Advances in Optical Biosensors and Sensors Using Nanoporous Anodic Alumina

Tabrizi, Mahmoud Amouzadeh; Ferré‐Borrull, Josep; Marsal, Lluís F.

doi:10.3390/s20185068

Cited by 12 publications

(7 citation statements)

References 166 publications

(176 reference statements)

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“…NAA is attractive not only due to its impressive enormous surface area combined with high chemical and thermal resistance, but due to the range of robust functionalization processes such as salinization [42], electrostatic interaction [43], and immune complexation [44] that allow versatile utility of such substrates: Sensors [45,46], templates [47], or drug delivery systems [48].…”

Section: Nanoporous Anodic Alumina (Naa): Definition and Formation Mementioning

confidence: 99%

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Recent Advances in Nanoporous Anodic Alumina: Principles, Engineering, and Applications

2021

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The development of aluminum anodization technology features many stages. With the story stretching for almost a century, rather straightforward—from current perspective—technology, raised into an iconic nanofabrication technique. The intrinsic properties of alumina porous structures constitute the vast utility in distinct fields. Nanoporous anodic alumina can be a starting point for: Templates, photonic structures, membranes, drug delivery platforms or nanoparticles, and more. Current state of the art would not be possible without decades of consecutive findings, during which, step by step, the technique was more understood. This review aims at providing an update regarding recent discoveries—improvements in the fabrication technology, a deeper understanding of the process, and a practical application of the material—providing a narrative supported with a proper background.

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Section: Nanoporous Anodic Alumina (Naa): Definition and Formation Mementioning

confidence: 99%

“…The size of NAA structures enables unique possibility to mimic and observe biological systems at the cellular level [12,45]. NAA coated with CuO and L-Cys/D-Cys with purpose to monitor response of biological molecules was demonstrated in work of Chen et a.…”

Section: Biological Monitoring and Cell Culturementioning

confidence: 99%

Recent Advances in Nanoporous Anodic Alumina: Principles, Engineering, and Applications

2021

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“…Most research that focuses on the development of optical sensors is based on porous materials that make use of top-down fabrication approaches for the creation of these porous substrates, as is the case for porous silicon (pSi) [ 14 , 15 , 16 , 17 ] and anodic aluminum oxide (AAO) [ 18 , 19 , 20 ]. These types of porous sensors exhibit several interesting properties, such as high sensitivity, good stability and chemical compatibility with different biofunctionalization routes, which make them suitable for many applications.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mesoporous TiO2 Layers as Glucose Optical Sensors

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Ponce-Alcántara

et al. 2022

Sensors

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Porous materials are currently the basis of many optical sensors because of their ability to provide a higher interaction between the light and the analyte, directly within the optical structure. In this study, mesoporous TiO2 layers were fabricated using a bottom-up synthesis approach in order to develop optical sensing structures. In comparison with more typical top-down fabrication strategies where the bulk constitutive material is etched in order to obtain the required porous medium, the use of a bottom-up fabrication approach potentially allows increasing the interconnectivity of the pore network, hence improving the surface and depth homogeneity of the fabricated layer and reducing production costs by synthesizing the layers on a larger scale. The sensing performance of the fabricated mesoporous TiO2 layers was assessed by means of the measurement of several glucose dilutions in water, estimating a limit of detection even below 0.15 mg/mL (15 mg/dL). All of these advantages make this platform a very promising candidate for the development of low-cost and high-performance optical sensors.

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“…An anodic aluminum oxide (AAO) membrane is a suitable platform to reconstruct enzyme assemblies due to the dominance of convective flow through their highly oriented monolithic channels, which facilitate efficient transport of reactive intermediates sequentially from one enzyme site to another. − Using the well-established anodization processes, the size of the monolithic channels can be modified to control enzyme load and the flow behavior. − Numerous efforts have been devoted to construct multienzyme assemblies on AAO using a variety of approaches. − One major shortcoming of these approaches, however, is the utilization of the same coupling chemistry for the entire system, which limits the immobilization of the cascading components in the correct sequence. ,,, With their exquisite material recognition and self-assembly properties, solid-binding peptides are appealing engineering tools, which can be utilized as molecular linkers to selectively immobilize biomolecules, e.g., enzymes, on a variety of solid surfaces. − In particular, by constructing AAO membranes functionalized with diverse materials, mixtures of fusion enzymes can be self-directed to immobilize on a desired target material layer, in a single step with high positional precision.…”

Section: Introductionmentioning

confidence: 99%

Solid-Binding Peptide-Guided Spatially Directed Immobilization of Kinetically Matched Enzyme Cascades in Membrane Nanoreactors

et al. 2021

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Biocatalysis is a useful strategy for sustainable green synthesis of fine chemicals due to its high catalytic rate, reaction specificity, and operation under ambient conditions. Addressable immobilization of enzymes onto solid supports for one-pot multistep biocatalysis, however, remains a major challenge. In natural pathways, enzymes are spatially coupled to prevent side reactions, eradicate inhibitory products, and channel metabolites sequentially from one enzyme to another. Construction of a modular immobilization platform enabling spatially directed assembly of multiple biocatalysts would, therefore, not only allow the development of high-efficiency bioreactors but also provide novel synthetic routes for chemical synthesis. In this study, we developed a modular cascade flow reactor using a generalizable solid-binding peptide-directed immobilization strategy that allows selective immobilization of fusion enzymes on anodic aluminum oxide (AAO) monoliths with high positional precision. Here, the lactate dehydrogenase and formate dehydrogenase enzymes were fused with substrate-specific peptides to facilitate their self-immobilization through the membrane channels in cascade geometry. Using this cascade model, two-step biocatalytic production of l-lactate is demonstrated with concomitant regeneration of soluble nicotinamide adenine dinucleotide (NADH). Both fusion enzymes retained their catalytic activity upon immobilization, suggesting their optimal display on the support surface. The 85% cascading reaction efficiency was achieved at a flow rate that kinetically matches the residence time of the slowest enzyme. In addition, 84% of initial catalytic activity was preserved after 10 days of continuous operation at room temperature. The peptide-directed modular approach described herein is a highly effective strategy to control surface orientation, spatial localization, and loading of multiple enzymes on solid supports. The implications of this work provide insight for the single-step construction of high-power cascadic devices by enabling co-expression, purification, and immobilization of a variety of engineered fusion enzymes on patterned surfaces.

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Advances in Optical Biosensors and Sensors Using Nanoporous Anodic Alumina

Cited by 12 publications

References 166 publications

Recent Advances in Nanoporous Anodic Alumina: Principles, Engineering, and Applications

Recent Advances in Nanoporous Anodic Alumina: Principles, Engineering, and Applications

Evaluation of Mesoporous TiO2 Layers as Glucose Optical Sensors

Solid-Binding Peptide-Guided Spatially Directed Immobilization of Kinetically Matched Enzyme Cascades in Membrane Nanoreactors

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