Dose optimization of decellularized skeletal muscle extracellular matrix hydrogels for improving perfusion and subsequent validation in an aged hindlimb ischemia model

Hernandez, Melissa J.; Zelus, Emma I; Spang, Martin T.; Braden, Rebecca L.; Christman, Karen L.

doi:10.1039/c9bm01963d

Cited by 22 publications

(21 citation statements)

References 53 publications

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“…Transcriptome analysis indicated that the skeletal muscle hydrogel caused a shift in the inflammatory response, decreased cell death as well as increased blood vessel and muscle development. Finally, Hernandez et al showed that the improvements in blood perfusion seen with this hydrogel was dependent on the concentration of the ECM 138 . ECM hydrogels have the potential to improve muscle regeneration after ischemic injury; however, further studies need to be done to confirm this.…”

Section: Biomimetic Materialsmentioning

confidence: 94%

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Promoting endogenous repair of skeletal muscle using regenerative biomaterials

Carleton

Sefton

2021

J Biomedical Materials Res

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Skeletal muscles normally have a remarkable ability to repair themselves; however, large muscle injuries and several myopathies diminish this ability leading to permanent loss of function. No clinical therapy yet exists that reliably restores muscle integrity and function following severe injury. Consequently, numerous tissue engineering techniques, both acellular and with cells, are being investigated to enhance muscle regeneration. Biomaterials are an essential part of these techniques as they can present physical and biochemical signals that augment the repair process. Successful tissue engineering strategies require regenerative biomaterials that either actively promote endogenous muscle repair or create an environment supportive of regeneration. This review will discuss several acellular biomaterial strategies for skeletal muscle regeneration with a focus on those under investigation in vivo. This includes materials that release bioactive molecules, biomimetic materials and immunomodulatory materials.

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Section: Biomimetic Materialsmentioning

confidence: 94%

“…Finally, Hernandez et al showed that the improvements in blood perfusion seen with this hydrogel was dependent on the concentration of the ECM. 138 ECM hydrogels have the potential to improve muscle regeneration after ischemic injury; however, further studies need to be done to confirm this.…”

Section: Biomimetic Materialsmentioning

confidence: 99%

Promoting endogenous repair of skeletal muscle using regenerative biomaterials

Carleton

Sefton

2021

J Biomedical Materials Res

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“…These cell-containing hydrogels can then be injected for minimally invasive delivery into irregular spaces, [68][69][70] applied topically or used for 3D printing or electrospinning. 57,71 Some of the medical conditions for which decellularised ECM-based hydrogels have been investigated include type 1 diabetes, 70 myocardial infarction by replacing damaged cardiac tissue, 68,69 peripheral artery disease, 72 skin wound healing, 73 keratoconus by bioprinting a corneal stromal substitute, 71 acute liver failure, 74 chronic fibrotic diseases 75 and inflammatory bowel disease. 76 The impact of the decellularisation method on the ECM-derived hydrogel has been shown by several studies.…”

Section: Hydrogels With Decellularised Matricesmentioning

confidence: 99%

Decellularised scaffolds: just a framework? Current knowledge and future directions

et al. 2020

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The use of decellularised matrices as scaffolds offers the advantage of great similarity with the tissue to be replaced. Moreover, decellularised tissues and organs can be repopulated with the patient’s own cells to produce bespoke therapies. Great progress has been made in research and development of decellularised scaffolds, and more recently, these materials are being used in exciting new areas like hydrogels and bioinks. However, much effort is still needed towards preserving the original extracellular matrix composition, especially its minor components, assessing its functionality and scaling up for large tissues and organs. Emphasis should also be placed on developing new decellularisation methods and establishing minimal criteria for assessing the success of the decellularisation process. The aim of this review is to critically review the existing literature on decellularised scaffolds, especially on the preparation of these matrices, and point out areas for improvement, finishing with alternative uses of decellularised scaffolds other than tissue and organ reconstruction. Such uses include three-dimensional ex vivo platforms for idiopathic diseases and cancer modelling.

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“…Moreover, decellularized tissue ECM has robust potential to improve tissue regeneration through native growth factors, cytokines, and chemokines. 110,111 Further applications with engineered tyrosinase and polyphenolic hydrogel and decellularized tissue ECM will be organized for the next generation of tissue engineering.…”

Section: Conclusion and Future Perspectives In Tyrosinase-mediated Tissue Engineeringmentioning

confidence: 99%

Tyrosinase‐mediated hydrogel crosslinking for tissue engineering

Choi

Ahn

Kim

2021

J of Applied Polymer Sci

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Hydrogel system based on enzyme-mediated mild crosslinking reaction has been a promising approach in tissue engineering. Inspired by skin melanin synthesis and marine mussel adhesion, tyrosinase-mediated hydrogel crosslinking has been exploited as cell-friendly reactions and explicit reaction mechanisms. Hydrogel prepared by tyrosinase exhibits appealing properties as a dynamic scaffold for cell delivery and as a bioink for 3D bioprinting. Recapitulating the structure of the native extracellular matrix (ECM), innovative tyrosinase-mediated hydrogel crosslinking has now shifted to the field of translational medicine. Biomimetic hydrogel with in situ tyrosinase crosslinking can be efficiently and easily applicable to the disease model for therapeutic purposes. In this review, we will discuss a broad range of tyrosinase-mediated tissue engineering from the specific mechanism of tyrosinase reaction and designing a strategy for tyrosinase-mediated hydrogel crosslinking to dynamic applications in tissue engineering. In addition, we report the current challenges and future perspectives for the translational applications.

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Dose optimization of decellularized skeletal muscle extracellular matrix hydrogels for improving perfusion and subsequent validation in an aged hindlimb ischemia model

Abstract: An optimal concentration of a decellularized skeletal muscle extracellular matrix hydrogel was eventuated for eventual clinical translation.

Cited by 22 publications

References 53 publications

Promoting endogenous repair of skeletal muscle using regenerative biomaterials

Promoting endogenous repair of skeletal muscle using regenerative biomaterials

Decellularised scaffolds: just a framework? Current knowledge and future directions

Tyrosinase‐mediated hydrogel crosslinking for tissue engineering

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