Dose-dependent in-vivo toxicity assessment of silver nanoparticle in Wistar rats

Tiwari, Dhermendra K.; Jin, Takashi; Behari, J.

doi:10.3109/15376516.2010.529184

Cited by 232 publications

(142 citation statements)

References 54 publications

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“…Our study aimed to analyze the toxicity of AgNPs at different doses in a murine model and to study the application of AgNPs for targeting murine fibrosarcoma. Tiwari et al 19 in 2011 studied the toxicity of AgNPs in the doses of 4 mg, 10 mg, 20 mg and 40 mg/kg b.w. in Wistar rats and concluded that doses near 4 mg/kg body weight were best suited for the therapeutic application.…”

Section: Discussionmentioning

confidence: 99%

“…in Wistar rats and concluded that doses near 4 mg/kg body weight were best suited for the therapeutic application. 19 The low dose range of 1-10 mg/kg b.w. has thus been prioritized to avoid the higher doses that would have been obviously toxic.…”

Section: Discussionmentioning

confidence: 99%

“…in rats showed liver toxicity. 19 Moreover, in in vitro and in vivo studies conducted by Sriram et al, 20 it was shown that AgNPs have antitumor properties against Dalton's lymphoma ascites tumor model. Thus, recent findings suggest that there is a relationship between AgNPs and the immune system, however, there are almost no reports on the AgNP immunomodulation of tumor-bearing mice.…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory properties of silver nanoparticles contribute to anticancer strategy for murine fibrosarcoma

et al. 2015

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The use of nanotechnology in nanoparticle-based cancer therapeutics is gaining impetus due to the unique biophysical properties of nanoparticles at the quantum level. Silver nanoparticles (AgNPs) have been reported as one type of potent therapeutic nanoparticles. The present study is aimed to determine the effect of AgNPs in arresting the growth of a murine fibrosarcoma by a reductive mechanism. Initially, a bioavailability study showed that mouse serum albumin (MSA)-coated AgNPs have enhanced uptake; therefore, toxicity studies of AgNP-MSA at 10 different doses (1-10 mg/kg b.w.) were performed in LACA mice by measuring the complete blood count, lipid profile and histological parameters. The complete blood count, lipid profile and histological parameter results showed that the doses from 2 to 8 mg (IC 50 : 6.15 mg/kg b.w.) sequentially increased the count of leukocytes, lymphocytes and granulocytes, whereas the 9-and 10-mg doses showed conclusive toxicity. In an antitumor study, the incidence and size of fibrosarcoma were reduced or delayed when murine fibrosarcoma groups were treated by AgNP-MSA. Transmission electron micrographs showed that considerable uptake of AgNP-MSA by the sentinel immune cells associated with tumor tissue and a morphologically buckled structure of the immune cells containing AgNP-MSA. Because the toxicity studies revealed a relationship between AgNPs and immune function, the protumorigenic cytokines TNF-a, IL-6 and IL-1b were also assayed in AgNP-MSA-treated and non-treated fibrosarcoma groups, and these cytokines were found to be downregulated after treatment with AgNP-MSA. Cellular & Molecular Immunology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory properties of silver nanoparticles contribute to anticancer strategy for murine fibrosarcoma

et al. 2015

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“…Tiwari et al (36) assessed increased single and double DNA breakage in rats after intravenous injection of 40 mg kg -1 bw of Ag NPs. In the second in vivo study, zebrafi sh were treated with oral Ag NPs (5 nm to 20 nm), which resulted in high levels of γ-H2AX -a marker for double DNA strand breaks.…”

Section: Genotoxic Potential Of Silver Npsmentioning

confidence: 99%

Genotoxicity of Metal Nanoparticles: Focus on In Vivo Studies

Klien

Godnič‐Cvar

2012

Archives of Industrial Hygiene and Toxicology

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With increasing production and application of a variety of nanomaterials (NMs), research on their cytotoxic and genotoxic potential grows, as the exposure to these nano-sized materials may potentially result in adverse health effects. In large part, indications for potential DNA damaging effects of nanoparticles (NPs) originate from inconsistent in vitro studies. To clarify these effects, the implementation of in vivo studies has been emphasised. This paper summarises study results of genotoxic effects of NPs, which are available in the recent literature. They provide indications that some NP types cause both DNA strand breaks and chromosomal damages in experimental animals. Their genotoxic effects, however, do not depend only on particle size, surface modifi cation (particle coating), and exposure route, but also on exposure duration. Currently available animal studies may suggest differing mechanisms (depending on the duration of exposure) by which living organisms react to NP contact. Nevertheless, due to considerable inconsistencies in the recent literature and the lack of standardised test methods -a reliable hazard assessment of NMs is still limited. Therefore, international organisations (e.g. NIOSH) suggest utmost caution when potential exposure of humans to NMs occurs, as long as evidence of their toxicological and genotoxic effect(s) is limited.

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“…Apart from these, cellular porous permeability was found indirectly proportional to volume of experimental colloid and time of exposure [7]. Thorny tiny silver nano particle with breadth and diameter below 1.55 to 19.57 nm range after specific experimental application found to be initiative of certain chronic kidney inflammatory diseases, [8] nano metal of this category also become barrier in proper development of viruses, fungus and certain dangerous parasites. Small size nano particles tremendously heals damaged tissue after hurts from fire injury, traumatic injury and certain diabetic and diseased wound but the negative feedback is after smooth absorption from skin it causes indirect damage to internal organs like liver and kidney followed by chronic deposition [9].…”

Section: Size Dependant Nephrotoxicity Focus In Indirect Responsementioning

confidence: 99%

Small Size Silver Nanoparticle’s Corrosive and Hazardous Manifestations on Mature and Developing Kidney Following Accumulation in Pregnant Mice and Offspring’s after Serial Oral Bolus Experimental Application: A New Chapter in Teratogenicity and Toxicity Search

Prakash¹,

Singh²,

Pani³

2017

J Cytol Histol

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The most important and worldwide utilized nanomaterial is silver metal nanoparticle which after synthesis changed to colloidal or powdered form. After successful characterization different sizes of these particles measured if intermixed and confirmed to different size ranges. Different sizes segregates into various size groups. Specific small or large size silver in powder form also readily available in market for various applications and experiments after biological or herbal synthesis and characterization. 1 to 20 nm size ranges are grouped under inconsiderable or smaller size range whereas 20 to 100 nm size ranges or above sizes are grouped under considerable or bigger size range. Following serial oral ingestion for experiment, aqueous and magnetic stirred synthesized AgNps of less than 20 nm of null account size accumulate in mature and developing kidneys of pregnant Swiss Albino mice and their freshly born fetuses after passing through blood vessels of pregnant mice and after absorption from gastro intestinal tract and accumulate in mature kidney due to trap inside. These particles also crosses blood placental barrier reaches fetus body in womb and accumulate in developing kidney due to same mechanism. These particles also surprisingly execute sex specific corrosive manifestation. We have examined the corrosive and hazardous effect of 10 gestational days (5 to 15 gestational days) serial oral bolus ingestion of 2.75 to 18.5 nm size range AgNps colloidal solution in a lower dose of 0.35 (1), 0.55 (2) mg/kg. b.w, in an in between dose of 4 (3), 8 (4) mg/ kg. b.w. and in a higher dose of 12 (5) and 16 (6) mg/kg. b.w. treatment on mature and developing right and left kidneys of pregnant mice and fetuses. Groups were designated as 1(Control); 2, 3, 4, 5 and 6 (Treated). At lower dose we report negligible histological findings and at higher bolus dose we report marked histological findings. This particular size range group silver nano particles (AgNps) treatment turns into insignificant reduction in kidney weight and height at higher dose, with some hemorrhagic focuses along with histological alterations. Predominantly agenesis and severe atrophy of renal corpuscles, increase of urinary space, generalized anasarca of podocytes, degeneration and dysmorphology with congestion of proximal convoluted tubules and distal convoluted tubules, loss of brush border membranes and damage of squamous epithelium of loop of Henley with severe congestion in medullary rays and collecting ducts are observed in routine histology. The in between dose demonstrated in between results. These manifestations are found quite same to chronic and acute renal failure with hematuria. All these alterations, in prolong sustain, could land up to beginning of apoptosis and progress of cell necrosis in these vital organs.

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Dose-dependent in-vivo toxicity assessment of silver nanoparticle in Wistar rats

Cited by 232 publications

References 54 publications

Immunomodulatory properties of silver nanoparticles contribute to anticancer strategy for murine fibrosarcoma

Immunomodulatory properties of silver nanoparticles contribute to anticancer strategy for murine fibrosarcoma

Genotoxicity of Metal Nanoparticles: Focus on In Vivo Studies

Small Size Silver Nanoparticle’s Corrosive and Hazardous Manifestations on Mature and Developing Kidney Following Accumulation in Pregnant Mice and Offspring’s after Serial Oral Bolus Experimental Application: A New Chapter in Teratogenicity and Toxicity Search

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