Dosage and Passage Dependent Neuroprotective Effects of Exosomes Derived from Rat Bone Marrow Mesenchymal Stem Cells: An In Vitro Analysis

Venugopal, Chaitra; Shamir, Christopher; Senthilkumar, Sivapriya; Babu, Janitri Venkatachala; Sonu, Peedikayil Kurien; Nishtha, Kusum Jain; Kiranmai, S.; Shobha, KL; Dhanushkodi, Anandh

doi:10.2174/1566523218666180125091952

Cited by 31 publications

(30 citation statements)

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“…The smaller the generation, the stronger neuroprotective effect of the exosomes is. Low-dose exosomes could inhibit neuronal injury through antiapoptotic effects and oxidation, while high-dose exosomes had the opposite effect on neurons (Venugopal et al, 2017).…”

Section: Overview Of Exosomesmentioning

confidence: 99%

Progress of Research on Exosomes in the Protection Against Ischemic Brain Injury

et al. 2019

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Exosomes, as a type of extracellular vesicle (EV), are lipid bilayer vesicles 20–100 nm in diameter that can cross the blood-brain barrier. Exosomes are important transport vesicles in the human body that participate in many conduction pathways and play an important physiological role. Because of their high biocompatibility and low immunogenicity and toxicity, exosomes have attracted increasing attention as an attractive drug delivery system. This article reviews the relevant studies that have shown that exosomes play an important role in protective mechanisms against ischemic brain injury.

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Section: Overview Of Exosomesmentioning

confidence: 99%

Progress of Research on Exosomes in the Protection Against Ischemic Brain Injury

et al. 2019

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“…We hypothesized that these anti-apoptotic and obtained with 5 μg/mL (Xiao et al, 2016). Significant results were also obtained with low concentrations varying from 0.05 μg/mL to 0.4 μg/mouse of exosomes (Tan et al, 2014;Venugopal et al, 2017). Thus, we aimed to test the effects of low concentrations of cAmMSC-exo in our study, to give more insights about the effects of exosomes at low dosages.…”

Section: Resultsmentioning

confidence: 99%

“…The absence of beneficial effects of cAmMSC-exo could be explained by the cell type and thus the factors they secreted, the possible lack of exosome uptake from spermatozoa cells or by eventual cryo-damages induced on the exosomes, as they can also be affected by the cryopreservation process, resulting in a reduced biological activity (Bosch et al, 2016). Interestingly, exosomes can act on the same tissue differently depending on their concentration and the cell passaging (Venugopal et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Canine amniotic membrane derived mesenchymal stem cells exosomes addition in canine sperm freezing medium

Mahiddine

Qamar

Kim

2020

J Anim Reprod Biotechnol

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Exosomes have been discovered in 1983 (Pan and Johnstone, 1983) and are defined as 40-100 nm extracellular vesicles originating from the fusion of multi-vesicular bodies, also referred to as late endosomes, with the plasma membrane. They are known to mediate cell-to-cell communication as they can carry proteins, nucleic acids, and lipids, providing through that cargo a paracrine effect to the recipient tissues or cells (Mitchell et al., 2019). Thus, stem cell-derived exosomes have been used as a shuttle in cell-free therapies for the treatment of different conditions such as kidney diseases (Dorronsoro and Robbins, 2013) or osteoarthritis (Zhang et al., 2019). Among stem cell types, perinatal tissue-derived stem cells are a good alternative to embryonic stem cells as they are obtained without major ethical issues since they come from discarded fetal annexa, but the use of their secretome is still not common as there are over 30 clinical trials using perinatal tissue-derived stem cells but less than 10 trials using their derived exosomes (https://clinicaltrials.gov/). Among the perinatal tissues, the amniotic membrane is a good source of mesenchymal stem cells (Alviano et al., 2007) but the use of these stem cells or their derived exosomes is not as spread out as the other stem cells types, although they have the same properties and their secretome is as rich in growth and anti-apoptotic factors (An et al., 2017).

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“…EVs reflect the actual status of the parental cell, which is very susceptible to minor fluctuations in internal and external factors, including culture medium [65], passage number [66,67], seeding density [66], serum concentrations [68] and oxygen levels [69,70], which could explain inconsistencies between EV studies. Culturing MSCs under hypoxic conditions has indeed been observed to increase their EV production and functional effects on endothelial cell proliferation and tube formation [69,70].…”

Section: Discussionmentioning

confidence: 99%

Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles

Merckx

Hosseinkhani

Kuypers

et al. 2020

Cells

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Blood vessel formation or angiogenesis is a key process for successful tooth regeneration. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) possess paracrine proangiogenic properties, which are, at least partially, induced by their extracellular vesicles (EVs). However, the isolation of BM-MSCs is associated with several drawbacks, which could be overcome by MSC-like cells of the teeth, called dental pulp stromal cells (DPSCs). This study aims to compare the angiogenic content and functions of DPSC and BM-MSC EVs and conditioned medium (CM). The angiogenic protein profile of DPSC- and BM-MSC-derived EVs, CM and EV-depleted CM was screened by an antibody array and confirmed by ELISA. Functional angiogenic effects were tested in transwell migration and chicken chorioallantoic membrane assays. All secretion fractions contained several pro- and anti-angiogenic proteins and induced in vitro endothelial cell motility. This chemotactic potential was higher for (EV-depleted) CM, compared to EVs with a stronger effect for BM-MSCs. Finally, BM-MSC CM, but not DPSC CM, nor EVs, increased in ovo angiogenesis. In conclusion, we showed that DPSCs are less potent in relation to endothelial cell chemotaxis and in ovo neovascularization, compared to BM-MSCs, which emphasizes the importance of choice of cell type and secretion fraction for stem cell-based regenerative therapies in inducing angiogenesis.

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Dosage and Passage Dependent Neuroprotective Effects of Exosomes Derived from Rat Bone Marrow Mesenchymal Stem Cells: An In Vitro Analysis

Abstract: Our study suggests that adult stem cells derived exosomes could be a potential therapeutic agent to confer neuroprotection in neurodegenerative diseases like Alzheimer's disease.

Cited by 31 publications

References 0 publications

Progress of Research on Exosomes in the Protection Against Ischemic Brain Injury

Progress of Research on Exosomes in the Protection Against Ischemic Brain Injury

Canine amniotic membrane derived mesenchymal stem cells exosomes addition in canine sperm freezing medium

Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles

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