Dose-Dependent Minimisation of Cytogenetic Toxicity of Endosulfan(an Organochlorine Pesticide) by Vitamin C.

Khan, Parimal Kumar; Sinha, S. P.

doi:10.1508/cytologia.57.217

Cited by 6 publications

(4 citation statements)

References 22 publications

(19 reference statements)

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“…The present observations strongly supports the earlier study demonstrated the ameliorating effects vitamin C (L-ascorbic acid) in modulating the genotoxicity of pesticides. [26] The results of the present investigation also support the findings of Semercioz et al ,[39] who have used Melatonin, as free radical scavenger, thereby suggesting that the atrazine induces its toxic manifestations possibly through free radical generation and scavengers thereof like vitamin E (100 μmolL -1 ) ameliorate these toxic effects.…”

Section: Discussionsupporting

confidence: 91%

“…vitamin E) can increase their ability to protect against lipid peroxidation. [25] The protecting effects of high doses level 20 and 40 mg/kg body wt/day of vitamin C (L-ascorbic acid) in modulating the genotoxicity of pesticides in murine bone marrow cells,[26–28] and in primary spermatocytes have been observed. [29] Positive effect of vitamin C (ascorbic acid) in preventing the degenerating effects of endosulphan in Capra hircus in vitro have been documented.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant attenuation of atrazine induced histopathological changes in testicular tissue of goat in vitro

2012

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During the present investigation the effect of α-tocopherol (100 μmolL-1) in prevention of testicular toxicity induced by atrazine in goat Capra hircus have been analyzed. Vitamin E (α-tocopherol) at dose level 100 μmolL-1 provides attenuation over the histopathological changes generated by pesticide atrazine (100 nmolml-1). Small pieces (approximately 1mm3) of testicular tissue were divided into three groups (one control group + two experimental groups). Experimental group (A) was supplemented with 100 nmolml-1 concentration of atrazine and experimental group (B) was supplemented with 100 nmolml-1 atrazine and 100 μmolL-1 concentrations of vitamin E (α-Tocopherol) and harvesting was carried out after 1, 4 and 8 hrs of exposure. Control was run along with all the experimental groups. In the experimental group (A) treated with atrazine at dose level 100 nmolml-1, revealed histomorphological alterations in the seminiferous tubule. After one hour of exposure duration small vacuoles in cytoplasm of the Sertoli cells and spermatogonia were observed. Chromolysis at pycnosis were also noticed in the spermatogonia and spermatids. In the experimental group (B) exposed with atrazine and simultaneously supplemented with Vitamin E also showed degeneration but it was milder as compared with experimental group treated with atrazine without antioxidant. Atrazine exposure induced a decline in diameter of spermatocytes from 10.51 ± 0.2052 μm in control to 7.915 ± 0.2972, 7.5 ± 0.211 and 7.14 ± 0.225 μm after exposure of 1, 4 and 8 hrs respectively but in case of atrazine supplemented with vitamin E [experimental group (B)], there was less decline in cell diameter that was 8.5 ± 0.1865, 8.1 ± 0.1201 and 7.8 ± 0.2066μm after exposure of 1, 4 and 8 hrs respectively. The result demonstrated that vitamin E delays the degenerative changes induced by atrazine.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Antioxidant attenuation of atrazine induced histopathological changes in testicular tissue of goat in vitro

2012

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“…In continuation with our previous experiments [4,5], the present ones have been undertaken to explore the extent of amelioration provided by higher doses of vitamin C against the cytogenetic toxicity of pesticide formulations. Fortunately, vitamin C has practically no toxic effects of its own even when administered at comparatively higher doses [ 1 1,121.…”

Section: Introductionmentioning

confidence: 93%

“…Use of vitamin C (L-ascorbic acid) as an antimutagen has been shown to be a promising solution to circumvent the genotoxicity of pesticides in vivo [1][2][3][4][5][6]. The recommended dietary allowances (RDA) for this vitamin to prevent scurvy is 45-60 mg/day for a healthy person [7].…”

Section: Introductionmentioning

confidence: 99%

Impact of higher doses of vitamin C in modulating pesticide genotoxicity

Khan¹,

Sinha²

1994

Teratog Carcinog Mutagen

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The modulatory effect of higher doses of vitamin C (ascorbic acid) on the genotoxicity of the three pesticides (endosulfan, phosphamidon, and mancozeb) was assessed in the in vivo micronucleus test in Swiss albino mice. Concurrent administration of the vitamin in a dose (20 mg/kg bwt/day) equivalent to double the human therapeutic one, along with each of the three pesticides, was most effective as an antimutagen. The therapeutic dose (10 mg/kg bwt/day) was comparatively less so, and the quadruple (40 mg/kg bwt/day) of it did not show any further amelioration.

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