Tyrosinase‐mediated hydrogel crosslinking for tissue engineering

Choi, Su‐Mi; Ahn, Hyerin; Kim, Su Hwan

doi:10.1002/app.51887

Cited by 28 publications

(18 citation statements)

References 108 publications

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“…These limitations generated by crosslinking methods can be overcome by using enzymes to form covalently crosslinked hydrogels. Enzyme-mediated crosslinking by tyrosinases [ 16 ], transferases [ 17 ] or peroxidases [ 18 ] have proven their efficiency and therefore, they have attracted increasing attention for application in polymer hydrogel synthesis due to the environmentally-friendly process and the possibility of obtaining biomaterials with extracellular matrix-mimicking properties. Transglutaminase (TGase), a widely present enzyme in nature, has been demonstrated to catalyze the formation of isopeptide bonds between proteins by mimicking in vivo biosynthetic processes, which can significantly improve protein gelation [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Rusu

Niţă

Simiónescu³

et al. 2023

Polymers

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Development of natural protein-based hydrogels with self-healing performance and tunable physical properties has attracted increased attention owing to their wide potential not only in the pharmaceutical field, but also in wounds management. This work reports the development of a versatile hydrogel based on enzymatically-crosslinked gelatin and nanogels loaded with amoxicillin (Amox), an antibiotic used in wound infections. The transglutaminase (TGase)-crosslinked hydrogels and encapsulating nanogels were formed rapidly through enzymatic crosslinking and self-assembly interactions in mild conditions. The nanogels formed through the self-assemble of maleoyl-chitosan (MAC5) and polyaspartic acid (PAS) may have positive influence on the self-healing capacity and drug distribution within the hydrogel network through the interactions established between gelatin and gel-like nanocarriers. The physicochemical properties of the enzymatically-crosslinked hydrogels, such as internal structure, swelling and degradation behavior, were studied. In addition, the Amox release studies indicated a rapid release when the pH of the medium decreased, which represents a favorable characteristic for use in the healing of infected wounds. It was further observed through the in vitro and in vivo biocompatibility assays that the optimized scaffolds have great potential to be used as wound dressings.

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Section: Introductionmentioning

confidence: 99%

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Rusu

Niţă

Simiónescu³

et al. 2023

Polymers

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“…(3) Mild coating conditions: the surface coating proceeds under pure aqueous and neutral pH conditions (pH 6–7) without the use of strong chemical oxidants. The coating solution comprising tyrosinase and oxidized species of Tyr-SB is cytocompatible to living cells, supporting that the current coating method would serve as a useful tool in cell encapsulation, tissue engineering, and regenerative medicine . Meanwhile, relatively small surface dimensions up to the centimeter scale rather than large surface areas are preferred because our coating system requires enzyme and synthetic Tyr-SB to trigger surface coating.…”

Section: Discussionmentioning

confidence: 90%

Antifouling Surface Coating on Various Substrates by Inducing Tyrosinase-Mediated Oxidation of a Tyrosine-Conjugated Sulfobetaine Derivative

et al. 2022

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Inspired by the melanogenesis occurring in nature, we report tyrosinase-mediated antifouling surface coating by synthesizing a tyrosine-conjugated sulfobetaine derivative (Tyr-SB). Synthetic Tyr-SB contains zwitterionic sulfobetaine and tyrosine, whose phenolic amine group acts as a dormant coating precursor. In contrast to catecholamine derivatives, tyrosine derivatives are stable against auto-oxidation and are enzymatically oxidized only in the presence of tyrosinase to initiate melanin-like oxidation. When the surface of interest was applied during the course of Tyr-SB oxidation, a superhydrophilic poly(Tyr-SB) film was coated on the surfaces, thereby showing antifouling performance against proteins or adherent cells. Because the oxidation of Tyr-SB occurred under mild aqueous conditions (pH 6−7) without the use of any chemical oxidants, such as sodium periodate or ammonium persulfate, we anticipate that the coating method described herein will serve as a biocompatible tool in the field of biosensors, cell surface engineering, and medical devices, whose interfaces differ in chemistry.

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“…Nonetheless, its bioprinting is difficult because of poor gelation kinetics and lack of suitable rheological proprieties [191], which can be circumvented by mixing with more rapidly gelling components [192]. Other examples of enzymes used for 3D printing are microbial transglutaminase [193,194], tyrosinase [195,196], and thrombin [197,198]. The latter is usually applied to blood-derived materials, as these possess fibrinogen in their composition allowing for their conversion into insoluble fibrin forming a stable gel [123,199].…”

Section: Enzyme-basedmentioning

confidence: 99%

Biomaterials of human source for 3D printing strategies

et al. 2023

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Three-dimensional printing has risen in recent years as a promising approach that fast-tracked the biofabrication of tissue engineering constructs that most resemble utopian tissue/organ replacements for precision medicine. Additionally, by using human-sourced biomaterials engineered towards optimal rheological proprieties of extrudable inks, the best possible scaffolds can be created. These can encompass native structure and function with a low risk of rejection, enhancing overall clinical outcomes; and even be further optimized by engaging in information- and computer-driven design workflows. This paper provides an overview of the current efforts in achieving ink’s necessary rheological and print performance proprieties towards biofabrication from human-derived biomaterials. The most notable step for arranging such characteristics to make biomaterials inks are the employed crosslinking strategies, for which examples are discussed. Lastly, this paper illuminates the state-of-the-art of the most recent literature on already used human-sourced inks; with a final emphasis on future perspectives on the field.

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Tyrosinase‐mediated hydrogel crosslinking for tissue engineering

Cited by 28 publications

References 108 publications

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Antifouling Surface Coating on Various Substrates by Inducing Tyrosinase-Mediated Oxidation of a Tyrosine-Conjugated Sulfobetaine Derivative

Biomaterials of human source for 3D printing strategies

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