Drug-induced anti-inflammatory response in A549 cells, as detected by Raman spectroscopy: a comparative analysis of the actions of dexamethasone and <i>p</i>-coumaric acid

Silva, Elaine Cristina Oliveira da; Santos, Flávio Martins dos; Ribeiro, Ana Rúbia Batista; Souza, Samuel T.; Barreto, Emiliano; Fonseca, E. J. S.

doi:10.1039/c8an01887a

Cited by 17 publications

(10 citation statements)

References 37 publications

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“…In turn, p-coumaric acid has been shown to decrease the production of IL-8 in cigarette smoke extract (CSE)stimulated A549 cells [65], and ferulic acid treatment was shown to suppress one of the main features of airway remodeling, indicated by reduction of mucus production [66], and to present anti-viral properties against RSV and influenza [67][68][69]. Therefore, it is possible that the cumulative action these substances may enable complementary remodeling treatment.…”

Section: Discussionmentioning

confidence: 99%

Leonurus sibiricus root extracts decrease airway remodeling markers expression in fibroblasts

Wieczfińska

Sitarek

Kowalczyk

et al. 2020

Clinical and Experimental Immunology

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Airway remodeling is a complex process, and controlling it appears to be a key for halting the progression of asthma and other obstructive lung diseases. The aim of this study was to evaluate the anti‐remodeling properties of extracts from transformed and transgenic L. sibiricus roots with overexpression of AtPAP1 transcriptional factor. Our study shows for the first time that transformed AtPAP1 TR extract from L. sibiricus root may affect the remodeling process.

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Section: Discussionmentioning

confidence: 99%

Leonurus sibiricus root extracts decrease airway remodeling markers expression in fibroblasts

Wieczfińska

Sitarek

Kowalczyk

et al. 2020

Clinical and Experimental Immunology

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“…The MTT assay was used to evaluate how uvaol affected cell viability [49]. In brief, NIH3T3 fibroblasts (7 × 10 3 cells/well) and tEnd.1 cells (10 4 cells/well) were seeded in 96-well plates and treated with uvaol at concentrations of 1, 10, 25, 50, and 100 µM for 24 h. Thereafter, the medium was replaced with fresh medium containing 5 mg/mL MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide).…”

Section: Cell Viability Assaymentioning

confidence: 99%

Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice

et al. 2020

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Uvaol is a natural pentacyclic triterpene that is widely found in olives and virgin olive oil, exerting various pharmacological properties. However, information remains limited about how it affects fibroblasts and endothelial cells in events associated with wound healing. Here, we report the effect of uvaol in the in vitro and in vivo healing process. We show the positive effects of uvaol on migration of fibroblasts and endothelial cells in the scratch assay. Protein synthesis of fibronectin and laminin (but not collagen type I) was improved in uvaol-treated fibroblasts. In comparison, tube formation by endothelial cells was enhanced after uvaol treatment. Mechanistically, the effects of uvaol on cell migration involved the PKA and p38-MAPK signaling pathway in endothelial cells but not in fibroblasts. Thus, the uvaol-induced migratory response was dependent on the PKA pathway. Finally, topical treatment with uvaol caused wounds to close faster than in the control treatment using experimental cutaneous wounds model in mice. In conclusion, uvaol positively affects the behavior of fibroblasts and endothelial cells, potentially promoting cutaneous healing.

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“…3 An inflammatory study at cultural circumstances exhibited that treatment with dexamethasone reduces the secretion of interleukin IL-8. 4 DXP and other derivatives of the glucocorticoid hydrocortisone family have been prohibited as per their abusing as doping drugs in sports to boost performance. 5 Consequently, it is significant to improve a sensitive analytical approach to determine of DXP.…”

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confidence: 99%

Development of a Simple Colorimetric Determination of Dexamethasone

Al-Janabi¹,

Mahmood²,

Luaibi³

2020

ijddt

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A simple, environmentally benign, cost-effective, and sensitive colorimetric determination for the pharmaceutical drug dexamethasone sodium phosphate (DXP) has been developed by the formation of a colored complex with fluoranil. The process was sensitive and linear over the range 1 to 40 μg/mL, excellent correlation coefficient 0.9989, recovery% 99.80 ± 1.3, limit of detection (LOD) and limit of quantification (LOQ) were 0.23 and 0.9 μg/mL, respectively, and good RSD ~1.63%. The experimental conditions were optimized after an intensive study. The approach was validated statistically for the quantification of the analyte in its pure and/or pharmaceutical form. Despite the proposed approach is selective, it still can be applied for the analysis of other pharmaceuticals.

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Drug-induced anti-inflammatory response in A549 cells, as detected by Raman spectroscopy: a comparative analysis of the actions of dexamethasone and p-coumaric acid

Abstract: p-Coumaric acid, a natural phenolic compound found throughout nature, has been presented as an alternative for the treatment of inflammatory diseases.

Cited by 17 publications

References 37 publications

Leonurus sibiricus root extracts decrease airway remodeling markers expression in fibroblasts

Leonurus sibiricus root extracts decrease airway remodeling markers expression in fibroblasts

Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice

Development of a Simple Colorimetric Determination of Dexamethasone

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