Doubled haploid wheat lines with high molecular weight glutenin alleles derived from microspore cultures

Barakat, M. N.; Al‐Doss, Abdullah A.; Ghazy, Abdelhalim I.; Moustafa, Khaled A.; El-Shafei, A.; Ahmed, E. I.

doi:10.1080/01140671.2017.1368674

Cited by 10 publications

(7 citation statements)

References 27 publications

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“…Due to its homozygosity, DH wheat can be used as a variety, a parent in the creation of new hybrids, and source of genetic mapping studies. Therefore, determining the genetic diversity of DH wheat lines based on phenotypic traits and DNA polymorphisms is significant to determine strategy in breeding programs ( Barakat et al, 2013 ). It is recommended that the parents are chosen for hybridization from clusters that are far apart in order to obtain greater genetic diversity in the segregating generations and more heterosis in F 1 generation.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genetic diversity of doubled haploid bread wheat population and molecular validation for spike characteristics, end-use quality, and biofortification capacity

Kutlu

Çelik

Karaduman

et al. 2023

PeerJ

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Increasing grain quality and nutritional value along with yield in bread wheat is one of the leading breeding goals. Selection of genotypes with desired traits using traditional breeding selection methods is very time-consuming and often not possible due to the interaction of environmental factors. By identifying DNA markers that can be used to identify genotypes with desired alleles, high-quality and bio-fortified bread wheat production can be achieved in a short time and cost-effectively. In the present study, 134 doubled haploid (DH) wheat lines and their four parents were phenotypically evaluated for yield components (spike characteristics), quality parameters, and grain Fe and Zn concentrations in two successive growing seasons. At the same time, ten genic simple sequence repeats (SSR) markers linked to genes related to the traits examined were validated and subsequently used for molecular characterization of trait-specific candidate genotypes. Significant genotypic variations were determined for all studied traits and many genotypes with desired phenotypic values were detected. The evaluation performed with 10 SSR markers revealed significant polymorphism between genotypes. The polymorphic information content (PIC) values of 10 markers ranged from 0.00 to 0.87. Six out of 10 SSRs could be more effective in representing the genotypic differentiation of the DH population as they demonstrated the highest genetic diversity. Both Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering and STRUCTURE analyses divided 138 wheat genotypes into five (K = 5) main groups. These analyzes were indicative of genetic variation due to hybridization and segregation in the DH population and the differentiation of the genotypes from their parents. Single marker regression analysis showed that both Xbarc61 and Xbarc146 had significant relationships with grain Fe and Zn concentrations, while Xbarc61 related to spike characteristics and Xbarc146 related to quality traits, separately. Other than these, Xgwm282 was associated with spike harvest index, SDS sedimentation value and Fe grain concentration, while Gwm445 was associated with spikelet number, grain number per spike and grain Fe concentration. These markers were validated for the studied DH population during the present study and they could be effectively used for marker-assisted selection to improve grain yield, quality, and bio-fortification capacity of bread wheat.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and genetic diversity of doubled haploid bread wheat population and molecular validation for spike characteristics, end-use quality, and biofortification capacity

Kutlu

Çelik

Karaduman

et al. 2023

PeerJ

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“…Controlled light and temperature conditions (greenhouse, phytotron chamber) offer a good chance for growing donor plants during the whole year (Ghaemi et al, 1995;Torp et al, 2001;Pauk et al, 2003;Tuvesson et al, 2000Tuvesson et al, , 2003Soriano et al, 2007Soriano et al, , 2008Broughton, 2008Broughton, , 2011Redha and Suleman, 2011;Brew-Appiah et al, 2013;Sanchez-Diaz et al, 2013;Castillo et al, 2015;Rubtsova et al, 2013;Nielsen et al, 2015;Seifert et al, 2016;Barakat et al, 2017;Sen, 2017;Coelho et al, 2018;Wang et al, 2019;Orlowska et al, 2020;Broughton et al, 2020). The optimized conditions (temperature, light and humidity) can provide healthy donor plants.…”

Section: Growing Conditionsmentioning

confidence: 99%

“…The DH plant production methods offer an opportunity for quick selection of the recessive alleles. Furthermore, the in vitro anther culture can be combined with other value added methods such as marker assisted selection (MAS), QTL analyses, genetic transformation or induced mutation to achieve the desired goals of breeding and research (Chauhan and Khurana, 2011;Wessels and Botes, 2014;Barakat et al, 2017;Ren et al, 2017;Song et al, 2017;Shchukina et al, 2018;Tyrka et al, 2018;Shi et al, 2019;Testillano, 2019;Wajdzik et al, 2019;Bilichak et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Acta Biologica Cracoviensia s. Botanica

Lantos,

Pauk

2020

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Using in vitro androgenesis serves as a unique opportunity to produce doubled haploid (DH) plants in many species. More benefits of this biological phenomenon have kept these methods in the focus of fundamental research and crop breeding for decades. In common wheat (Triticum aestivum L.), in vitro anther culture is one of the most frequently applied DH plant production methods. The efficiency of in vitro wheat anther culture is influenced by many factors, such as the genotype, growing conditions, collection time, pre-treatments, and compositions of media and culture conditions. According to some critical review, the genotype dependency, low efficiency and albinism are mentioned as limitations of application of the anther culture method. However, some research groups have made significant efforts to diminish the effects of these bottlenecks. Due to the improvements, a well-established in vitro anther culture method can be an efficient tool in modern wheat breeding programs.

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“…Elimination of heterozygosity simplifies genome sequencing, reverse breeding, quantitative genetic research and discovering of recessive, dominant and deleterious mutations. That's why DHs are interesting objects of studies in many research areas including physiology, molecular biology, genetics and epigenetics (Maluszynski et al, 1996;Castillo et al, 2001;Touraev et al, 2001;Forster et al, 2007;Szarejko and Forster, 2007 and references therein;Dirks et al, 2009;Dunwell, 2010;Chauhan and Khurana, 2011;Ferrie and Möllers, 2011;Krzewska et al, 2012Krzewska et al, , 2017Marathi et al, 2012;Żur et al, 2014a,b, 2015aBarakat et al, 2017;Ren et al, 2017;Song et al, 2017;Shchukina et al, 2018;Tyrka et al, 2018;Nowicka et al, 2019;Shi et al, 2019;Testillano, 2019;Wajdzik et al, 2019;Bilichak et al, 2020;Malaga et al, 2020). Furthermore, DH technology combined with marker assisted selection (MAS) enables precise identification of plants with enhanced/silenced expression of even one gene of interest within the genome.…”

Section: Introductionmentioning

confidence: 99%

Proteins, Small Peptides and Other Signaling Molecules Identified as Inconspicuous but Possibly Important Players in Microspores Reprogramming Toward Embryogenesis

Dubas¹,

Żur²,

Moravčíková³

et al. 2021

Front. Sustain. Food Syst.

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In this review, we describe and integrate the latest knowledge on the signaling role of proteins and peptides in the stress-induced microspore embryogenesis (ME) in some crop plants with agricultural importance (i.e., oilseed rape, tobacco, barley, wheat, rice, triticale, rye). Based on the results received from the most advanced omix analyses, we have selected some inconspicuous but possibly important players in microspores reprogramming toward embryogenic development. We provide an overview of the roles and downstream effect of stress-related proteins (e.g., β-1,3-glucanases, chitinases) and small signaling peptides, especially cysteine—(e.g., glutathione, γ-thionins, rapid alkalinization factor, lipid transfer, phytosulfokine) and glycine-rich peptides and other proteins (e.g., fasciclin-like arabinogalactan protein) on acclimation ability of microspores and the cell wall reconstruction in a context of ME induction and haploids/doubled haploids (DHs) production. Application of these molecules, stimulating the induction and proper development of embryo-like structures and green plant regeneration, brings significant improvement of the effectiveness of DHs procedures and could result in its wider incorporation on a commercial scale. Recent advances in the design and construction of synthetic peptides–mainly cysteine-rich peptides and their derivatives–have accelerated the development of new DNA-free genome-editing techniques. These new systems are evolving incredibly fast and soon will find application in many areas of plant science and breeding.

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Doubled haploid wheat lines with high molecular weight glutenin alleles derived from microspore cultures

Cited by 10 publications

References 27 publications

Phenotypic and genetic diversity of doubled haploid bread wheat population and molecular validation for spike characteristics, end-use quality, and biofortification capacity

Phenotypic and genetic diversity of doubled haploid bread wheat population and molecular validation for spike characteristics, end-use quality, and biofortification capacity

Acta Biologica Cracoviensia s. Botanica

Proteins, Small Peptides and Other Signaling Molecules Identified as Inconspicuous but Possibly Important Players in Microspores Reprogramming Toward Embryogenesis

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