Dot-ELISA vs. PCR of Fine Needle Aspirates of Tuberculous Lymphadenitis

Thyroid tuberculosis – role of PCR in diagnosis of a rare entity

“…PCR amplification of a 123 bp fragment of IS6110 is specific for M. tuberculosis complex 23 . The sensitivity and specificity of PCR are approximately 88.8% and 100%, respectively, for detection of Mycobacterial infection in lymph node aspirates 24,25 . The sensitivity of PCR depends on the efficiency of the DNA extraction procedure.…”

Nucleic Acid Amplification of Mycobacterium tuberculosis Complex DNA from Archival Fine Needle Aspiration Smear Scrapings vs. Fresh Fine Needle Aspirates of Tuberculous Lymphadenitis

“…The material for the study included the archival FNA smears of 33 cytologically diagnosed cases of TB (classified into 7 cytomorphologic categories), subsequently confirmed using an extended gold standard, 6 13 non-TB control smears and 5 known Ziehl-Neelsen (ZN)-stained, acid-fast bacilli (AFB)-positive and NAA-positive TB sputum control smears. NAA from the direct FNA material from the same cases had been performed 1 year earlier, when the material from needle washings was used for NAA and smears were made from a separate prick for cytomorphologic examination.…”

Drug Resistant Mycobacterium tuberculosis Complex Isolates among Patients with Tuberculous Lymphadenitis

“…Table 2: Baseline characteristics, cytomorphological patterns and circulating mycobacterial DNA of the study patients Fine Needle Aspiration Cytology (FNAC) has proved very valuable in the diagnosis of tuberculous Lymphadenitis where mycobacterial infections are endemic and when cytomorphological patterns proved to extremely concordant with microbiological and molecular present of mycobacteria. However, it has several limitations, especially in the absence of demonstrable AFB, but FNAC provides at least material for PCR in an easy and moderately invasive manner [12][13][14]. Recently PCR-based techniques were applied in the diagnosis of tuberculous lymphadenitis [14,15].…”

“…Granulomatous, granulomatous/necrotizing tuberculous lymphadenitis,reactive lymphadenopathy and secondary malignant deposit. DNA was extracted from lysates using the phenol chloroform method (PCI) as described by Maniatis et al [11][12][13][14][15]. Following DNA extraction, all lysate samples were subjected to PCR amplification to identify M. tuberculosis complex from other bacteria using a set of forward and reverse primers were used to amplify the target gene IS6110 ( Table 1).…”