Multi-component plasma quantitation of anti-hyperglycemic pharmaceutical compounds using liquid chromatography–tandem mass spectrometry

Determination of metformin in human plasma using hydrophilic interaction liquid chromatography–tandem mass spectrometry

“…To our knowledge, the HILIC-MS/MS method described has a higher sensitivity than previously published procedures [8][9][10][11][12][13][14][15][16][17][18]23,26]. The combination of HILIC chromatography and electrospray ionization MS detection offers an increase in sensitivity over reversed phase chromatography because of the higher concentration of organic mobile phase which is more volatile and provides greater ionization efficiency for metformin.…”

“…Various analytical techniques have been employed to solve this problem, including gas chromatography (GC) with derivatization [24], capillary electrophoresis (CE) [25] and high performance liquid chromatography (HPLC) [17]. HPLC (normal phase, reversed phase, cation exchange, ion pair and HILIC) with UV or mass spectrometric detection was the most commonly used method for metformin analysis [8][9][10][11][12][13][14][15][16][17][18]23]. However, these methods suffered from a number of disadvantages, such as low sensitivity, complex and time-consuming preparation procedures, the need for large sample volumes, and long chromatographic run times.…”

“…Various analytical strategies have been used for metformin measurement including normal phase chromatography (silica and cyano) [8][9][10], cation exchange chromatography [11,12], ion pair chromatography [13,14] and reversed phase chromatography [15][16][17][18] with UV or mass spectrometric detection. However, most of these methods exhibited low sensitivity with the lower limit of quantification (LLOQ) ranging from 7.8 to 30 ng/mL [8][9][10][11][12]14,[16][17][18], required large sample volumes [8,[11][12][13][14][16][17][18], used complex sample preparation procedures [9,11,13], and required long chromatographic run time [8,9,12,13,17].…”

Fragmentation of toxicologically relevant drugs in positive‐ion liquid chromatography–tandem mass spectrometry

“…MS-MS data for two classes anti-diabetic drugs are discussed here: (1) secretagogues, including sulfonylureas and meglitinides, and (2) sensitizers, including biguanides and thiazolidinediones. Multiresidue analysis of anti-diabetic agents has been described for analysis in plasma (Maurer et al, 2002;Wang & Miksa, 2007) and in equine urine . First-generation sulfonylurea anti-diabetic agents include compounds like carbutamide, tolazamide, and tolbutamide.…”

Development and validation of a liquid chromatography/tandem mass spectrometry assay for the simultaneous determination of nateglinide, cilostazol and its active metabolite 3,4-dehydro-cilostazol in Wistar rat plasma and its application to pharmacokinetic study

“…There are more prescriptions of using NTG and CLZ in combination in diabetics and no such information on the drug-drug interaction effect of these two drugs when given together. While investigating the analytical methods in this regard, we found several individual methods for estimating these drugs alone in biological fluids by reverse phase chromatographic methods [11][12][13][14][15][16][17][18][19][20]. All these individual methods suffer from long analysis time, low sensitivity and difficult extraction procedures.…”