Docetaxel loaded chitosan nanoparticles: Formulation, characterization and cytotoxicity studies

Jain, Ankit; Thakur, Kanika; Kush, Preeti; Jain, Upendra Kumar

doi:10.1016/j.ijbiomac.2014.06.029

Cited by 73 publications

(44 citation statements)

References 33 publications

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“…Nanoparticles based on N-palmitoyl chitosan (DCX-NPa) exhibited an average hydrodynamic size of 429 ± 3.24 nm, with a polydispersity index of 0.398 and a positive Zeta potential value (+12.1 mV ± 0.38). These results are in good agreement with other reports concerning the fact that drug entrapment could induce a slightly increase of nanoparticles size (Vadlapudi et al, 2012;Jain et al, 2014) and positive Zeta potential values are given by the amount of non-substituted amino groups (Larsson et al, 2013). It has been reported that positive charged nanoparticles are able to induce cytotoxic effects and disruption of the cell membrane and seemed to be slightly more effective than anionic charged ones, even though both types of charged nanocarriers accumulated to a higher extent in tumor tissue compared with nonionic coated nanoparticles (Fröhlich, 2012).…”

Section: Particles Morphology Average Size and Surface Chargesupporting

confidence: 93%

“…Several studies have been devoted to development of active biotinylated compounds (Fernandez-Megia et al, 2007;Yang et al, 2014;Zhu et al, 2015). Docetaxel (DCX), a semisynthetic derivative of taxoid family is an important chemotherapeutic agent against breast, ovarian or lung cancer and intensively used over the past several decades (Jain et al, 2014). However, the main product of DCX with clinical use, proved to cause several adverse effects (for e.g.…”

Section: Introductionmentioning

confidence: 99%

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Biotinylated N-palmitoyl chitosan for design of drug loaded self-assembled nanocarriers

Bǎlan

Redinciuc

Tudorachi³

et al. 2016

European Polymer Journal

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Section: Particles Morphology Average Size and Surface Chargesupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Biotinylated N-palmitoyl chitosan for design of drug loaded self-assembled nanocarriers

Bǎlan

Redinciuc

Tudorachi³

et al. 2016

European Polymer Journal

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“…Solid lipids may be interacting with DTX groups. However, the FTIR spectrum of SLNs shows the characteristic peaks of similar functional groups, confirming the successful formulation of nanoparticles without any chemical interaction [38].…”

Section: Fourier Transform Infrared and Raman Spectroscopymentioning

confidence: 66%

Docetaxel-loaded solid lipid nanoparticles prevent tumor growth and lung metastasis of 4T1 murine mammary carcinoma cells

et al. 2020

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Background: Metastasis causes the most breast cancer-related deaths in women. Here, we investigated the antitumor effect of solid lipid nanoparticles (SLN-DTX) when used in the treatment of metastatic breast tumors using 4T1-bearing BALB/c mice. Results: Solid lipid nanoparticles (SLNs) were produced using the high-energy method. Compritol 888 ATO was selected as the lipid matrix, and Pluronic F127 and Span 80 as the surfactants to stabilize nanoparticle dispersion. The particles had high stability for at least 120 days. The SLNs' dispersion size was 128 nm, their polydispersity index (PDI) was 0.2, and they showed a negative zeta potential. SLNs had high docetaxel (DTX) entrapment efficiency (86%), 2% of drug loading and showed a controlled drug-release profile. The half-maximal inhibitory concentration (IC 50) of SLN-DTX against 4T1 cells was more than 100 times lower than that of free DTX after 24 h treatment. In the cellular uptake test, SLN-DTX was taken into the cells significantly more than free DTX. The accumulation in the G2-M phase was significantly higher in cells treated with SLN-DTX (73.7%) than in cells treated with free DTX (23.0%), which induced subsequent apoptosis. TEM analysis revealed that SLN-DTX internalization is mediated by endocytosis, and fluorescence microscopy showed DTX induced microtubule damage. In vivo studies showed that SLN-DTX compared to free docetaxel exhibited higher antitumor efficacy by reducing tumor volume (p < 0.0001) and also prevented spontaneous lung metastasis in 4T1 tumor-bearing mice. Histological studies of lungs confirmed that treatment with SLN-DTX was able to prevent tumor. IL-6 serum levels, ki-67 and BCL-2 expression were analyzed and showed a remarkably strong reduction when used in a combined treatment. Conclusions: These results indicate that DTX-loaded SLNs may be a promising carrier to treat breast cancer and in metastasis prevention.

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“…In those previous works, the NPs were formed in presence of MTX, showing LE values of 62% [57]. Also Jain and coworkers [59] have reported the synthesis of chitosan NPs crosslinked with glutaraldehyde using a nanoemulsion method, which were loaded with docetaxel during synthesis with LE of 65-75% and LC of 8-12%.…”

Section: Loading and Release Of Mtxmentioning

confidence: 94%

Glucose cryoprotectant affects glutathione-responsive antitumor drug release from polysaccharide nanoparticles

Curcio

Blanco-Fernandez

Costoya

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

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Docetaxel loaded chitosan nanoparticles: Formulation, characterization and cytotoxicity studies

Cited by 73 publications

References 33 publications

Biotinylated N-palmitoyl chitosan for design of drug loaded self-assembled nanocarriers

Biotinylated N-palmitoyl chitosan for design of drug loaded self-assembled nanocarriers

Docetaxel-loaded solid lipid nanoparticles prevent tumor growth and lung metastasis of 4T1 murine mammary carcinoma cells

Glucose cryoprotectant affects glutathione-responsive antitumor drug release from polysaccharide nanoparticles

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