Incorporating peptide aptamers into resistive pulse sensing

Maugi, Rhushabh; Salkenova, Zarina; Platt, Mark

doi:10.1002/mds3.10059

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…The width or full-width half-maximum relates to the velocity of the particle and the frequency of pulses relates to the concentration of nanoparticles . Studies within RPS have demonstrated how in the absence of convection the velocity can be proportional to the analyte or pore surface charge. ,, The velocity of the particle is a combination of the electroosmotic flow (EO) and electrophoretic movement under the influence of an applied electric field shown in Figure d. The velocity of nanoparticles through PU pores have been used to determine their zeta potential .…”

Section: Resultsmentioning

confidence: 99%

Multiuse Nanopore Platform with Disposable Paper Analytical Device for the Detection of Heavy Metal Ions

Heaton

Platt

2020

Ind. Eng. Chem. Res.

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The pollution of heavy metal ions within the environmental is a global problem. The rapid and precise removal of these contaminants can be aided by identifying and quantifying the composition of the sample. It is therefore crucial to develop effective portable analytical techniques to determine the levels of heavy metal contamination.Paper-based analytical devices (PADs) offer a low-cost method making them an excellent platform for onsite environmental sensors. Here we demonstrate how a PAD can be integrated into a multi-use Nanopore platform. The PAD was functionalised with different recognition ligands, who's surface charge densities varied in the presence of an analyte. The surface of the PAD was placed in contact with a Nanopore which exhibited Ion Current Rectification (ICR). The extent of ICR, was dependent upon the PAD's surface charge, and the presence of the analyte of interest i.e. the ICR phenomena was exaggerated or diminished indicating the presence of the metal ion in solution.We demonstrate the potential of PAD-ICR using a PAD functionalised with a peptide aptamer specific for nickel ions. Allowing the detection of nickel(II) as low as 0.25 μM even in the presence of other metal ions. After any measurement, the Nanopore surface can be wiped clean, and reused. PAD-ICR can also be adapted as a multiplexed sensor. This is demonstrated using a PAD with three different DNA aptamers for simultaneous and specific detection of nickel, mercury, and lead ions.

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Section: Resultsmentioning

confidence: 99%

Multiuse Nanopore Platform with Disposable Paper Analytical Device for the Detection of Heavy Metal Ions

Heaton

Platt

2020

Ind. Eng. Chem. Res.

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“…RPS has a broad range of applications including material characterisation [ 38 , 39 , 40 , 41 ], quantification of DNA/peptide analyte interactions [ 42 , 43 ], and biosensing [ 44 , 45 , 46 ]. RPS measures speeds of nanocarriers as they translocate a nanopore, and through changes in nanocarrier speed, they are able to infer carrier binding with target analyte [ 47 , 48 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

DNAzyme Sensor for the Detection of Ca2+ Using Resistive Pulse Sensing

Heaton

Platt

2020

Sensors

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DNAzymes are DNA oligonucleotides that can undergo a specific chemical reaction in the presence of a cofactor. Ribonucleases are a specific form of DNAzymes where a tertiary structure undergoes cleavage at a single ribonuclease site. The cleavage is highly specificity to co-factors, which makes them excellent sensor recognition elements. Monitoring the change in structure upon cleavage has given rise to many sensing strategies; here we present a simple and rapid method of following the reaction using resistive pulse sensors, RPS. To demonstrate this methodology, we present a sensor for Ca2+ ions in solution. A nanoparticle was functionalised with a Ca2+ DNAzyme, and it was possible to follow the cleavage and rearrangement of the DNA as the particles translocate the RPS. The binding of Ca2+ caused a conformation change in the DNAzyme, which was monitored as a change in translocation speed. A 30 min assay produced a linear response for Ca2+ between 1–9 μm, and extending the incubation time to 60 min allowed for a concentration as low as 0.3 μm. We demonstrate that the signal is specific to Ca2+ in the presence of other metal ions, and we can quantify Ca2+ in tap and pond water samples.

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“…[41][42][43] RPS measures speeds of nanocarriers as they translocate a nanopore and through changes in nanocarrier speed are able to infer carrier binding with target analyte. [44][45][46] The integration of DNAzymes onto nanocarriers presents a new method of analysis, as the cleavage of the DNAzymes could be identified and characterised. RPS has demonstrated the ability to measure speed changes between double and single stranded DNA.…”

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confidence: 99%

DNAzyme Sensor for the Detection of Metal Ions Using Resistive Pulse Sensing

Heaton¹,

Platt²

2020

Preprint

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<b>DNAzymes are DNA based catalysts that can undergo cleavage upon binding of the target analyte. The cleavage reaction is highly specific, and DNAzymes exists for a wide range of metal ions. The change of structure upon binding of a specific metal ion has given rise to many sensing strategies, but few exist with nanopore sensors. Resistive Pulse Sensing, RPS, is a platform that has emerged in recent years capable of identifying changes in DNA structure and sequence. Here we develop the use of DNAzymes with RPS technologies for the detection of Ca2+ ions in solution. Ca2+ plays an important role in biological processes, critical for cell signally, protein folding and catalysis. Extreme concentrations of Ca2+ within drinking water have also been linked to problems with corrosion, scaling and the taste of water. Using DNAzyme functionalised nanocarriers and RPS, it was possible follow the Ca2+ ions binding to the DNAzyme. The binding of Ca2+ caused a conformation change in the DNAzyme which was monitored as a change in translocation speed. By following the changes to the translocation speed, it is hypothesised that RPS can verify the changes in structure. In addition, the assay allowed the quantification of Ca2+ between 1 – 9 μM, and due its catalytic nature, increasing incubation time from 30 to 90 minutes allowed lower detection limits, down to 0.3 μM. We demonstrate that the speed changes are specific to Ca2+ in the presence of other metal ions, and we can quantify Ca2+ in tap and pond water samples.</b><br>

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Incorporating peptide aptamers into resistive pulse sensing

Cited by 4 publications

References 42 publications

Multiuse Nanopore Platform with Disposable Paper Analytical Device for the Detection of Heavy Metal Ions

Multiuse Nanopore Platform with Disposable Paper Analytical Device for the Detection of Heavy Metal Ions

DNAzyme Sensor for the Detection of Ca2+ Using Resistive Pulse Sensing

DNAzyme Sensor for the Detection of Metal Ions Using Resistive Pulse Sensing

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