Does Serum Hexosaminidase Activity Play a Role in the Diagnosis of Strangulated Bowel Obstruction? An Experimental Study

Oruç, M. Tahir; Özmen, Mahir; Kazan, Orhan; Düzgün, Arife Polat; Özkara, Hatice Asuman; Arık, Deniz; Seçkin, Selda; Çoşkun, Faruk

doi:10.1023/b:ddas.0000043386.84677.0c

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…These results shown in the present study are very implicative because recent study using multiple organ failure rats demonstrates that TNF-α is elevated in both the intestinal tissue and plasma (10). In another report using intestinal epithelial cell line, TNF-α and histamine stimulation increased neutrophil adhesion to intestinal epithelial cells and this increased adhesion is inhibited by anti-CD11b and anti-CD18 monoclonal antibodies (15). It has been reported that the histamine and TNF-α released from activated the intestinal mucosal mast cells (IMMC) play an important role in the development of multiple organ failure (10).…”

Section: Discussionsupporting

confidence: 79%

“…Unfortunately, none of these markers seem not to be beneficial for the early and differen-tial diagnosis of SBO. Recent report using rat model has shown that increased serum hexosaminidase levels indicate irreversible transmural infarction only in the late period of strangulation in the closed loop SBO (15). Similar to hexosaminidase, DAO represents the biological activity in the mucosa, therefore, the present result of differential pattern of serum DAO activity might be a useful tool for diagnosis.…”

Section: Discussionmentioning

confidence: 85%

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Molecular Analysis for Differential Diagnosis of Small Bowel Obstruction: Expression of Proinflammatory Cytokines and Diamine Oxidase Activity

Takada,

Akimoto,

Ichihara

et al. 2006

Int. J Biomed Sci.

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Background: A small bowel obstruction is classified as simple (nonstrangulated) or strangulated. The early recognition with correct diagnosis of small bowel obstruction is a critical issue as the release from strangulation requires surgical emergency. Methods: To evaluate the physiological effect on small bowel obstruction, a metallic ring was put in the small intestine (simple ileus) and a loop obstruction was made with keeping the blood flow (strangulated obstruction). Serum level of cytokines, IL-6, TNF-alfa, and IL-1beta as well as endotoxin and seromuscular enzymes, CPK and LDH, were serially analyzed. Serum and mucosal DAO activity were also assessed. Results: Endotoxin was increased at 18 h through 48 h in strangulated obstruction, but not detected in the simple ileus. Early proinflammatory cytokines were significantly elevated in strangulated obstruction. High expression of IL-6 prolonged after 12 h. Transiently expressed IL-1beta peaked at 12 h, TNF-alfa? was increased at 18 h. In simple ileus, these expressions of cytokines were low and slow. LDH and CPK were significantly elevated at 48h, but there were no difference between simple ileus and strangulated obstruction. Serum DAO activity was significantly increased in simple ileus, but gradually decreased in strangulated obstruction, while mucosal DAO activity was decreased in both groups. Conclusions: High level of serum IL-6 is an early marker for strangulated obstruction. The pattern of serum DAO activity, decrease in strangulated obstruction and increase in simple ileus, might be useful molecular parameter in the early and proper diagnosis of small bowel obstruction.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 85%

Molecular Analysis for Differential Diagnosis of Small Bowel Obstruction: Expression of Proinflammatory Cytokines and Diamine Oxidase Activity

Takada,

Akimoto,

Ichihara

et al. 2006

Int. J Biomed Sci.

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“…Strangulation models [78, [91][92][93][94][95][96][97][98][99]: By definition, strangulation refers to a disruption in the blood supply to the intestine. Vascular occlusion can be achieved either by ligating or clamping the vessels.…”

Section: Complete Mechanical Obstructionmentioning

confidence: 99%

Experimental Animal Models for Studying Intestinal Obstruction

Mavrigiannaki,

Georgopoulos

2024

Animal Models in Medical Research [Working Title]

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Understanding the pathophysiology of intestinal obstruction and exploring potential therapeutic interventions heavily relies on the utilization of experimental animal models. This book chapter provides a comprehensive overview of various animal models employed in the study of surgically induced intestinal obstruction. From rodents to large animals, a range of experimental setups and methodologies are discussed, each offering unique advantages and insights into the complexities of this condition. The chapter provides a guide for researchers aiming to investigate intestinal obstruction, reviewing all aspects of an experimental protocol. Ethical and regulatory regulations, anatomical and physiological differences among species, and surgically induced experimental intestinal obstruction are reviewed. The existing experimental animal models are evaluated regarding their reproducibility and efficacy, as many published models prove difficult to replicate in the laboratory or lack crucial information for researchers. A chapter of this nature would greatly benefit the research community and pave the way for future studies.

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Does Serum Hexosaminidase Activity Play a Role in the Diagnosis of Strangulated Bowel Obstruction? An Experimental Study

Cited by 2 publications

References 16 publications

Molecular Analysis for Differential Diagnosis of Small Bowel Obstruction: Expression of Proinflammatory Cytokines and Diamine Oxidase Activity

Molecular Analysis for Differential Diagnosis of Small Bowel Obstruction: Expression of Proinflammatory Cytokines and Diamine Oxidase Activity

Experimental Animal Models for Studying Intestinal Obstruction

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