Does culture medium influence offspring birth weight?

Carrasco, Beatriz; Boada, Montserrat; Rodríguez, Ignacio; Coroleu, Buenaventura; Barri, Pedro N.; Veiga, Anna

doi:10.1016/j.fertnstert.2013.07.006

Cited by 50 publications

(34 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In clinical practice, IVF laboratories use embryo culture media whereby the nutritional content has been extrapolated from data obtained from murine embryo development (Gardner and Lane, 1998) and thus may not reflect the nutritive requirement of the developing human embryo as it moves through the reproductive tract (Houghton, 2012). Given that there is now significant, albeit controversial (Carrasco et al , 2013; Lin et al , 2013), evidence supporting the profound influence of the preimplantation environment on subsequent birthweight (Dumoulin et al , 2010; Eskild et al , 2013), it is possible that the inclusion of physiological concentrations of pleiotropic nutrients, such as amino acids, could further enhance the success of clinical IVF.…”

Section: Discussionmentioning

confidence: 99%

Amino acid composition of human uterine fluid: association with age, lifestyle and gynaecological pathology

et al. 2015

View full text Add to dashboard Cite

STUDY QUESTIONDo the amino acid levels of human uterine fluid vary with age, BMI, phase of menstrual cycle, benign pathology or diet?SUMMARY ANSWERThe levels of 18 amino acids in human uterine fluid were shown to be affected only by maternal diet.WHAT IS KNOWN ALREADYMurine, bovine and ovine uterine amino acid content has been reported, but no reliable data on the human exist. Murine studies have demonstrated that the intrauterine periconceptional nutritional environment is affected by maternal diet.STUDY DESIGN, SIZE, DURATIONUterine secretions were aspirated from 56 women aged 18–45 years. The women were recruited preoperatively from gynaecological theatre operating schedules or hysterosalpingo-contrast-sonography (HyCoSy) lists. A proportion of these women had proven fertility; however, the majority were being investigated for subfertility. The BMI, gynaecological history and dietary pattern of these women were also assessed.PARTICIPANTS/MATERIALS, SETTING, METHODSReverse phase high performance liquid chromatography was used to analyse the concentrations of 18 amino acids within the uterine fluid and blood serum. The results were analysed against the women's stage of cycle, age, BMI and diet.MAIN RESULTS AND THE ROLE OF CHANCEThe profile of 18 amino acids in uterine fluid was described. In total, human uterine fluid was observed to contain an amino acid concentration of 3.54 mM (interquartile range: 2.27–6.24 mM). The relative concentrations of 18 amino acids were not significantly altered by age, BMI, cycle phase or the presence of specific benign gynaecological pathologies. However, a diet identified by a validated scoring system as being less healthy was associated with higher concentrations of asparagine (P = 0.018), histidine (P = 0.011), serine (P = 0.033), glutamine (P = 0.049), valine (P = 0.025), phenylalanine (P = 0.019), isoleucine (P = 0.025) and leucine (P = 0.043) in the uterine fluid compared with a healthier diet, defined as one with a higher intake of fresh vegetables, fruit, whole-grain products and fish and a low intake of red and processed meat and high fat dairy products. There were no significant correlations between serum amino acid concentrations and those in the uterine fluid.LIMITATIONS, REASONS FOR CAUTIONOur results enabled us to detect the effect of diet on the concentrations of amino acids in human uterine fluid; however, the study may not have had sufficient numbers to detect mild effects of BMI or age.WIDER IMPLICATIONS OF THE FINDINGSThese findings increase our understanding of the nutritional environment encountered by the preimplantation embryo, and indicate how periconceptional diet may alter this. Given the importance of early embryo environment for programming of development and future health, this information may aid in the development of nutritional interventions aimed at optimizing the preimplantation phase of human embryo development in vivo.STUDY FUNDING/COMPETING INTEREST(S)This work was funded by the NIHR, the Medical Research Council (G0701153) and the University o...

show abstract

Section: Discussionmentioning

confidence: 99%

Amino acid composition of human uterine fluid: association with age, lifestyle and gynaecological pathology

et al. 2015

View full text Add to dashboard Cite

show abstract

“…More research needs to be performed to analyse the effects of individual culture medium formulations and to evaluate the long-term effects of embryo culture medium on the health of children conceived through ART [70,71].…”

Section: The Role Of In Vitro Embryo Nutritionmentioning

confidence: 99%

Birth defects and congenital health risks in children conceived through assisted reproduction technology (ART): a meeting report

Albertini

Evers²,

Geraedts³

et al. 2014

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Assisted Reproduction Treatment (ART) is here to stay. This review addresses the parental background of birth defects, before, during and after conception and focuses both on the underlying subfertility and on the question whether ART as a treatment is an additional contributing factor. Methods Searches were performed in Medline and other databases. Summaries were discussed in a Delphi panel set-up by the European Society of Human Reproduction and Embryology (ESHRE). Results Several birth defects and adult diseases arise during the earliest stages of ovarian development and oocyte differentiation: this is the case of cleft palate disorders in offspring from female rat exposed to Dioxin during fetal life or the polycystic ovary diseases in female offspring (primates) exposed to elevated androgen concentration during fetal life. Human oocytes and embryos often fail to stop the propagation of aneuploid cells but maintain their ability to repair DNA damages including those introduced by the fertilizing sperm. There is a 29 % increased risk of birth defects in the newborns spontaneously conceived by subfertile couples and the risk is further increased (34 %) when conception is achieved by treating infertlity with ART (Danish IVF Registry). Periconceptional conditions are critical for ART babies: their birth weight is in general smaller (Norvegian Registry) but a more prolonged culture time doubled the number of large babies (Finnish Registry). Conclusion The long-term developmental effects of ART on child and subsequent health as an adult remains a subject worthy of futher monitoring and investigation. Capsule Remaining mindful of the growing body of evidence showing that during gamete production, genetic and epigenetic changes influence offspring health, serves to reinforce the need to continue to monitor ART children.

show abstract

“…A detailed description of the components of those media is not always disclosed by the companies. Even though the main components of most commercial culture media are known, the definitive composition is complex including components such as growth factors, amino acids, serum supplements, salts, antibiotics, energy substrates, vitamins, buffer solutions, nucleotides, as well as trace elements and nuclease inhibitors, all at an undefined concentration (28,29). One of the main issues is the fact that the concentrations are unknown and may vary considerably, highlighting the diversity of the culture environment and subsequently the varied conditions that the embryos are exposed to.…”

Section: Abstract There Are Numerous Reports On Embryo Culture Mediamentioning

confidence: 99%

“…Dumoulin et al reported that use of certain commercial culture media is significantly correlated with increased birth weight compared to other media (4), which has further been confirmed (5). However, others report no association between particular culture media and neonatal birthweight (29,(33)(34)(35). An overall statement is that the nutritional composition of the culture medium has an impact on early embryonic development.…”

Section: Abstract There Are Numerous Reports On Embryo Culture Mediamentioning

confidence: 99%

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Simopoulou

Sfakianoudis²,

Rapani

et al. 2018

View full text Add to dashboard Cite

show abstract

Does culture medium influence offspring birth weight?

Cited by 50 publications

References 31 publications

Amino acid composition of human uterine fluid: association with age, lifestyle and gynaecological pathology

Amino acid composition of human uterine fluid: association with age, lifestyle and gynaecological pathology

Birth defects and congenital health risks in children conceived through assisted reproduction technology (ART): a meeting report

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Contact Info

Product

Resources

About