Downregulation of the long non-coding RNA XLOC_010588 inhibits the invasion and migration of colorectal cancer

Li, Yue; Zhao, Lin; Zhang, Yining; Lin, Guozhen; Zhang, Huijing; Zhou, Huan; Gao, Tong; Miao, Peng; Sun, Mingjun

doi:10.3892/or.2018.6260

Cited by 11 publications

(11 citation statements)

References 30 publications

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“…In addition, dysregulated lncRNAs were documented to act as tumor promoters or suppressors in colon cancer. Li et al found that downregulated lncRNA XLOC-010588 inhibits colon cancer cell invasion and migration [28]. Consistent with our study, a previous study has indicated that shRNA against CD44 functions…”

Section: Discussionsupporting

confidence: 93%

LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer

Liu

et al. 2020

Med Sci Monit

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Departmental sources Background: We explored the regulatory effects of long noncoding RNA (lncRNA) LOC101060264 silencing mediated by shRNA on invasion and metastasis of human colon cancer. Material/Methods: Initially, 2 shRNA plasmids for LOC101060264 silencing-shRNA1 and shRNA2-were introduced into LoVo cells. Following transfection, the expressions of LOC101060264, E-cadherin, and vimentin were determined. Next, to explore the regulatory effects of LOC101060264 silencing on cell growth, cell cycle, invasion, and migration abilities of LoVo cells, we performed MTT, flow cytometry, Transwell assay, and scratch assay, respectively. Furthermore, in nude mice with xenografted tumors, the tumor volume and weight were measured, and the expressions of PCNA, E-cadherin, vimentin, and MMP-9 in tumor tissues were determined by immunohistochemistry. Results: The level of E-cadherin increased and the level of vimentin decreased after LOC101060264 silencing mediated by shRNA1 and shRNA2 in LoVo cells. Silencing LOC101060264 repressed the migration, invasion, and proliferation of LoVo cells in vitro and inhibited tumor growth in nude mice in vivo. We also studied the expression of these proteins and found reduced expression of PCNA, vimentin, and MMP-9 protein, and found enhanced expression of E-cadherin protein. Moreover, the inhibitory effect of shRNA2 on the above cell behaviors was stronger than that of shRNA1. Conclusions: In summary, LOC101060264 silencing decreased LoVo cell invasiveness via suppressing ETM and attenuated tumor metastasis, which provides a novel therapeutic target for patients with colon cancer.

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Section: Discussionsupporting

confidence: 93%

LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer

Liu

et al. 2020

Med Sci Monit

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“…TUSC8 is an lncRNA that participates in many cancer progression. Li et al () have demonstrated that TUSC8 is closely associated with metastasis and poor prognosis of colorectal cancer. Peng et al () reported that TUSC8 might be a potential diagnostic biomarker for CC, but its role was not clear.…”

Section: Discussionmentioning

confidence: 99%

LncRNA TUSC8 inhibits the invasion and migration of cervical cancer cells via miR‐641/PTEN axis

Zhu

Liu

Zhang

et al. 2019

Cell Biology International

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Cervical cancer (CC) is the fourth leading cause of cancer‐related death in women worldwide. There is an urgent need to find novel targets for the treatment of CC. Recently, microRNA have emerged as critical factors in tumorigenesis. In this study, we aimed to investigate the mechanism of miR‐641 on the migration and invasion of CC cells. In silico analysis revealed putative interaction between miR‐641 and phosphatase and tensin homolog (PTEN) RNA/lncRNA tumor suppressor candidate 8 (TUSC8). Hence we evaluated the expression of TUSC8, miR‐641, and PTEN. We found that the expressions of TUSC8 and PTEN were decreased in CC tissues, whereas miR‐641 expression was increased. Inhibition of miR‐641 suppressed the migration and invasion of Hela cells. In addition, TUSC8 and PTEN were upstream and downstream target molecule of miR‐641, respectively. Overexpression of TUSC8 promoted PTEN expression, and suppressed the invasion and migration of Hela cells, whereas miR‐641 mimic treatment changed the effects. These results demonstrated that overexpression of TUSC8 could inhibit the invasion and migration of CC cells by upregulating PTEN via miR‐641.

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“…However, XLOC_010588 might function as an oncogene in colorectal cancer. Down-regulation of XLOC_010588 inhibited the invasion and migration of colorectal cancer cells through regulating genes associated with EMT [14]. Furthermore, TUSC8 (LINC01071) showed consistent down-regulation in gastric cancer (GC) compared with adjacent non-tumor tissues and was significantly correlated with the age and gender of the GC patients, respectively [15,16].…”

Section: Agingmentioning

confidence: 99%

Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis

Zhao

Zhou

Zhao

et al. 2020

Aging

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The lncRNA tumor suppressor candidate 8 (TUSC8) plays a critical role in the development of several cancers. However, the biological functions and underlying molecular mechanisms of TUSC8 with respect to breast cancer remain largely unclear. Here, we found that TUSC8 was significantly down-regulated in breast cancer tissues and its high expression predicted better prognosis of breast cancer patients. Functionally, knock-down of TUSC8 drastically promoted the proliferation, migration and invasion of breast cancer cells in vitro and facilitated tumorigenicity and metastasis in vivo. Mechanistically, the results of luciferase reporter, RIP and RNA pulldown assays proved that TUSC8 functioned as molecular sponge for miR-190b-5p. Furthermore, we showed that TUSC8 served as a competing endogenous RNA (ceRNA) of myosin regulatory light chain interacting protein (MYLIP) through competitively binding with miR-190b-5p and suppressed breast cancer metastasis through regulating the expression of epithelial-mesenchymal transition (EMT) related markers. Clinically, the receiver operating characteristic curve (ROC) analyses revealed that the combination usage of TUSC8 and MYLIP might become novel promising diagnostic biomarkers for breast cancer. Taken together, these results suggested that TUSC8 inhibited breast cancer growth and metastasis via miR-190b-5p/MYLIP axis, providing us new insights into developing potential therapeutic targets for breast cancer patients.

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Downregulation of the long non-coding RNA XLOC_010588 inhibits the invasion and migration of colorectal cancer

Cited by 11 publications

References 30 publications

LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer

LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer

LncRNA TUSC8 inhibits the invasion and migration of cervical cancer cells via miR‐641/PTEN axis

Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis

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