Dried extracts of Encholirium spectabile (Bromeliaceae) present antioxidant and photoprotective activities in vitro

Abstract

The antioxidant and photoprotective activities of dried extracts from the leaves of Encholirium spectabile were investigated. It was also evaluated the total phenolic and flavonoid contents by the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using of 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching and compared with ascorbic acid, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) used as reference compounds. The photoprotective effect was evaluated by the spectrophotometric method. The most significant total phenolic and flavonoid contents was of 188.50 ± 27.50 mg of gallic acid equivalent/g and 129.70 ± 4.59 mg of catechin equivalent/g, respectively, for chloroform fraction (Es-CHCl3). The Es-CHCl3 also presented the best antioxidant activity (IC50 25.35 ± 4.35 μg/ml) for DPPH scavenging. The ethanol extract (Es-EtOH), Es-CHCl3 and the fraction ethyl acetate (Es-AcOEt) showed characteristic absorption bands in regions UVB and UVA in a concentration-dependent manner. Es-CHCl3 presented the highest sun protection factor SPF (8.89 ± 2.11). It shows the possibility to use this extract as sunscreen in pharmaceutical preparations.

Evaluation of Photoprotective Potential and Percutaneous Penetration by Photoacoustic Spectroscopy of the Schinus terebinthifolius Raddi Extract

“…Structure elucidation. The structures of purified compounds were identified by comparing their NMR data with the literature (20,21). Nuclear magnetic resonance spectra were recorded with a Bruker DRX-400 spectrometer at 300 MHz ( 1 H, COSY) and 75.5 MHz ( 13 C, DEPT) using DMSO-d 6 as the solvent.…”

“…Ethyl gallate was also found in the extract of the aerial parts of this plant (35). Previous studies attributed strong antioxidant activity to gallic acid and ethyl gallate, phenolic compounds that were present in the extract (20,36,37).…”

“…Previous phytochemical studies reported the isolation of flavonoids and phenolic compounds from extracts of Schinus terebinthifolius (17,18). These compounds are known to absorb UV radiation and the phenolic one has been associated with antioxidant activities (19,20).…”

Accelerated Aging as a Vigor Test in Encholirium spectabile Seeds

“…& Schult. f., a native species belonging to the Bromeliaceae family, subfamily Pitcairnioideae, is popularly known in Brazil as "macambira-de-flecha" (Oliveira et al, 2013).…”

A Green Antioxidant Activity‐Integrated Dual‐Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On‐Line DPPH‐CE‐DAD

“…1, 1-Diphenyl-2-picrylhydrazyl (DPPH) which is a radical-containing compound is usually used to quantify the antioxidant activity of various samples [13, 14]. Various methods were used for the assessment of antioxidants in complex samples, such as the DPPH radical with a spectrophotometer, dot-blot test on a thin-layer chromatography (TLC) plate, and on-line HPLC-DPPH assay [14–16].…”