Antibiotic resistomes and microbial communities in biosolid fertilizers collected from two Canadian wastewater treatment plants in a 10-years interval-potential risks to food chains?

Abstract

Dissemination of microorganisms with antimicrobial resistance genes (ARGs) through the food chain has been recognized as a growing public health concern worldwide. Biosolids, a product of wastewater treatment process, have been used as fertilizers in agriculture globally and have also been considered as a potential source of pathogens and ARGs for horizontal transfer across various environments. This study characterized antibiotic resistomes and microbiota in 24 biosolids samples collected from two Canadian waste water treatment plants in different cities in 2009 and 2019. The ARGs were detected using a qPCR array kit, and microbiota was analyzed using 16S ribosomal RNA gene amplicon sequencing. Furthermore, correlation analysis of ARG abundance and bacterial genera abundance was explored to predict potential hosts of ARGs. Seventy-one of 84 ARGs were detected in at least one or more samples with 12 ARGs being detected in all samples. Antibiotic resistomes did not show a statistically significant distinction between different collection years, sites, or year and site combined in principle coordinate analysis. The microbiota communities were significantly different between samples collected in different years, sites, or year and site combined. In total 34 phyla were detected with 13 genera among the top three phyla were typically related to the human gut microbiota and seven of them showing strong correlation with ARGs related to aminoglycoside and beta-lactam resistance. This study provides valuable baseline information and consistent trend on ARGs and bacterial communities in biosolid fertilizers in Canada, indicating that the biosolid fertilizer could potentially be a source of ARGs in the agricultural soils and may leading to potential contamination of plant-based food chains.

Characterizing the Opportunity Space for Sustainable Hydrothermal Valorization of Wet Organic Wastes

“…For example, food wastethe single largest component of municipal solid waste sent to landfillsrepresents more than 15% of total U.S. human-caused methane emissions, and nutrients in these wastes can cause additional environmental consequences (e.g., via leachate and runoff). Other common resource recovery methods, such as incineration and anaerobic digestion with land application, can also lead to unwanted environmental consequences including fugitive NO x emissions during incineration or the release of antibiotic resistant bacteria, , heavy metals, or emerging contaminants like per- and polyfluorinated substances (PFAS) from the land application of biosolids. Further, these management methods are costly (40 to 880 $·tonne –1 , ) and often underutilize the resource potential embedded in organic wastes.…”