Downregulating microRNA-152-3p promotes the viability and osteogenic differentiation of periodontal ligament stem cells via targeting integrin alpha 5

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

“…At the cellular level, the integrins are important mechano-transducers involved in both matrix deposition and organization. In spite of their relevant role as modulators of TGF-β/BMP and Wnt/β-catenin signaling pathways, to date, few miRNAs targeting integrins have been identified in osteogenic differentiation [189]. Further studies identifying the biological interaction between integrins and miRNAs in osteogenesis are needed [189].…”

The role of integrin family in bone metabolism and tumor bone metastasis

“…Circular RNA VGLL3 (circRNA-VGLL3) promoted the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) through circRNA-VGLl3/miR-326-5p/integrin α 5 pathway [ 25 ]. Integrin α 5 was also found to promote the survival and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) as a target gene of miR-152-3p [ 26 ]. Integrins showed versatility in the early stage of bone metabolism by independently regulating migration and differentiation of BMSCs or mediating other pathways.…”

“… Integrins Cell types Potential pathways or binding protein Regulating effects Ref. α v β 3 BMSCs BSP/MMP-2/α v β 3 Promote cell migration [ 18 ] β 1 NCAM/β 1 /cofilin Regulate migration and promote proliferation [ 14 ] α 5 β 1 WISP-1/α 5 β 1 /BMP-2 Enhance cell adhesion, survival and migration [ 20 ] α 2 β 1 Col II/α 2 β 1 /FAK-JNK Increase bone formation and defect healing [ 23 ] α 5 ADSCs circRNA-VGLl3/microRNA-326-5p/α 5 Promote osteogenic differentiation [ 25 ] hPDLSCs microRNA-152-3p/α 5 Promote survival and osteogenic differentiation [ 26 ] α 5 β 1 , α V β 5 osteoblast CCN3/α 5 β 1 , α V β 5 /BMP-4 Promote cell maturation, adhesion and matrix mineralization [ 28 ] α v β 1 α v β 1 /CCN2/FAK/ERK Enhance the adhesion and differentiation of osteoblasts [ 29 ] α V β 3 , α V β 5 CCN1/α V β 3 , α V β 5 / PTH1R Promotes bone anabolism through activation of PTH signaling [ 30 ] α 2 β 1 Col I/α 2 β 1 , α 2 β 1 /ERK Regulate cell differentiation …”

Recent advancements of miRNAs in the treatment of bone diseases and their delivery potential

“…(2022) 5 miR-214 Chr1 Inhibits the osteoblast function by targeting ATF4 protein and promotes the function of osteoclast through phosphatase and tensin homolog Wang et al. (2019) 6 miR-152 Chr17 Stimulates the viability and osteogenic differentiation of periodontal ligament stem cells by targeting integrin alpha Wu and Ma (2020) 7 miR-134 Chr14 Augments the osteosarcoma chemoresistance through miR-134/PTBP1 signaling cascade Zhang et al. (2021) 8 miR-544 Chr14 Deregulates a gene–protein network and contributes to the pathogenesis of osteosarcoma Thayanithy et al.…”