Differential scanning calorimetry: An invaluable tool for a detailed thermodynamic characterization of macromolecules and their interactions

Abstract

Differential Scanning Calorimetry (DSC) is a highly sensitive technique to study the thermotropic properties of many different biological macromolecules and extracts. Since its early development, DSC has been applied to the pharmaceutical field with excipient studies and DNA drugs. In recent times, more attention has been applied to lipid-based drug delivery systems and drug interactions with biomimetic membranes. Highly reproducible phase transitions have been used to determine values, such as, the type of binding interaction, purity, stability, and release from a drug delivery mechanism. This review focuses on the use of DSC for biochemical and pharmaceutical applications.

Synthesis and evaluation of anti-oxidant and cytotoxic activities of novel 10-undecenoic acid methyl ester based lipoconjugates of phenolic acids

“…DSC is a sensitive technique and has been used for investigating the thermotropic properties of several compounds including biological macromolecules, drugs and lipid-based materials for their stability and other characteristics [16]. In the present study, pure linoleic acid and linoleic acid containing the synthesized compounds were subjected to DSC analysis.…”

Effect of drying methods on physico-chemical and functional properties of chickpea protein concentrates

“…The choice of solid state of proteins to evaluate the thermal properties was because the chemical and physical degradation is significantly reduced in this form (Chiu and Prenner, 2011). Moreover, we can follow the stability of powder during the storage conditions and predict its thermal behaviour in food system with low content in water such as pharmaceutical product, extruded product and sesame-based confectionery product and food improvers containing chickpea proteins.…”

Experimental techniques involved in the development of oral insulin carriers

“…DSC is a thermodynamic technique, which measures heat capacity as a function of temperature [86]. The signal from a sample cell is compared with a reference cell lacking protein in an identical solution environment.…”