Drug Purity Profiles

Grady, Lee T.; Hays, Sue E.; King, Ronald C.; Klein, Hannah R.; Mader, W.J.; Wyatt, Dorothy K.; Zimmerer, Rupert O.

doi:10.1002/jps.2600620321

Cited by 57 publications

(8 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The purities of the 4-chloroaniline and 4-chlorophenylhydroxylamine used in the incubations were established to be in excess of 99% by t.l.c. methods (Grady et al, 1973) and elemental analyses. Reference 4-chloronitrosobenzene was purified by repeated recrystallization from aqueous ethanol until it gave a single spot on t.l.c.…”

Section: Methodsmentioning

confidence: 99%

Chloroperoxidase-catalysed oxidation of 4-chloroaniline to 4-chloronitrosobenze

Corbett¹,

Chipko²,

Baden³

1978

Biochemical Journal

View full text Add to dashboard Cite

The incubation of 4-chloroaniline with chloroperoxidase and H2O2 resulted in a rapid formation of 4-chloronitrosobenzene. This enzymic oxidation displayed a pH optimum at 4.4 with a Km of 8.1x10(-4)M and catalytic-centre activity of 312. The initial rate of the reaction was strongly affected by the presence of halide ions. 4-Chlorophenylhydroxylamine was even more rapidly converted into the nitroso compound. A reaction mechanism is proposed on the basis of currently accepted theory for the catalytic action of chloroperoxidae. A noteworthy aspect of this new reaction is the difference in the products previously reported for the action of classical peroxidases on anilines and the single nitroso product resulting from chloroperoxidase oxidation.

show abstract

Section: Methodsmentioning

confidence: 99%

Chloroperoxidase-catalysed oxidation of 4-chloroaniline to 4-chloronitrosobenze

Corbett¹,

Chipko²,

Baden³

1978

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…Physical constants and purity profiles of drugs have been determined by using differential scanning calorimetry. [ 14 135 ] The latter can be assessed by the melting behavior observed in the recorded thermograms. Peak integration, according to the van’t Hoff relationship, and T m values are used for batch-to-batch consistency and to test for impurities that will change the melting profile.…”

Section: Drug Puritymentioning

confidence: 99%

“…Peak integration, according to the van’t Hoff relationship, and T m values are used for batch-to-batch consistency and to test for impurities that will change the melting profile. [ 135 ] Although it is difficult to quantitatively measure the percentage or type of impurities, DSC provides a quick and reliable means of establishing batch variability and is a qualitative screen for contamination. [ 14 135 136 ] Nevertheless multiple techniques will be necessary to allow proper quantitative analysis.…”

Section: Drug Puritymentioning

confidence: 99%

“…[ 135 ] Although it is difficult to quantitatively measure the percentage or type of impurities, DSC provides a quick and reliable means of establishing batch variability and is a qualitative screen for contamination. [ 14 135 136 ] Nevertheless multiple techniques will be necessary to allow proper quantitative analysis. [ 135 136 ]…”

Section: Drug Puritymentioning

confidence: 99%

“…[ 14 135 136 ] Nevertheless multiple techniques will be necessary to allow proper quantitative analysis. [ 135 136 ]…”

Section: Drug Puritymentioning

confidence: 99%

See 2 more Smart Citations

Differential scanning calorimetry: An invaluable tool for a detailed thermodynamic characterization of macromolecules and their interactions

2011

View full text Add to dashboard Cite

Differential Scanning Calorimetry (DSC) is a highly sensitive technique to study the thermotropic properties of many different biological macromolecules and extracts. Since its early development, DSC has been applied to the pharmaceutical field with excipient studies and DNA drugs. In recent times, more attention has been applied to lipid-based drug delivery systems and drug interactions with biomimetic membranes. Highly reproducible phase transitions have been used to determine values, such as, the type of binding interaction, purity, stability, and release from a drug delivery mechanism. This review focuses on the use of DSC for biochemical and pharmaceutical applications.

show abstract