Dried blood spot liquid chromatography assay for therapeutic drug monitoring of metformin

Study of dried blood spots technique for the determination of dextromethorphan and its metabolite dextrorphan in human whole blood by LC–MS/MS

“…A wide range of compounds have been analyzed from DBS, spanning a molecular weight range from amino acids to hormones and RNAs [2]. It has been reported that the DBS assay has been successfully applied for the analysis of several drugs such as acetaminophen, rifampicin, tacrolimus, antimalarials, antiepileptics, antiretrovirals, metformin, and paracetamols [1,[4][5][6][7][8][9][10]. Most of the reported DBS methods employed high performance liquid chromatography (HPLC) separation followed by ultraviolet (UV) detection.…”

Multi-component plasma quantitation of anti-hyperglycemic pharmaceutical compounds using liquid chromatography–tandem mass spectrometry

“…Available reports suggest that liquid chromatography-mass spectrometry (LC-MS) and LC with UV detection in conjunction with solid phase extraction (SPE) or liquid-liquid extraction (LLE) are the most commonly used methods for detection and quantitation of anti-diabetic drugs in biological samples [16][17][18][19][20][21][22].…”

Analysis of metformin, sitagliptin and creatinine in human dried blood spots

“…Those metformin levels would add up to mean values of 1.3 g/mL (n = 58) or 2.0 g/mL (n = 6) [13,14]. The maximum metformin concentration in a DBS of a healthy volunteer taking a single dose of 850 mg was approximately 1.6 g/mL [17]. These results are consistent with the metformin concentrations we found in DBS of the diabetic patients (0.61 and 1.27 g/mL) and it suggests that our method is suitable for pharmacokinetic evaluations.…”

“…The LLOQ, defined as the lowest concentrations that could be determined with a precision displaying a relative standard deviation of ≤20 %, an accuracy between 80 and 120% and a response five times higher than the blank sample, was 0.2 g/mL (1.55 M) for metformin, Previously a HPLC method for analysis of metformin from human DBS with a LLOQ of 0.15 g/mL was described [17]. An approach employing laser diode thermal desorption tandem mass spectrometry for the analysis of metformin and sitagliptin in human DBS had a LLOQ of 5 ng/mL for both compounds [18].…”

“…Analysis of metformin from DBS has been accomplished using HPLC-UV [17] and both metformin and sitagliptin were quantified in DBS employing LC-MS/MS [18]. In clinical chemistry labs creatinine is routinely determined using colorimetric assays, either the Jaffé method using alkaline picrate or an enzymatic assay yielding a color product [9].…”