Droplet microfluidics: A tool for biology, chemistry and nanotechnology

Mashaghi, Samaneh; Abbaspourrad, Alireza; Weitz, David A.; Oijen, Antoine M. van

doi:10.1016/j.trac.2016.05.019

Cited by 313 publications

(193 citation statements)

References 85 publications

(80 reference statements)

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“…By controlling the concentration of molecules in the sample, the number encapsulated in each droplet can be tuned down to single molecules24. Critical to using double emulsions for PCR detection of target molecules is stabilizing the droplets through thermal cycling reactions.…”

Section: Resultsmentioning

confidence: 99%

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Sukovich

Lance

Abate

2017

Sci Rep

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Genetic heterogeneity is an important feature of many biological systems, but introduces technical challenges to their characterization. Even with the best modern instruments, only a small fraction of DNA molecules present in a sample can be read, and they are recovered in the form of short, hundred-base reads. In this paper, we introduce 3dPCR, a method to sort DNA molecules with sequence specificity. 3dPCR allows heterogeneous populations of DNA to be sorted to recover long targets for deep sequencing. It is valuable whenever a target sequence is rare in a mixed population, such as for characterizing mutations in heterogeneous cancer cell populations or identifying cells containing a specific genetic sequence or infected with a target virus.

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Section: Resultsmentioning

confidence: 99%

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Sukovich

Lance

Abate

2017

Sci Rep

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“…It has a powerful potential to support scientific progress in further analysis of the cells. Interested readers can refer to the themed reviews 165,166 for more information.…”

Section: F Dropletsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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“…As a platform, PDMS-based microfluidic devices are used for a wide range of applications including immunoassays, separation of proteins and DNA, and the sorting and manipulation of living cells allowing for researchers to gain insights into cell biology [4]. However, microfluidic systems are not limited to biological research, as they have been used by nanotechnology researchers as a high-throughput way of producing nanodevices [5] and as a platform for many analytical chemistry techniques such as electrophoresis and chromatography [6]. …”

Section: Introductionmentioning

confidence: 99%

Low-cost feedback-controlled syringe pressure pumps for microfluidics applications

2017

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Microfluidics are widely used in research ranging from bioengineering and biomedical disciplines to chemistry and nanotechnology. As such, there are a large number of options for the devices used to drive and control flow through microfluidic channels. Commercially available syringe pumps are probably the most commonly used instruments for this purpose, but are relatively high-cost and have inherent limitations due to their flow profiles when they are run open-loop. Here, we present a low-cost ($110) syringe pressure pump that uses feedback control to regulate the pressure into microfluidic chips. Using an open-source microcontroller board (Arduino), we demonstrate an easily operated and programmable syringe pump that can be run using either a PID or bang-bang control method. Through feedback control of the pressure at the inlets of two microfluidic geometries, we have shown stability of our device to within ±1% of the set point using a PID control method and within ±5% of the set point using a bang-bang control method with response times of less than 1 second. This device offers a low-cost option to drive and control well-regulated pressure-driven flow through microfluidic chips.

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Droplet microfluidics: A tool for biology, chemistry and nanotechnology

Cited by 313 publications

References 85 publications

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Low-cost feedback-controlled syringe pressure pumps for microfluidics applications

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