“…A small incision was made in the chick skin to expose the surface of the superficial masseter muscle. Then 1-5µlit of 40% HRP (type VI-Sigma) dissolved in sterile saline was injected slowly into the 2-5 loci above as well as under the parotid duct in the left masseter using a Hamilton syringe as demonstrated by Kawagishi et al (Kawagishi et al, 1992). After each injection the needle was left in situ for 1 min to avoid backflow of the injected HRP, following which the needle was removed, the injection sites were cleaned with sterile saline, and the opening was sutured.…”
Section: Intramasseter Hrp Injectionmentioning
confidence: 99%
“…Thus in this chapter the morphological features of the developing masseter motoneurons labeled by injection of HRP into the superficial masseter muscle were analyzed in normal and congenital hypothyroid rat's offspring. HRP retrograde reaction p r o d u c t i s o b s e r v e d a s d a r k b l u e i n t r a c e llular granules varying in quantity from motoneuron to motoneuron even in the same trigeminal motor nucleus (Kawagishi et al, 1992). As hypothyroidism reduces neuronal process growth, synaptogenesis, axonal transport velocity (Biesiada et al, 1996;Stein et al, 1991) and neurotransmitter synthesis (Barakat-Walter et al, 2000;Behzadi & Ganji, 2005), it was of especial interest to investigate the alteration in the morphological characteristics of masseter motoneurons as well as HRP uptake and transport from the neuromuscular junction.…”
“…A small incision was made in the chick skin to expose the surface of the superficial masseter muscle. Then 1-5µlit of 40% HRP (type VI-Sigma) dissolved in sterile saline was injected slowly into the 2-5 loci above as well as under the parotid duct in the left masseter using a Hamilton syringe as demonstrated by Kawagishi et al (Kawagishi et al, 1992). After each injection the needle was left in situ for 1 min to avoid backflow of the injected HRP, following which the needle was removed, the injection sites were cleaned with sterile saline, and the opening was sutured.…”
Section: Intramasseter Hrp Injectionmentioning
confidence: 99%
“…Thus in this chapter the morphological features of the developing masseter motoneurons labeled by injection of HRP into the superficial masseter muscle were analyzed in normal and congenital hypothyroid rat's offspring. HRP retrograde reaction p r o d u c t i s o b s e r v e d a s d a r k b l u e i n t r a c e llular granules varying in quantity from motoneuron to motoneuron even in the same trigeminal motor nucleus (Kawagishi et al, 1992). As hypothyroidism reduces neuronal process growth, synaptogenesis, axonal transport velocity (Biesiada et al, 1996;Stein et al, 1991) and neurotransmitter synthesis (Barakat-Walter et al, 2000;Behzadi & Ganji, 2005), it was of especial interest to investigate the alteration in the morphological characteristics of masseter motoneurons as well as HRP uptake and transport from the neuromuscular junction.…”
“…Although a lot of useful information has been obtained from structural (17), ultrastructural (18), quantitative (19,20) and clinical (11) studies, there are few stereological studies evaluating the effects of haloperidol treatment (20)(21)(22)(23).…”
SUMMARYIn recent studies, some neuroleptics have shown neurotoxic activities. Clinical and experimental studies have been carried out to investigate the effects of different neuroleptic drugs considered to affect the central nervous system. The aim of this study was to evaluate neurotoxic effects of haloperidol on hippocampal neurons. The drug was given in daily doses of either 1 or 3 mg/kg for 6 weeks to adult male guinea pigs. After treatment, all animals were anaesthetized via short inhalation of ether, and then were fixed by a mixture of 2% glutaraldehyde and +2% paraformaldehyde in 0.1 M phosphate buffer. Brains were removed from the cranium and stored in the same fixative overnight. On the following day, the CA1 region of the hippocampus was dissected out. After embedding in araldite resin and obtaining semi-thin sections, the tissues were stained with toluidine blue. The physical disector was used for measurements of nuclear height and numerical density of neurons and the sections were also evaluated histopathologically. The numerical density of neurons and nuclear height in the hippocampus for the low-dose (1 mg/kg) and high-dose (3 mg/kg) experimental groups were 12.4 mm 3 and 3.6 pm and 7.14 mm 3 and 3.56 pm, respectively. In contrast, the control group had a neuronal numerical density of 16.55 mme3 and a nuclear height of 4.09 pm. There was a significant difference in both the mean density of neurons and the mean height of nuclei between haloperidol-treated and control groups (~~0.05). There was also a statistical difference in the mean density of neurons (but not in nuclear height) when comparing the dosage of haloperidol(p
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