The role of metastasis‐associated in colon cancer 1 (MACC1) in endometrial carcinoma tumorigenesis and progression

Chen, Shuo; Zong, Zhi‐Hong; Wu, Dandan; Sun, Kaixuan; Liu, Bo-Liang; Zhao, Yang

doi:10.1002/mc.22599

Cited by 22 publications

(17 citation statements)

References 44 publications

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“…Activated HSCs displayed increased expression levels of MMP-2 and MMP-9, which are involved in ECM degradation and vascularization, allowing cancer cells to migrate out of the primary tumor and to form metastases (19). The present results are consistent with Chen et al (20), that reported that MACC1 downregulation inhibited α-SMA, MMP-2, and MMP-9 mRNA or protein expression levels following transfection of endometrial carcinoma cells with MACC1 small interfering RNA. In addition, the present study used HSCs as a chemoattractant source for gastric cancer cells in Transwell assays.…”

Section: Macc1 Was First Reported By Stein Et Alsupporting

confidence: 93%

MACC1 and HGF are associated with survival in patients with gastric cancer

Dong

Wang

et al. 2017

Oncol Lett

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Abstract. Metastasis-associsated in colon cancer 1 (MACC1), a newly identified oncogene, promotes tumor cell proliferation and invasion. In the present study, the expression of MACC1, hepatocyte growth factor (HGF) and its receptor, MET proto-oncogene (c-Met), was investigated in human gastric cancer tissues and adjacent normal tissues by immunohistochemistry. The association between the expression levels of the proteins and the clinicopathological parameters of the tumors were statistically analyzed. Furthermore, lentiviral particles expressing MACC1 were used to infect the hepatic satellite cell (HSC) line LX2. The expression of α-smooth muscle actin (SMA), HGF, matrix metallopeptidase (MMP)-2 and MMP-9 in human HSCs was examined by western blotting and reverse transcription-quantitative polymerase chain reaction. Transwell assays were used to measure the effect of MACC1-infected or non-infected HSCs on the migration and invasion abilities of MKN45 and MKN74 gastric carcinoma cells in vitro. The results demonstrated that positive protein expression of MACC1, HGF and c-Met was significantly higher in human gastric cancer tissues compared with adjacent normal tissues. Positive expression of MACC1 and c-Met in gastric cancer tissues had no correlation with the sex, age, tumor location and peritoneal metastasis of patients, but was significantly correlated with tumor size, depth of tumor invasion, lymph node metastasis, TNM stage, histological differentiation, and overall (5 years) and disease-free survival (5 years). Positive expression of each MACC1, HGF and c-Met protein was demonstrated to be positively correlated with each other in human gastric cancer tissues. Western blotting results confirmed that MACC1 protein was overexpressed in MACC1-overexpressing lentivirus-infected HSCs. Overexpression of MACC1 significantly increased HGF, MMP-2, MMP-9 and α-SMA expression levels in HSCs. Results from the Transwell assays indicated an increase in the number of MKN45 or MKN74 cells migrating towards MACC1-overexpressing HSCs, compared with control HSCs. These findings suggested that MACC1 may regulate the expression of HGF, MMP-2 and MMP-9 in HSCs, and may thus promote migration and invasion of gastric carcinoma cells. MACC1, HGF and c-Met might cooperatively participate in the malignant progression of gastric cancer. In conclusion, MACC1 might serve as a useful molecular target for the diagnosis, treatment and prognosis of gastric cancer.

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Section: Macc1 Was First Reported By Stein Et Alsupporting

confidence: 93%

MACC1 and HGF are associated with survival in patients with gastric cancer

Dong

Wang

et al. 2017

Oncol Lett

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“…MiR-23b-3p overexpression inhibits the proliferative capability of breast cancer cells [23,24], ovarian cancer cells [25], hepatocarcinoma cells [18], colorectal cancer cells [21], and endometrial cancer cells [26]. However, a study conducted by Wang et al yielded inconsistent results [27].…”

Section: Discussionmentioning

confidence: 76%

Biological Effect and Mechanism of the miR-23b-3p/ANXA2 Axis in Pancreatic Ductal Adenocarcinoma

Wei

Dang

Feng

et al. 2018

Cell Physiol Biochem

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Background/Aims: Accumulating evidence strongly suggests that microRNAs (miRNAs) modulate the expression of known tumor suppressor genes and oncogenes. In the present study, we found that the proliferation and invasion ability of pancreatic ductal adenocarcinoma (PDAC) cells were significantly suppressed by the overexpression of miR-23b-3p. In addition, there are miR-23b-3p binding sites in annexin A2 (ANXA2). Here, we investigated whether miR-23b-3p had an impact on the progression and metastasis of PDAC by targeting ANXA2. Methods: Cell proliferation, migration, and invasion, and cell cycle assays were performed to explore the effect of miR-23b-3p on various malignant phenotypes of pancreatic cancer cells. The size of tumors was observed following miR-23b-3p overexpression in an in vivo chick chorioallantoic membrane assay. Dual-luciferase reporter, quantitative real-time PCR, western blot, and immunohistochemical analyses were used to validate the relationship between miR-23b-3p and ANXA2 in vitro. Results: We observed that miR-23b-3p could bind specifically to the 3′ untranslated region of ANXA2 and inhibit its expression. MiR-23b-3p overexpression downregulated the expression of ANXA2 mRNA in PDAC cells and limited the size of tumors or even prevented tumor formation. In addition, there was a negative correlation between miR-23b-3p expression and ANXA2 protein expression in clinical specimens. Conclusion: MiR-23b-3p inhibits the development and progression of PDAC by regulating ANXA2 directly.

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“…In our studies, MMP2 levels were found to be higher in non-endometrioid carcinomas than in endometrioid adenocarcinomas. Karahan et al23 and Chen et al12 demonstrated that MMP2 expression was much higher in patients with higher clinical stage of the disease. Higher expression of MMP2 in higher tumor stage patients was also demonstrated in immunohistochemical studies by Graesslin et al24 and Li et al25 We observed that the serum MMP2 levels were markedly higher in patients with higher clinical stage of the disease (FIGO III and IV).…”

Section: Discussionmentioning

confidence: 99%

“…The most recent studies by Chen et al12 showed that MACC1 plays an important role in endometrial cancer, as its high expression confirmed in vivo in endometrial cancer cell lines was correlated with high expression of MMP2. MACC1 is currently considered a key regulator in the development of numerous cancers.…”

Section: Discussionmentioning

confidence: 99%

Clinical importance of serum HE4 and MMP2 levels in endometrial cancer patients

Cymbaluk‐Płoska¹,

Chudecka‐Głaz²,

Pius-Sadowska

et al. 2017

OTT

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IntroductionEndometrial cancer is the one of the most common cancers of the genital organ. HE4 and MMP2 are both proteins whose serum levels increase in endometrial cancer.AimTo explore the diagnostic potential of the serum levels of HE4 and MMP2 in patients with endometrial cancer and benign endometrial diseases. To assess the relationship between the serum levels of HE4 and MMP2 and the typical prognostic factors in patients with endometrial cancer.Materials and methodsIncluded in the study was a group of 112 patients presenting with bleeding abnormalities at the Pomeranian Medical University in years 2012–2016. Serum HE4 concentrations were measured using the Elecsys Electrochemiluminescence Immunoassay (ECLIA). MMP2 concentrations were quantified in the serum using multiplex immunoassays.ResultsWe observed statistically significant differences in mean serum levels of HE4 and MMP2 between the group of endometrial cancer patients and the group of patients with no changes in the endometrium (P=0.002/0.003). The diagnostic potential of HE4 and MMP2 in differentiation of high (International Federation of Gynecology and Obstetrics [FIGO] III and IV) vs low (FIGO I and II) clinical stage of tumor and prediction of cellular differentiation grade (G1 vs G3) on the basis of the analysis of the area under the curve is, respectively, 0.86 and 0.82 for HE4 and 0.82 and 0.74 for MMP2. The HE4 marker was significantly more specific than MMP2 in every study group and amounted to 93% vs 86% in all patients included in the analysis, 94% vs 84% in pre-menopausal patients and 84% vs 79% in post-menopausal patients.ConclusionHE4 and MMP2 are characterized by high specificity and may be useful as biomarkers in the diagnostics of endometrial cancer. When determined preoperatively, HE4 is correlated with the prognostic factors of endometrial cancer and may be helpful in the planning of individual treatment of endometrial cancer patients.

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The role of metastasis‐associated in colon cancer 1 (MACC1) in endometrial carcinoma tumorigenesis and progression

Cited by 22 publications

References 44 publications

MACC1 and HGF are associated with survival in patients with gastric cancer

MACC1 and HGF are associated with survival in patients with gastric cancer

Biological Effect and Mechanism of the miR-23b-3p/ANXA2 Axis in Pancreatic Ductal Adenocarcinoma

Clinical importance of serum HE4 and MMP2 levels in endometrial cancer patients

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