DNAzyme-based biosensor for detection of lead ion: A review

Liang, Gang; Man, Yan; Liu, An; Jin, Xinxin; Liu, Xinhui; Pan, Ligang

doi:10.1016/j.microc.2016.12.010

Cited by 87 publications

(24 citation statements)

References 86 publications

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“…Their greater stability, more ordered structure and absence in biology have practical advantages as biomedical tools and therapeutic agents for gene silencing (2) and virus control (3). Additionally, their ease of synthesis and identification make them particularly attractive for biotechnology applications (4) and some have been developed into sensors and imaging agents (5,6) for metal ions and bacteria. Hence, a general understanding of the mechanisms whereby DNA can act as an enzyme is of stand-alone significance.…”

Section: Introductionmentioning

confidence: 99%

Dynamical ensemble of the active state and transition state mimic for the RNA-cleaving 8–17 DNAzyme in solution

Ekesan

York

2019

Nucleic Acids Research

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We perform molecular dynamics simulations, based on recent crystallographic data, on the 8–17 DNAzyme at four states along the reaction pathway to determine the dynamical ensemble for the active state and transition state mimic in solution. A striking finding is the diverse roles played by Na+ and Pb2+ ions in the electrostatically strained active site that impact all four fundamental catalytic strategies, and share commonality with some features recently inferred for naturally occurring hammerhead and pistol ribozymes. The active site Pb2+ ion helps to stabilize in-line nucleophilic attack, provides direct electrostatic transition state stabilization, and facilitates leaving group departure. A conserved guanine residue is positioned to act as the general base, and is assisted by a bridging Na+ ion that tunes the pKa and facilitates in-line fitness. The present work provides insight into how DNA molecules are able to solve the RNA-cleavage problem, and establishes functional relationships between the mechanism of these engineered DNA enzymes with their naturally evolved RNA counterparts. This adds valuable information to our growing body of knowledge on general mechanisms of phosphoryl transfer reactions catalyzed by RNA, proteins and DNA.

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Section: Introductionmentioning

confidence: 99%

Dynamical ensemble of the active state and transition state mimic for the RNA-cleaving 8–17 DNAzyme in solution

Ekesan

York

2019

Nucleic Acids Research

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“…DNAzymes that break down RNA as DNA-based catalysts are obtained through in vitro selection, which turned out to be a very useful platform for the identification of metal ions. After binding with heavy metal ions, many biochemical and biophysical studies have been conducted on DNAzymes due to their high metal ion selectivity and high catalytic efficiency [96]. Therefore, DNAzymes have been applied in various biosensors (colorimetric, electrochemical and fluorescent) that realize the detection of various metal ions such as Mg(II) [97], Ag(I) [98], Pb(II) [99], Zn(II) [100], Hg(II) [101], UO 2 (II) [102].…”

Section: Dnazymementioning

confidence: 99%

The Novel Nanomaterials Based Biosensors and Their Applications

Şensoy¹,

Muti²

2021

Novel Nanomaterials

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Since the development of the first biosensor reported, biosensor has received considerable attention due to its high selectivity and sensitivity. Biosensors are highly pursued in order to meet the growing demands and challenges in a large number of analytic applications such as medical diagnosis, food safety control, environmental monitoring, or even military defense. Due to the unique physical, chemical, mechanical and electrical properties, nanomaterials have been widely investigated for their ability and used to fabricate sensors. High surface to volume ratio, good stability, excellent electrocatalytic properties of the nanomaterials plays an important role in the sensitive and selective detection of biomolecules. The synthesis of new nanomaterials with different properties is increasingly common in order to improve these counted properties of nanomaterials. This chapter gives an overview of the importance of the development of novel nanomaterials based biosensors technologies. The use of different funtionalized carbon nanomaterilas, metal oxide nanoparticles, metal nanoparticles, polymeric nanoparticles, quantum dots, graphene sheets and other novel nanomaterials in biosensor technology, and their innovations and advantages are discussed.

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“…DNAzyme is an artificial enzyme that is made of hemin and aptamer [ 133 ]. Like peroxidase enzyme, DNAzyme that has hemin cofactor can oxidase ABTS 2− ion to ABTS − ( Figure 8 ).…”

Section: Biosensors (Biorecognizer-based Sensors)mentioning

confidence: 99%

Advances in Optical Biosensors and Sensors Using Nanoporous Anodic Alumina

Tabrizi

Ferré‐Borrull

Marsal

2020

Sensors

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This review paper focuses on recent progress in optical biosensors using self-ordered nanoporous anodic alumina. We present the fabrication of self-ordered nanoporous anodic alumina, surface functionalization, and optical sensor applications. We show that self-ordered nanoporous anodic alumina has good potential for use in the fabrication of antibody-based (immunosensor), aptamer-based (aptasensor), gene-based (genosensor), peptide-based, and enzyme-based optical biosensors. The fabricated optical biosensors presented high sensitivity and selectivity. In addition, we also showed that the performance of the biosensors and the self-ordered nanoporous anodic alumina can be used for assessing biomolecules, heavy ions, and gas molecules.

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DNAzyme-based biosensor for detection of lead ion: A review

Cited by 87 publications

References 86 publications

Dynamical ensemble of the active state and transition state mimic for the RNA-cleaving 8–17 DNAzyme in solution

Dynamical ensemble of the active state and transition state mimic for the RNA-cleaving 8–17 DNAzyme in solution

The Novel Nanomaterials Based Biosensors and Their Applications

Advances in Optical Biosensors and Sensors Using Nanoporous Anodic Alumina

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