Draft Genome Sequences of Eight Canadian Citrobacter braakii and Citrobacter freundii Strains

Duceppe, Marc‐Olivier; Phipps-Todd, Beverley; Carrillo, Catherine D.; Huang, Hongsheng

doi:10.1128/mra.00273-19

Cited by 10 publications

(9 citation statements)

References 11 publications

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“…In addition, for the samples used in the current study, live coliforms, generic E. coli and Salmonella were detected for the biosolids collected in 2009 as described in a separate publication for which the WWTPs were named as WWTP A and C (Yergeau et al, 2016;Duceppe et al, 2019). Similar results for coliforms and generic E. coli have been obtained for the samples used in 2019 with the WWTPs referred as WWTP A and B respectively) (data not shown).…”

Section: Study Sites and Sample Collectionsupporting

confidence: 79%

“…Several studies have shown the presence of live bacteria and ARBs in biosolids, indicating that treatments in WWTPs cannot completely inactivate all the bacteria (Alam et al, 2007;Viau and Peccia, 2009b;Yergeau et al, 2016). For the samples used in the current study, live coliforms, generic E. coli and Salmonella were detected for the biosolid samples collected in 2009 as described in separate publications for which the WWTPs were named as WWTP A and C (Yergeau et al, 2016;Duceppe et al, 2019). Similar results for coliform and generic E. coli have been obtained for the samples used in 2019 with the WWTPs referred as WWTP-A and -B respectively) (data not shown).…”

Section: Co-occurrence Patterns Between Args and Bacterial Communitiesmentioning

confidence: 71%

“…Therefore, biosolids are generally considered safe for agriculture use (Gerba and Pepper, 2009;Egan, 2013;Brooks et al, 2015;Al-Gheethi et al, 2018;Whitehouse et al, 2022). However, it is shown that some pathogenic bacteria could still survive these processes and end up in biosolid-amended soils (Alam et al, 2007;Viau and Peccia, 2009a;Duceppe et al, 2019), some of which, including Listeria monocytogenes (Al-Ghazali and Al- Azawi, 1990) and Staphylococcus aureus (Lewis and Gattie, 2002) contaminate crops and environment. Moreover, many bacteria within the sewage matrix could carry ARGs, and some of these ARGs get concentrated during treatment processes resulting in a higher concentration of ARGs in biosolids (Munir et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic resistomes and microbial communities in biosolid fertilizers collected from two Canadian wastewater treatment plants in a 10-years interval-potential risks to food chains?

Kang

Naushad

Hartke

et al. 2022

Front. Food. Sci. Technol.

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Dissemination of microorganisms with antimicrobial resistance genes (ARGs) through the food chain has been recognized as a growing public health concern worldwide. Biosolids, a product of wastewater treatment process, have been used as fertilizers in agriculture globally and have also been considered as a potential source of pathogens and ARGs for horizontal transfer across various environments. This study characterized antibiotic resistomes and microbiota in 24 biosolids samples collected from two Canadian waste water treatment plants in different cities in 2009 and 2019. The ARGs were detected using a qPCR array kit, and microbiota was analyzed using 16S ribosomal RNA gene amplicon sequencing. Furthermore, correlation analysis of ARG abundance and bacterial genera abundance was explored to predict potential hosts of ARGs. Seventy-one of 84 ARGs were detected in at least one or more samples with 12 ARGs being detected in all samples. Antibiotic resistomes did not show a statistically significant distinction between different collection years, sites, or year and site combined in principle coordinate analysis. The microbiota communities were significantly different between samples collected in different years, sites, or year and site combined. In total 34 phyla were detected with 13 genera among the top three phyla were typically related to the human gut microbiota and seven of them showing strong correlation with ARGs related to aminoglycoside and beta-lactam resistance. This study provides valuable baseline information and consistent trend on ARGs and bacterial communities in biosolid fertilizers in Canada, indicating that the biosolid fertilizer could potentially be a source of ARGs in the agricultural soils and may leading to potential contamination of plant-based food chains.

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Section: Study Sites and Sample Collectionsupporting

confidence: 79%

Section: Co-occurrence Patterns Between Args and Bacterial Communitiesmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibiotic resistomes and microbial communities in biosolid fertilizers collected from two Canadian wastewater treatment plants in a 10-years interval-potential risks to food chains?

Kang

Naushad

Hartke

et al. 2022

Front. Food. Sci. Technol.

Self Cite

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show abstract

“…Citrobacter spp. with an average relative abundance of 1.45% maintained a low growth during storage, which have been considered as pathogen bacteria and associated with many diseases including septicemia in animals even human being (Duceppe et al, 2019). Interestingly, Lactobacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Unraveling characterizations of bacterial community and spoilage profiles shift in chilled pork during refrigerated storage

Wang

Deng²,

Sun

et al. 2022

Food Sci. Technol

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Changes in bacterial community composition and bacterial counts of chilled pork during storage at 4 °C were unraveled by culture-dependent method and culture-independent method. Physical and chemical analyses including drip loss, total volatile basic nitrogen (TVB-N), pH, and surface color were also performed to estimate its quality and shelf life. A total of 37 phyla, 575 genera and 843 species were identified in all samples by high-throughput sequencing technology during 10 days' storage. At the phylum level, Proteobacteria and Firmicutes were the dominant phylum. At the genus level, Pseudomonas spp., Acinetobacter spp., Pantoea spp., Brochothrix spp. and Raoultella spp. were the dominant genera with their average relative abundance above 5%. In addition, 12 species with average relative abundance more than 1% were found. These dominant bacteria were main pathogenic or spoilage bacteria, and seriously affected the quality of chilled meat. Based on the results of total viable counts (TVC), TVB-N level and sensory evaluation, the shelf life of chilled pork stored at 4 °C was no more than 3 days. Through the analyses of the TVC and the microbial community structure during the spoilage of chilled pork, the main microorganisms causing spoilage were revealed, which will guide significance for further control microbial quality of chilled pork.

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“…Wastewater treatment plants (WWTPs) have been regarded as the major source of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) 11,12. Previous studies have reported the isolation of several C. freundii strains from WWTPs 13,14. In our investigation of the ARB and ARGs in WWTPs, we obtained a multidrug-resistant strain of C. freundii .…”

Section: Introductionmentioning

confidence: 99%

<p>Genetic characterization of a novel sequence type of multidrug-resistant <em>Citrobacter freundii</em> strain recovered from wastewater treatment plant</p>

Jiang

Cui

Liu

et al. 2019

IDR

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A multidrug-resistant Citrobacter freundii strain R17 was isolated from a wastewater treatment plant in China. Whole-genome sequencing of strain R17 revealed a new sequence type (ST412) chromosome (length 5,124,258 bp) and an Inc FII (Yp) group plasmid pCFR17_1 (length 206,820 bp). A total of 13 antibiotic-resistance genes (ARGs) that confer resistance to eight different antibiotic groups were encoded by strain R17 and 12 of them were carried by plasmid pCFR17_1. These data and analysis suggest that the environment-derived C. freundii strains may serve as potential sources of ARGs and highlight the need of further surveillance of this bacteria in the future.

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Draft Genome Sequences of Eight Canadian Citrobacter braakii and Citrobacter freundii Strains

Cited by 10 publications

References 11 publications

Antibiotic resistomes and microbial communities in biosolid fertilizers collected from two Canadian wastewater treatment plants in a 10-years interval-potential risks to food chains?

Antibiotic resistomes and microbial communities in biosolid fertilizers collected from two Canadian wastewater treatment plants in a 10-years interval-potential risks to food chains?

Unraveling characterizations of bacterial community and spoilage profiles shift in chilled pork during refrigerated storage

<p>Genetic characterization of a novel sequence type of multidrug-resistant <em>Citrobacter freundii</em> strain recovered from wastewater treatment plant</p>

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