&lt;p&gt;Genetic characterization of a novel sequence type of multidrug-resistant &lt;em&gt;Citrobacter freundii&lt;/em&gt; strain recovered from wastewater treatment plant&lt;/p&gt;

Jiang, Xin; Cui, Xinjie; Liu, Wenhong; Xu, Hao; Zheng, Beiwen

doi:10.2147/idr.s213525

Cited by 13 publications

(8 citation statements)

References 25 publications

(19 reference statements)

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“…This result further supported the previous hypothesis that bla CMY genes were ubiquitous in CFC isolates. 19 S1-PFGE and Southern blot demonstrated that DY2019 encoded two plasmids (Figure 1), and bla NDM-1 was located on a ~348 kb novel IncHI2 plasmid (Figure 2). Interestingly, the principal NDM variant found in CFC was bla NDM−1 , which is usually located on the self-transferable IncX3 plasmid.…”

Section: Resultsmentioning

confidence: 99%

“…18 Bacterial identification was performed by both VITEK2 compact system (BioMerieux, France) and Microflex MALDI-TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) as described previously. 6,19 Using ANI analysis, species were determined if the genome in question had >95% ANI value with the type genome using pyani with default settings (https://github.com/widdowquinn/pyani).…”

Section: Methodsmentioning

confidence: 99%

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Co-Production of NDM-1 and OXA-10 β-Lactamase in Citrobacter braakii Strain Causing Urinary Tract Infection

Han

Zhao

Yang

et al. 2022

IDR

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In this study, we describe, for the first time, the co-existence of bla NDM-1 and bla OXA-10 in a carbapenem-resistant Citrobacter braakii strain DY2019 isolated from a patient with urinary tract infection in China. We aimed to investigate the genomic context of two β-lactamase-producing plasmids and characterize the transmission mechanism of the carbapenemase-encoding gene. Whole-genome sequencing of strain DY2019 was performed with Nanopore and Illumina platforms, which revealed a chromosome sequence with the length of 4,830,928 bp, an IncC group plasmid pDY2019-OXA (size of 178,134 bp), and a novel IncHI2 group plasmid pDY2019-NDM (length 348,495 bp). A total of 16 antimicrobial resistance genes (ARGs) that confer resistance to nine different antibiotic groups were identified in strain DY2019, and 11 of them were carried by plasmid pDY2019-OXA. These data and analyses suggest that the carbapenem-resistant C. braakii strains may serve as potential reservoir of carbapenemase and highlight the need for further close surveillance of this species in clinical settings.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Co-Production of NDM-1 and OXA-10 β-Lactamase in Citrobacter braakii Strain Causing Urinary Tract Infection

Han

Zhao

Yang

et al. 2022

IDR

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“…Therefore, Citrobacter spp. has been speculated as a potential source for carrying and spreading of antibiotic resistant genes ( Jiang et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Emergence of plasmid-mediated colistin resistance mcr-3.5 gene in Citrobacter amalonaticus and Citrobacter sedlakii isolated from healthy individual in Thailand

Phuadraksa

Wichit

Songtawee

et al. 2023

Front. Cell. Infect. Microbiol.

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Citrobacter spp. are Gram-negative bacteria commonly found in environments and intestinal tracts of humans and animals. They are generally susceptible to third-generation cephalosporins, carbapenems and colistin. However, several antibiotic resistant genes have been increasingly reported in Citrobacter spp., which leads to the postulation that Citrobacter spp. could potentially be a reservoir for spreading of antimicrobial resistant genes. In this study, we characterized two colistin-resistant Citrobacter spp. isolated from the feces of a healthy individual in Thailand. Based on MALDI-TOF and ribosomal multilocus sequence typing, both strains were identified as Citrobacter sedlakii and Citrobacter amalonaticus. Genomic analysis and S1-nuclease pulsed field gel electrophoresis/DNA hybridization revealed that Citrobacter sedlakii and Citrobacter amalonaticus harbored mcr-3.5 gene on pSY_CS01 and pSY_CA01 plasmids, respectively. Both plasmids belonged to IncFII(pCoo) replicon type, contained the same genetic context (Tn3-IS1-ΔTnAs2-mcr-3.5-dgkA-IS91) and exhibited high transferring frequencies ranging from 1.03×10-4 - 4.6×10-4 CFU/recipient cell Escherichia coli J53. Colistin-MICs of transconjugants increased ≥ 16-fold suggesting that mcr-3.5 on these plasmids can be expressed in other species. However, beside mcr, other major antimicrobial resistant determinants in multidrug resistant Enterobacterales were not found in these two isolates. These findings indicate that mcr gene continued to evolve in the absence of antibiotics selective pressure. Our results also support the hypothesis that Citrobacter could be a reservoir for spreading of antimicrobial resistant genes. To the best of our knowledge, this is the first report that discovered human-derived Citrobacter spp. that harbored mcr but no other major antimicrobial resistant determinants. Also, this is the first report that described the presence of mcr gene in C. sedlakii and mcr-3 in C. amalonaticus.

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“…are common Gram-negative bacilli and widely found in water, food, soil, and intestines of animals and humans [ 21 ]. AMR encoding genes are frequently reported in Citrobacter freundii , and it became a reservoir of antibiotic resistance genes in recent years [ 22 , 23 ]. In addition, MDR C. freundii has been reported in numerous hosts including but are not limited to humans [ 24 – 26 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic characterization of multidrug-resistant Citrobacter spp. strains in Fennec fox imported to China

et al. 2021

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Background To investigate the antimicrobial profiles and genomic characteristics of MDR-Citrobacter spp. strains isolated from Fennec fox imported from Sudan to China. Methods Four Citrobacter spp. strains were isolated from stool samples. Individual fresh stool samples were collected and subsequently diluted in phosphate buffered saline as described previously. The diluted fecal samples were plated on MacConkey agar supplemented with 1 mg/l cefotaxime and incubated for 20 h at 37 °C. Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI–TOF–MS) was used for identification. Antimicrobial susceptibility testing was performed using the broth microdilution method. Whole-genome sequencing was performed on an Illumina Novaseq-6000 platform. Acquired antimicrobial resistance genes and plasmid replicons were detected using ResFinder 4.1 and PlasmidFinder 1.3, respectively. Comparative genomic analysis of 277 Citrobacter genomes was also performed. Results Isolate FF141 was identified as Citrobacter cronae while isolate FF371, isolate FF414, and isolate FF423 were identified as Citrobacter braakii. Of these, three C. braakii isolates were further confirmed to be extended-spectrum β-lactamases (ESBL)-producer. All isolates are all multidrug resistance (MDR) with resistance to multiple antimicrobials. Plasmid of pKPC-CAV1321 belong to incompatibility (Inc) group. Comparative genomics analysis of Citrobacter isolates generated a large core-genome. Genetic diversity was observed in our bacterial collection, which clustered into five main clades. Human, environmental and animal Citrobacter isolates were distributed into five clusters. Conclusions To our knowledge, this is the first investigation of MDR-Citrobacter from Fennec Fox. Our phenotypic and genomic data further underscore the threat of increased ESBL prevalence in wildlife and emphasize that increased effort should be committed to monitoring the potentially rapid dissemination of ESBL-producers with one health perspective.

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<p>Genetic characterization of a novel sequence type of multidrug-resistant <em>Citrobacter freundii</em> strain recovered from wastewater treatment plant</p>

Cited by 13 publications

References 25 publications

Co-Production of NDM-1 and OXA-10 β-Lactamase in Citrobacter braakii Strain Causing Urinary Tract Infection

Co-Production of NDM-1 and OXA-10 β-Lactamase in Citrobacter braakii Strain Causing Urinary Tract Infection

Emergence of plasmid-mediated colistin resistance mcr-3.5 gene in Citrobacter amalonaticus and Citrobacter sedlakii isolated from healthy individual in Thailand

Comparative genomic characterization of multidrug-resistant Citrobacter spp. strains in Fennec fox imported to China

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