Development of a Simple Colorimetric Determination of Dexamethasone

Abstract

A simple, environmentally benign, cost-effective, and sensitive colorimetric determination for the pharmaceutical drug dexamethasone sodium phosphate (DXP) has been developed by the formation of a colored complex with fluoranil. The process was sensitive and linear over the range 1 to 40 μg/mL, excellent correlation coefficient 0.9989, recovery% 99.80 ± 1.3, limit of detection (LOD) and limit of quantification (LOQ) were 0.23 and 0.9 μg/mL, respectively, and good RSD ~1.63%. The experimental conditions were optimized after an intensive study. The approach was validated statistically for the quantification of the analyte in its pure and/or pharmaceutical form. Despite the proposed approach is selective, it still can be applied for the analysis of other pharmaceuticals.

Simple Analytical Method on the Determination of Dexamethasone in Herbal Medicine

“…e measurement results show the maximum readable dexamethasone at a wavelength of 483 nm. is is close to the following literature which shows that the reaction of dexamethasone-hydroxylamine complex and fluoranyl has a maximum wavelength of 485 nm [12].…”

“…e results of the percent recovery in Table 1 showed that the best conditions were when the addition of hydroxylamine hydrochloride was 20 mg and the best reflux time was 60 minutes. It is because of the less reflux time used, the complex reaction between dexamethasone and hydroxylamine has not been maximally formed and there are still residual substances that have not reacted, but on the other hand, if the reflux time is too long, the complex reaction that has been formed will slowly be degraded [12,17].…”

“…Analysis of dexamethasone in herbal medicine samples was carried out by UV-Vis spectrophotometry as the research method and HPLC as the standard method. e reaction that occurs between the dexamethasone-hydroxylamine complex and fluoranyl indicates that the carbonyl group of dexamethasone which has been modified into a dexamethasone-hydroxylamine complex will contain a weak acid hydroxyl group so that it easily reacts with fluoranyl which then causes the formation of a complex color that can be detected by UV-Vis spectrophotometry [12]. It happens because fluoranyl is a strong π (phi) acceptor.…”

“…One of them is by using a spectrophotometric approach. Al-Janabi et al developed a colorimetric determination of dexamethasone in tablets using UV-Vis spectrophotometry [12]. ey used uoranyl as a reagent to detect dexamethasone-hydroxylamine complex, and it was measured by using UV-Vis spectrophotometry.…”