Downregulation of MACC1 inhibits invasion, migration and proliferation, attenuates cisplatin resistance and induces apoptosis in tongue squamous cell carcinoma

Abstract

The aim of the present study was to investigate the expression and function of metastasis-associated in colon cancer 1 (MACC1) in tongue squamous cell carcinoma (TSCC) and its relationship with the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) (CD147). Levels of MACC1 and EMMPRIN expression were analyzed in 65 paraffin‑embedded tissue specimens of TSCC and in the adjacent non-cancerous tissues using immunohistochemistry (IHC). MACC1 expression was highly associated with lymphatic metastasis and EMMPRIN expression. Overexpression of MACC1 was significantly correlated with poor overall patient survival. A small interfering RNA (siRNA) was delivered into TSCCA cells to downregulate MACC1 expression. The CCK-8 assay showed that TSCCA cell proliferation was markedly reduced and that cisplatin resistance was attenuated. The suppression of MACC1 promoted the apoptosis of the TSCCA cell line. A Transwell experiment demonstrated that the migration and invasion abilities of the TSCCA cells were extremely downregulated. An ELISA experiment and western blotting revealed that the secretion of urokinase-type plasminogen activator system (uPA) in the supernatant of the culture medium and uPA expression were significantly reduced. Experiments revealed that the secretion of metalloproteinase 2 (MMP2) in the supernatant of the culture medium and MMP2 expression were not affected. MACC1 may serve as a novel biomarker and therapeutic target for TSCC.

The role of metastasis‐associated in colon cancer 1 (MACC1) in endometrial carcinoma tumorigenesis and progression

“…MACC1 is known to induce proliferation, migration, and invasion in cell culture . We confirmed this observation by showing that the downregulation of MACC1 through siRNA transfection induced G1 phrase arrest, suppressed cell proliferation, migration, and invasion ability.…”

Investigation of MACC1 Gene Expression in Head and Neck Cancer and Cancer Stem Cells

“…According to literature, MACC1 overexpression has been found in head and neck cancers, specially in tongue squamous cell carcinoma [13]. Results of our experiments showed an induced MACC1 expression of head and neck CSC; even in conmparison with non-CSC cancer cells it showed a two-fold increase.…”

“…In conformity with the result we examined if this gene could be a competitor gene for chemotherapy. According to literature, the MACC1 gene shows a clear expression in head and neck cancer cells [1]. Here we examined MACC1 expression in CSC and investigated it as a possible biomarker.…”

MicroRNA expression and its implications for diagnosis and therapy of tongue squamous cell carcinoma

“…Lymph node and distant metastasis are the most reliable adverse prognostic factors in TSCC patients . Despite recent advances in its diagnostic techniques and therapeutic managements, the number of deaths associated with TSCC increased by over 10% during the past 5 years . It is, therefore, of paramount importance to understand the molecular pathways of carcinogenesis or progression to develop novel therapeutic strategies of TSCC.…”