Investigation of MACC1 Gene Expression in Head and Neck Cancer and Cancer Stem Cells

Evran, Ebru; Şahin, Hilal; Akbaş, Kübra; Cigdem, Sadik; Gündüz, Esra

doi:10.25011/cim.v39i6.27506

Cited by 3 publications

(3 citation statements)

References 12 publications

(20 reference statements)

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“…50 MACC1 is considered the main regulator of tumor progression in TSCC, in which it affects a number of malignant processes. 33,50 MACC1 affected cancer progression through different mechanisms. For example, MACC1 improves the cisplatin resistance partially in lung cancer via the PI3K/AKT pathway, 51 whereas its depletion suppresses lung cancer cell proliferation and causes cell apoptosis through the β-catenin pathway.…”

Section: Discussionmentioning

confidence: 99%

“…Among these candidates, the 3′-UTR of MACC1 contains complementary binding sequences for miR-876-3 ( Figure 4G) and was chosen for further confirmation because of its wellknown oncogenic actions in TSCC progression. 33 Data from the luciferase reporter assay revealed that the upregulation of miR-876-3p strikingly reduced the luciferase activity of wt-MACC1 in SCC-15 and CAL-27 cells, whereas mutation of the binding site in MACC1 3ʹ-UTR counteracted the suppressive action of miR-876-3p on luciferase activity ( Figure 4H). Furthermore, MACC1 was clearly overexpressed in TSCC tissues than in adjacent normal tissues ( Figure 4I).…”

Section: Macc1 Is a Target Of Mir-876-3p In Tscc Cellsmentioning

confidence: 97%

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<p>Long Intergenic Non-Protein Coding RNA 519 Promotes the Biological Activities of Tongue Squamous Cell Carcinoma by Sponging microRNA-876-3p and Consequently Upregulating MACC1</p>

Liu

Zhao

Wang

et al. 2020

OTT

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Long intergenic non-protein coding RNA 519 (LINC00519) promotes the development of lung squamous cell carcinoma. In this study, we detected the expression of LINC00519 in tongue squamous cell carcinoma (TSCC) and examined its clinical significance. Additionally, the regulatory effects of LINC00519 on behaviors of TSCC tumor cells were explored through functional experiments. Finally, mechanistic studies were performed to elucidate the molecular events underlying the tumor-promoting actions of LINC00519 in TSCC. Materials and Methods: The expression of LINC00519 in TSCC tissues and cell lines was determined using quantitative reverse transcription-polymerase chain reaction. Cell counting kit-8 assay, flow cytometric analysis, cell migration and invasion assays and xenograft tumor model analyses were used to detect TSCC cell proliferation, apoptosis, migration and invasion and in vivo tumor growth, respectively. Mechanistic studies were performed using bioinformatics analysis, RNA immunoprecipitation assay, luciferase reporter assay and rescue experiments. Results: LINC00519 was overexpressed in both TSCC tissues and cell lines. A high LINC00519 level was associated with poor overall survival in patients with TSCC. In vitro, LINC00519 played cancer-promoting roles in TSCC progression by facilitating cell proliferation, migration and invasion and restraining cell apoptosis. In vivo, LINC00519 downregulation resulted in decreased TSCC tumor growth. Mechanistically, LINC00519 acted as a competing endogenous RNA for microRNA-876-3p (miR-876-3p), which directly targets metastasis associated with colon cancer-1 (MACC1), in TSCC cells. LINC00519 upregulated the expression of MACC1 in TSCC cells by sequestering miR-876-3p. Rescue experiments further affirmed that miR-876-3p inhibition or MACC1 overexpression mitigated the inhibitory influences of LINC00519 depletion on cell proliferation, migration and invasion and neutralized the promoting actions of LINC00519 knockdown on cell apoptosis in TSCC. Conclusion: LINC00519 aggravated the oncogenicity of TSCC by regulating the miR-876-3p/MACC1 axis. Our findings suggest that the LINC00519/miR-876-3p/MACC1 pathway may be an underlying therapeutic target in TSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Macc1 Is a Target Of Mir-876-3p In Tscc Cellsmentioning

confidence: 97%

<p>Long Intergenic Non-Protein Coding RNA 519 Promotes the Biological Activities of Tongue Squamous Cell Carcinoma by Sponging microRNA-876-3p and Consequently Upregulating MACC1</p>

Liu

Zhao

Wang

et al. 2020

OTT

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“…Expression of MACC -1 gene is twofold higher in CSCs than in head and neck cancer cells and could be a biomarker for head and neck cancer [13].…”

Section: Identification and Characterization Of Cscs Vs Scsmentioning

confidence: 99%

Stem Cells in Head and Neck Cancers Pathogenesis: Are Advanced Glycation End Products (AGEs) Involved?

Nausica¹,

Anida-Maria²,

Şoriţău³

et al. 2018

Biochem Mol biol J

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Regardless of significant advances over the last years, cancer is still an aggressive and life-threatening disease with a negative impact on the patient's quality of life, socioeconomic status and lifestyle. Finding a cure for cancer has become a major challenge for all researchers and medical professionals worldwide and enormous efforts have been made to find more effective prophylactic and therapeutic approaches. Nowadays, cancer treatment can be aggressive and it is not always well targeted. Therefore, more specific therapies should be focused on tumor cells and pathogenic mechanisms implicated in carcinogenesis. In this regard, our paper aims at discussing the interrelation between cancer stem cells (CSCs) and Advanced glycation end products (AGEs) with possible future research perspectives. CSCs are stem cells with tumorigenic properties that are capable to give rise to all the cell types found in a tumor. Development of innovative therapies targeted on CSCs could improve the survival rate and the quality of life for cancer patients. AGEs are a heterogeneous group of compounds formed by the non-enzymatic reaction between the reducing sugars and proteins, lipids or amino acids. Multiple sources of AGEs have been described, as well as AGEs involvement in numerous pathogenic pathways, carcinogenesis being one of them. In order to better understand the pathogenic mechanisms implicated in carcinogenesis and to develop efficient prophylactic and therapeutic approaches, the interrelation between CSCs and AGEs should be taken into consideration.

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MACC1—the first decade of a key metastasis molecule from gene discovery to clinical translation

Radhakrishnan

Walther

Zincke

et al. 2018

Cancer Metastasis Rev

100

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Investigation of MACC1 Gene Expression in Head and Neck Cancer and Cancer Stem Cells

Cited by 3 publications

References 12 publications

<p>Long Intergenic Non-Protein Coding RNA 519 Promotes the Biological Activities of Tongue Squamous Cell Carcinoma by Sponging microRNA-876-3p and Consequently Upregulating MACC1</p>

<p>Long Intergenic Non-Protein Coding RNA 519 Promotes the Biological Activities of Tongue Squamous Cell Carcinoma by Sponging microRNA-876-3p and Consequently Upregulating MACC1</p>

Stem Cells in Head and Neck Cancers Pathogenesis: Are Advanced Glycation End Products (AGEs) Involved?

MACC1—the first decade of a key metastasis molecule from gene discovery to clinical translation

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