LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer

Yu, Weihua; Liu, Lan; Li, Shuai; Zhu, Kongxi

doi:10.12659/msm.920270

Cited by 3 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Earlier studies have already revealed that the overexpression of LINK-A lncRNA boosted cell growth, inhibited apoptosis, and upregulated survivin expression, while knockdown of LINK-A lncRNA had the reverse effect in triple-negative breast cancer 20 . In addition, other scholars have previously reported that the silencing of LOC101060264 suppressed tumor proliferation in nude mice in vivo and suppressed the migration, invasion and proliferation of colon cancer cells in vitro 21 . Furthermore, lncRNAs are also quite prospering for clinical applications in oncology.…”

Section: Discussionmentioning

confidence: 92%

LncRNA EPB41L4A-AS1 Regulates Cell Proliferation, Apoptosis and Metastasis in Breast Cancer

Yang

2021

Preprint

View full text Add to dashboard Cite

Background: Breast cancer is a leading global public health problem. In our previous study, we identified that lncRNA EPB41L4A antisense RNA 1 (EPB41L4A-AS1) was significantly downregulated in breast cancer. However, the functional role of EPB41L4A-AS1 in breast cancer has not been clarified. Here, we further confirmed the expression and biological function of EPB41L4A-AS1 in breast cancer. Methods: To demonstrate the role of EPB41L4A-AS1 in breast cancer, we transfected breast cancer lines with pcDNA3.1-EPB41L4A-AS1 expression vector to induce ectopic overexpression of EPB41L4A-AS1. Then, to explore the role of EPB41L4A-AS1 overexpression in breast cancer cell growth, cell cycle, apoptosis, invasion and migration capacity, we performed CCK-8 assay, colony formation assay, flow cytometry analysis, wound recovery and transwell assay, respectively. We also constructed a co-expression network to explore the potential effect mechanism of EPB41L4A-AS1. Results: Our research showed EPB41L4A-AS1 expression was significantly lower in tumor tissues than in adjacent non-cancerous tissues. overexpression of EPB41L4A-AS1 significantly reduced the proliferation of breast cancer cells. Flow cytometric analysis showed that forced expression of EPB41L4A-AS1 significantly increased the apoptosis rate of breast cancer cells. In addition, we found that upregulated EPB41L4A-AS1 significantly inhibited the migration and invasive ability of breast cancer. Functional analysis of co-expressed mRNAs suggested that EPB41L4A-AS1 may be involved in ribosomal, cell cycle, spliceosomal and p53 signaling pathways. Conclusions: Our findings suggest that EPB41L4A-AS1 is a tumor suppressor gene in breast cancer.

show abstract

Section: Discussionmentioning

confidence: 92%

LncRNA EPB41L4A-AS1 Regulates Cell Proliferation, Apoptosis and Metastasis in Breast Cancer

Yang

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…MMP-9 or MMP-2 has been shown to play an important role in regulating metastasis [17]. We studied whether MMP-2 and MMP-9 were involved in PM-induced cell migration and invasion.…”

Section: Surfactin Inhibits Pm-induced Mmp-2 and Mmp-9 Expression Andmentioning

confidence: 99%

Surfactin from Bacillus subtilis attenuates ambient air particulate matter-promoted human oral cancer cells metastatic potential

Lee

et al. 2020

J. Cancer

View full text Add to dashboard Cite

Recently, many studies have indicated that ambient air particulate matter (PM) can increase the risk of oral cancer. The most common malignant tumor in the oral cavity is oral squamous cell carcinoma (OSCC). Usually, cancer cell migration/invasion is the most important cause of cancer mortality. Matrix metalloproteinase-2 (MMP-2) and MMP-9 have been shown to play important roles in regulating metastasis and the tumor microenvironment. Here, we studied the anti-cancer effects of surfactin, a cyclic lipopeptide generated by Bacillus subtilis, on cancer cell migration and invasion. Surfactin suppressed PM-promoted cell migration and invasion and colony formation of SCC4 and SCC25 human oral squamous cell carcinoma cell lines. We observed that PM induced MMP-2 and MMP-9 expression, which was inhibited by surfactin. Transfection with p65, p50, c-Jun, c-Fos, p85, p110, Akt, mammalian target of rapamycin (mTOR), or interleukin-6 (IL-6) siRNA markedly inhibited PM-induced MMP-2 and MMP-9 expression. Moreover, surfactin could reduce Akt, mTOR, p65, and c-Jun activation and IL-6 secretion induced by PM. Finally, we proved that transfection with Akt, p65, or c-Jun siRNA significantly inhibited PM-induced IL-6 release. Taken together, these results suggest that surfactin functions as a suppressor of PM-induced MMP2/9-dependent oral cancer cell migration and invasion by inhibiting the activation of phosphoinositide 3-kinase (PI3K)/Akt/mTOR and PI3K/Akt/nuclear factor-κB (NF-κB) and activator protein-1 (AP-1)/IL-6 signaling pathways.

show abstract

Identifying family effort factors in preventing Covid-19 transmission in rural areas

Addiarto¹,

Narsih²,

Taufiq

2023

The 4TH INTERNATIONAL CONFERENCE ON LIFE SCIENCE AND TECHNOLOGY (ICoLiST)

View full text Add to dashboard Cite

LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer

Cited by 3 publications

References 29 publications

LncRNA EPB41L4A-AS1 Regulates Cell Proliferation, Apoptosis and Metastasis in Breast Cancer

LncRNA EPB41L4A-AS1 Regulates Cell Proliferation, Apoptosis and Metastasis in Breast Cancer

Surfactin from Bacillus subtilis attenuates ambient air particulate matter-promoted human oral cancer cells metastatic potential

Identifying family effort factors in preventing Covid-19 transmission in rural areas

Contact Info

Product

Resources

About