A Green Antioxidant Activity‐Integrated Dual‐Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On‐Line DPPH‐CE‐DAD

Li, Jin; Liu, Jiao; Liu, Wei; Yu, Xie‐an; Cao, Jun; An, Malaviya; Gao, Xiumei; Chang, Yan‐xu

doi:10.1155/2016/2712476

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…Our analysis predicted TNF, IL-6, IFNγ, and IL-1β are among the top 10 important nodes in the aGVHD molecular network (genecards.com). Network pharmacology analysis and literature search revealed that 38 compounds detected in XBJ ingredients may benefit patients receiving allo-HSCT therapy and those suffering from aGVHD, including active compounds in XBJ that were detected in serum such as paeoniflorin and carthamin yellow (Huang et al, 2011; Li et al, 2016a; Zuo et al, 2017a,b). Our analysis suggested XBJ may prevent aGVHD at different phases.…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology-Guided Development of a Novel Integrative Regimen to Prevent Acute Graft-vs.-Host Disease

et al. 2018

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Lapses in the graft-vs.-host disease (GVHD) prophylaxis and side effects of current standard care following allogeneic hematopoietic stem cell transplantation (allo-HSCT) call for novel regimens. Traditional approaches targeting T cells showed limited success in preventing acute GVHD (aGVHD). System medicine showed promising results treating complex diseases such as sepsis and multi-organ dysfunction syndrome (MODS). Adapting established network pharmacology analysis methods, we aimed to develop novel integrative regimens to prevent aGVHD. Our network pharmacology analysis predicted that Xuebijing injection (XBJ) targets a series of key node proteins in aGVHD network. It also unveiled that Salviae miltiorrhizae (Danshen), an herb in Xuebijing formula, which prevented aGVHD in rats, shares five out of six key GVHD node proteins targeted by XBJ. Interestingly, network pharmacology analysis indicated Xuebijing may share multiple aGVHD targets with Cyclosporin A (CsA), a first-line drug for preventing aGVHD in the clinic. Based on current information, we hypothesized that combination of XBJ and CsA may yield superior results in aGVHD prevention than either drug alone. We performed in vitro and in vivo assays to validate the predictions by the network pharmacology analysis. In vitro assays revealed XBJ prevented platelet aggregation and NF-κB nuclear translocation in macrophages. XBJ also promoted angiogenesis in tube-formation assay. Importantly, the combination of CsA and XBJ was effective in rescuing mice subjected to lethal GVHD. XBJ contributed to the rescue through preventing NF-κB nuclear translocation, attenuating inflammation and maintaining viability of macrophages. Overall, network pharmacology is a powerful tool to develop novel integrative regimens. Combination of XBJ and CsA may shed light on preventing aGVHD.

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Section: Discussionmentioning

confidence: 99%

Network Pharmacology-Guided Development of a Novel Integrative Regimen to Prevent Acute Graft-vs.-Host Disease

et al. 2018

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“…These factors were the main ones influencing investigation. In several papers on study of antioxidant activity using CE and DPPH published before 24 – 26 , the optimized parameters were similar to the ones described here. Hence, the parameters chose to explore for the optimum performance were comprehensive and necessary.…”

Section: Resultsmentioning

confidence: 99%

A powerful on line ABTS+–CE-DAD method to screen and quantify major antioxidants for quality control of Shuxuening Injection

et al. 2018

Sci Rep

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A novel method of on-line 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)-Capillary Electrophoresis-Diode Array Detector (on-line ABTS+-CE-DAD) was developed to screen the major antioxidants from complex herbal medicines. ABTS+, one of well-known oxygen free radicals was firstly integrated into the capillary. For simultaneously detecting and separating ABTS+ and chemical components of herb medicines, some conditions were optimized. The on-line ABTS+-CE-DAD method has successfully been used to screen the main antioxidants from Shuxuening injection (SI), an herbal medicines injection. Under the optimum conditions, nine ingredients of SI including clitorin, rutin, isoquercitrin, Quercetin-3-O-D-glucosyl]-(1-2)-L-rhamnoside, kaempferol-3-O-rutinoside, kaempferol-7-O-β-D-glucopyranoside, apigenin-7-O-Glucoside, quercetin-3-O-[2-O-(6-O-p-hydroxyl-E-coumaroyl)-D-glucosyl]-(1-2)-L-rhamnoside, 3-O-{2-O-[6-O-(p-hydroxyl-E-coumaroyl)-glucosyl]}-(1-2) rhamnosyl kaempfero were separated and identified as the major antioxidants. There is a linear relationship between the total amount of major antioxidants and total antioxidative activity of SI with a linear correlation coefficient of 0.9456. All the Relative standard deviations of recovery, precision and stability were below 7.5%. Based on these results, these nine ingredients could be selected as combinatorial markers to evaluate quality control of SI. It was concluded that on-line ABTS+-CE-DAD method was a simple, reliable and powerful tool to screen and quantify active ingredients for evaluating quality of herbal medicines.

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“…The capillary electrophoresis (CE) has the advantages of low solvent consumption and high efficiency, so it was widely applied in screening enzyme inhibitors, complex samples analysis, and derivatization [18,19,20] in recent years. At present, the in-capillary ABTS + -CE-DAD method [21] and DPPH-CE-DAD method [22,23] were established to evaluate the antioxidant activity of TCM. Based on these methods, an in-capillary [Fe(ferrozine) 3 ] 2+ -CE-DAD was proposed to evaluate the ferrous ions chelating ability of TCM.…”

Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)3]2+-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae)

Liu

Wang

et al. 2019

Molecules

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An efficient microwave-assisted extraction (MAE) combined with in-capillary [Fe(ferrozine)3]2+-capillary electrophoresis-Diode Array Detector (in-capillary [Fe(ferrozine)3]2+-CE-DAD) was developed to screen active components with the ability to chelate ferrous ions and determine the total antioxidant activity. The MAE conditions, including methanol concentration, extraction power, extraction time, and the ratio of material to liquid, were optimized by an L9(34) orthogonal experiment. Background buffer, voltage, and cartridge temperature that affect the separation of six compounds were optimized. It was found that rutin and quercetin were the main components chelating ferrous ions in Flos Sophorae Immaturus (Flos Sophorae) by the in-capillary [Fe(ferrozine)3]2+-CE-DAD. The recoveries were ranged from 95.2% to 104%. It was concluded that the MAE combined with in-capillary [Fe(ferrozine)3]2+-CE-DAD method was a simple, reliable, and efficient tool for screening active components from the complex traditional Chinese medicine samples and evaluating their ability to chelate ferrous ions.

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A Green Antioxidant Activity‐Integrated Dual‐Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On‐Line DPPH‐CE‐DAD

Cited by 6 publications

References 21 publications

Network Pharmacology-Guided Development of a Novel Integrative Regimen to Prevent Acute Graft-vs.-Host Disease

Network Pharmacology-Guided Development of a Novel Integrative Regimen to Prevent Acute Graft-vs.-Host Disease

A powerful on line ABTS+–CE-DAD method to screen and quantify major antioxidants for quality control of Shuxuening Injection

Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)3]2+-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae)

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