Double-network acrylamide hydrogel compositions adapted to achieve cartilage-like dynamic stiffness

Ronken, Sarah; Wirz, Dieter; Daniels, A. U.; Kurokawa, Takayuki; Gong, Jian Ping; Arnold, Markus

doi:10.1007/s10237-012-0395-6

Cited by 18 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While these silk hydrogels provided an excellent scaffold for chondrocyte attachment and cartilage matrix deposition, further improvement in the mechanical properties of these hydrogels is necessary to construct optimal load-bearing cartilage tissue constructs. Efforts in cartilage TE over the past few decades have improved hydrogel mechanics using methods such as chemical crosslinking [28, 29], double-network hydrogels [30, 31] and hydrogel interpenetrating scaffolds [32, 33]. While fiber reinforcement has also successfully enhanced the mechanical performance of hydrogel systems, little is known about the effects of fiber–gel composite systems on long-term cell viability and tissue development [34] [35].…”

Section: Introductionmentioning

confidence: 99%

Silk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair

et al. 2015

View full text Add to dashboard Cite

Cartilage tissue lacks an intrinsic capacity for self-regeneration due to slow matrix turnover, a limited supply of mature chondrocytes and insufficient vasculature. Although cartilage tissue engineering has achieved some success using agarose as a scaffolding material, major challenges of agarose-based cartilage repair, including non-degradability, poor tissue–scaffold integration and limited processing capability, have prompted the search for an alternative biomaterial. In this study, silk fiber–hydrogel composites (SF–silk hydrogels) made from silk microfibers and silk hydrogels were investigated for their potential use as a support material for engineered cartilage. We demonstrated the use of 100% silk-based fiber–hydrogel composite scaffolds for the development of cartilage constructs with properties comparable to those made with agarose. Cartilage constructs with an equilibrium modulus in the native tissue range were fabricated by mimicking the collagen fiber and proteoglycan composite architecture of native cartilage using biocompatible, biodegradable silk fibroin from Bombyx mori. Excellent chondrocyte response was observed on SF–silk hydrogels, and fiber reinforcement resulted in the development of more mechanically robust constructs after 42 days in culture compared to silk hydrogels alone. Thus, we demonstrate the versatility of silk fibroin as a composite scaffolding material for use in cartilage tissue repair to create functional cartilage constructs that overcome the limitations of agarose biomaterials, and provide a much-needed alternative to the agarose standard.

show abstract

Section: Introductionmentioning

confidence: 99%

Silk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Since the development of the DN hydrogel in 2003, Gong and Osada have recognized the comparability of DN properties to native cartilage and have consequentially studied their potential for cartilage replacement [5, 6], with other research groups following suit [7, 8]. More recently, they developed a method using a DN hydrogel to attempt in vivo articular cartilage regeneration without the use of encapsulated cells [9].…”

Section: Introductionmentioning

confidence: 99%

Tuning mechanical performance of poly(ethylene glycol) and agarose interpenetrating network hydrogels for cartilage tissue engineering

Rennerfeldt¹,

Renth²,

Talata³

et al. 2013

Biomaterials

View full text Add to dashboard Cite

Hydrogels are attractive for tissue engineering applications due to their incredible versatility, but they can be limited in cartilage tissue engineering applications due to inadequate mechanical performance. In an effort to address this limitation, our team previously reported the drastic improvement in the mechanical performance of interpenetrating networks (IPNs) of poly(ethylene glycol) diacrylate (PEG-DA) and agarose relative to pure PEG-DA and agarose networks. The goal of the current study was specifically to determine the relative importance of PEG-DA concentration, agarose concentration, and PEG-DA molecular weight in controlling mechanical performance, swelling characteristics, and network parameters. IPNs consistently had compressive and shear moduli greater than the additive sum of either single network when compared to pure PEG-DA gels with a similar PEG-DA content. IPNs withstood a maximum stress of up to 4.0 MPa in unconfined compression, with increased PEG-DA molecular weight being the greatest contributing factor to improved failure properties. However, aside from failure properties, PEG-DA concentration was the most influential factor for the large majority of properties. Increasing the agarose and PEG-DA concentrations as well as the PEG-DA molecular weight of agarose/PEG-DA IPNs and pure PEG-DA gels improved moduli and maximum stresses by as much as an order of magnitude or greater compared to pure PEG-DA gels in our previous studies. Although the viability of encapsulated chondrocytes was not significantly affected by IPN formulation, glycosaminoglycan (GAG) content was significantly influenced, with a 12-fold increase over a three-week period in gels with a lower PEG-DA concentration. These results suggest that mechanical performance of IPNs may be tuned with partial but not complete independence from biological performance of encapsulated cells.

show abstract

“…Higher porosity benefits cell diffusion, but it has poor mechanical properties. Thus, double-network (DN) hydrogels were developed, which can offer excellent mechanical properties—even with the water content exceeding 90%—and a dynamic stiffness value comparable to that of the swine meniscus [43]. Crosslinking methods involved in DN hydrogel preparation may be weak mechanical properties and speed degradation during implantation.…”

Section: Materials For Meniscal Scaffold and Cell Sourcementioning

confidence: 99%

An Overview of Scaffold Design and Fabrication Technology for Engineered Knee Meniscus

2017

View full text Add to dashboard Cite

Current surgical treatments for meniscal tears suffer from subsequent degeneration of knee joints, limited donor organs and inconsistent post-treatment results. Three clinical scaffolds (Menaflex CMI, Actifit ® scaffold and NUsurface ® Meniscus Implant) are available on the market, but additional data are needed to properly evaluate their safety and effectiveness. Thus, many scaffold-based research activities have been done to develop new materials, structures and fabrication technologies to mimic native meniscus for cell attachment and subsequent tissue development, and restore functionalities of injured meniscus for long-term effects. This study begins with a synopsis of relevant structural features of meniscus and goes on to describe the critical considerations. Promising advances made in the field of meniscal scaffolding technology, in terms of biocompatible materials, fabrication methods, structure design and their impact on mechanical and biological properties are discussed in detail. Among all the scaffolding technologies, additive manufacturing (AM) is very promising because of its ability to precisely control fiber diameter, orientation, and pore network micro-architecture to mimic the native meniscus microenvironment.

show abstract

Double-network acrylamide hydrogel compositions adapted to achieve cartilage-like dynamic stiffness

Cited by 18 publications

References 24 publications

Silk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair

Silk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair

Tuning mechanical performance of poly(ethylene glycol) and agarose interpenetrating network hydrogels for cartilage tissue engineering

An Overview of Scaffold Design and Fabrication Technology for Engineered Knee Meniscus

Contact Info

Product

Resources

About