the developed Stx‐UPT‐LFA allows the rapid, quantitative, reliable and simultaneous detection of Stx1 and Stx2 within 20 min, providing an alternative method for clinical diagnosis of STEC infection.

show abstract

Dual-Channel Photoelectrochemical Ratiometric Aptasensor with up-Converting Nanocrystals Using Spatial-Resolved Technique on Homemade 3D Printed Device

Cited by 257 publications

References 41 publications

3D-Printed Immunosensor Arrays for Cancer Diagnostics

3D-Printed Immunosensor Arrays for Cancer Diagnostics

Incorporating peptide aptamers into resistive pulse sensing

Rapid and quantitative detection of Shiga toxin1 and Shiga toxin2 based on multiple targets UPT‐LF assay

Contact Info

Product

Resources

About